Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,570,149	C→T	intergenic (+231/+13)	rpnD → / ← yjiR	truncated recombination‑promoting nuclease‑like protein RpnD/fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,570,149	0	C	T	100.0%	319.2 / NA	91	intergenic (+231/+13)	rpnD/yjiR	truncated recombination‑promoting nuclease‑like protein RpnD/fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (55/36);  total (55/36)

ACCTGGTCAGGCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCCGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTGCCGGAAGCTGAGAAA  >  NC_000913/4570015‑4570282                                                                                                                                       |                                                                                                                                      aCCTGGTCAGGCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTAt                                                                                                                                  >  2:1014436/1‑140 (MQ=255)   cTGGTCAGGCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTAttt                                                                                                                                <  2:1464716/140‑1 (MQ=255)     ggTCAGGCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCg                                                                                                                              >  1:391089/1‑140 (MQ=255)     ggTCAGGCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCg                                                                                                                              >  2:1376447/1‑140 (MQ=255)     ggTCAGGCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCg                                                                                                                              >  2:1064355/1‑140 (MQ=255)      gTCAGGCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGa                                                                                                                             >  2:516868/1‑140 (MQ=255)      gTCAGGCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGa                                                                                                                             >  2:549113/1‑140 (MQ=255)          ggCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAAttt                                                                                                                         <  1:617461/140‑1 (MQ=255)           gCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTa                                                                                                                        >  2:712007/1‑140 (MQ=255)           gCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTa                                                                                                                        >  2:838358/1‑140 (MQ=255)             gTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTAtt                                                                                                                      >  1:411837/1‑140 (MQ=255)             gTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTAtt                                                                                                                      >  1:960825/1‑140 (MQ=255)              ttCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTc                                                                                                                     <  1:1370985/140‑1 (MQ=255)               tCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTcc                                                                                                                    >  2:624541/1‑140 (MQ=255)               tCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTcc                                                                                                                    >  1:494691/1‑140 (MQ=255)                cacaTGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCa                                                                                                                   >  2:110430/1‑140 (MQ=255)                cacaTGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCa                                                                                                                   >  2:717932/1‑140 (MQ=255)                cacaTGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCa                                                                                                                   <  1:21933/140‑1 (MQ=255)                cacaTGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCa                                                                                                                   >  2:77218/1‑140 (MQ=255)                cacaTGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCa                                                                                                                   >  2:197065/1‑140 (MQ=255)                     ggCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGcc                                                                                                              <  2:694035/140‑1 (MQ=255)                     ggCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGcc                                                                                                              >  1:935980/1‑140 (MQ=255)                     ggCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGcc                                                                                                              <  2:643906/140‑1 (MQ=255)                     ggCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGcc                                                                                                              <  1:1422751/140‑1 (MQ=255)                     ggCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGcc                                                                                                              >  1:1288508/1‑140 (MQ=255)                     ggCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGcc                                                                                                              <  2:152368/140‑1 (MQ=255)                      gCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGccc                                                                                                             <  2:1021050/140‑1 (MQ=255)                                tGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGc                                                                                                   <  2:526760/140‑1 (MQ=255)                                tGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGc                                                                                                   <  2:142003/140‑1 (MQ=255)                                  aaCAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCt                                                                                                 >  1:823815/1‑140 (MQ=255)                                   aCAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTc                                                                                                <  2:112122/140‑1 (MQ=255)                                    cAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCg                                                                                               >  1:153732/1‑140 (MQ=255)                                          gTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAAtt                                                                                         <  1:40254/140‑1 (MQ=255)                                          gTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAAtt                                                                                         <  1:1376447/140‑1 (MQ=255)                                            aCTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTg                                                                                       >  1:1163343/1‑140 (MQ=255)                                            aCTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTg                                                                                       >  1:72754/1‑140 (MQ=255)                                                 gCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCa                                                                                  >  1:64905/1‑140 (MQ=255)                                                    tAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTg                                                                               >  2:575527/1‑140 (MQ=255)                                                      aTTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCt                                                                             <  2:1127273/140‑1 (MQ=255)                                                      aTTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCt                                                                             <  1:197065/140‑1 (MQ=255)                                                      aTTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCt                                                                             <  1:1187780/140‑1 (MQ=255)                                                           ggCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTcgcg                                                                        <  1:114526/140‑1 (MQ=255)                                                           ggCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTcgcg                                                                        <  2:278286/140‑1 (MQ=255)                                                            gCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGa                                                                       >  1:530215/1‑140 (MQ=255)                                                            gCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGa                                                                       >  1:1264216/1‑140 (MQ=255)                                                             cTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGAt                                                                      <  1:1515282/140‑1 (MQ=255)                                                                 aaCGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGAt                                                                      >  2:363334/1‑136 (MQ=255)                                                                 aaCGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGAt                                                                      <  1:363334/136‑1 (MQ=255)                                                                 aaCGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGt                                                                  >  2:1438480/1‑140 (MQ=255)                                                                      ggCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGc                                                             >  2:503989/1‑140 (MQ=255)                                                                      ggCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGc                                                             >  1:1432715/1‑140 (MQ=255)                                                                          aTGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAg                                                         <  2:908666/140‑1 (MQ=255)                                                                           tGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGc                                                        >  1:1069612/1‑140 (MQ=255)                                                                               ccAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCa                                                    <  1:756722/140‑1 (MQ=255)                                                                                 aGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCaaa                                                  >  1:321050/1‑140 (MQ=255)                                                                                 aGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCaaa                                                  >  2:514967/1‑140 (MQ=255)                                                                                   cAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAgc                                                <  1:155379/140‑1 (MQ=255)                                                                                     aaGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAgcgc                                              <  2:1010250/140‑1 (MQ=255)                                                                                        aTAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGt                                           >  1:583228/1‑140 (MQ=255)                                                                                            aaCGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGAt                                       <  1:250787/140‑1 (MQ=255)                                                                                            aaCGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGAt                                       >  1:1227232/1‑140 (MQ=255)                                                                                             aCGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATg                                      >  1:527136/1‑140 (MQ=255)                                                                                             aCGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATg                                      >  1:711632/1‑140 (MQ=255)                                                                                               gAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATgcg                                    <  2:823815/140‑1 (MQ=255)                                                                                                aGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATgcgc                                   >  2:1488725/1‑140 (MQ=255)                                                                                                 gTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCa                                  <  1:1064355/140‑1 (MQ=255)                                                                                                   cacaGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGa                                <  1:899616/140‑1 (MQ=255)                                                                                                   cacaGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGa                                <  2:411837/140‑1 (MQ=255)                                                                                                   cacaGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGa                                >  2:437160/1‑140 (MQ=255)                                                                                                      aGGTTATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACaa                             <  2:1288508/140‑1 (MQ=255)                                                                                                         ttATGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTa                          <  2:1396465/140‑1 (MQ=255)                                                                                                           aTGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACg                        >  2:527663/1‑140 (MQ=255)                                                                                                            tGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACgg                       >  1:1008214/1‑140 (MQ=255)                                                                                                            tGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACgg                       >  2:9483/1‑140 (MQ=255)                                                                                                            tGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACgg                       >  2:1179619/1‑140 (MQ=255)                                                                                                            tGCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACgg                       >  2:1291639/1‑140 (MQ=255)                                                                                                             gCATGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGt                      <  1:717932/140‑1 (MQ=255)                                                                                                                tGAGAGGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTAtt                   >  2:28867/1‑140 (MQ=255)                                                                                                                  agagGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGa                                >  2:275882/1‑125 (MQ=255)                                                                                                                  agagGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGa                                <  1:275882/125‑1 (MQ=255)                                                                                                                  agagGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTg                 >  1:977950/1‑140 (MQ=255)                                                                                                                  agagGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTg                 >  1:799749/1‑140 (MQ=255)                                                                                                                  agagGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTg                 >  1:771727/1‑140 (MQ=255)                                                                                                                  agagGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTg                 >  1:103044/1‑140 (MQ=255)                                                                                                                   gagGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTGc                >  1:1485320/1‑140 (MQ=255)                                                                                                                    agGAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTGcc               >  1:405315/1‑140 (MQ=255)                                                                                                                      gAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTGCCgg             <  2:494691/140‑1 (MQ=255)                                                                                                                      gAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTGCCgg             >  1:195929/1‑140 (MQ=255)                                                                                                                      gAAATCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTGCCgg             >  1:195404/1‑140 (MQ=255)                                                                                                                        aaTCAGGCGCTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTGCCGGaa           >  1:1147558/1‑140 (MQ=255)                                                                                                                                 cTTCGCTGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTGCCGGAAGCTGAGaaa  <  1:575527/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      ACCTGGTCAGGCGTTCACATGGCATCCGGCATGAACAAAGCGTACTTTGCTTAATTCAGGCTGGAACGTGGCGATGACCCAGCAAAGATAAAACGAGTCACAGGTTATGCATGAGAGGAAATCAGGCGCTTCGCCGCTATTTCGAATTTATTCCATTGCCCGATACACGGCCTCGCCAATTTGCTTCAGTGCTTCGCGATAGGTTTCGCTGAGCGGCAAAGCGCAGTTGATGCGCAGACAATTACGGTATTTGCCGGAAGCTGAGAAA  >  NC_000913/4570015‑4570282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑