Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,721	T→C	L23L (TTA→TTG)	ytjB ←	protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,721	0	T	C	100.0%	284.1 / NA	75	L23L (TTA→TTG)	ytjB	protein Smp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/36);  total (39/36)

GTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCG  >  NC_000913/4624585‑4624856                                                                                                                                         |                                                                                                                                        gTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGCGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAAc                                                                                                                                      >  1:1252668/1‑140 (MQ=255)   gAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACcaacaa                                                                                                                                    >  1:440799/1‑140 (MQ=255)     gTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAgg                                                                                                                                  <  1:373049/140‑1 (MQ=255)     gTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAgg                                                                                                                                  <  2:837092/140‑1 (MQ=255)     gTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAgg                                                                                                                                  <  2:33350/140‑1 (MQ=255)          tACGCATCAGCGGTGCGACGTTCAGCGTCACCTGGCGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGa                                                                                                                             <  2:659716/140‑1 (MQ=255)          tACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGa                                                                                                                             <  2:598915/140‑1 (MQ=255)             gCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAg                                                                                                                          <  2:216087/140‑1 (MQ=255)             gCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAg                                                                                                                          <  1:907614/140‑1 (MQ=255)              cATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGa                                                                                                                         >  1:662934/1‑140 (MQ=255)              cATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGa                                                                                                                         >  1:849019/1‑140 (MQ=255)               aTCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGaa                                                                                                                        <  2:96423/140‑1 (MQ=255)               aTCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGaa                                                                                                                        <  2:1112627/140‑1 (MQ=255)               aTCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGaa                                                                                                                        <  2:298550/140‑1 (MQ=255)                tCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCGGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAAc                                                                                                                       <  2:1158809/140‑1 (MQ=255)                tCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAAc                                                                                                                       <  2:1246060/140‑1 (MQ=255)                    cGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGa                                                                                                                   >  2:1441532/1‑140 (MQ=255)                    cGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGa                                                                                                                   <  1:1181694/140‑1 (MQ=255)                    cGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGa                                                                                                                   >  1:1405472/1‑140 (MQ=255)                       tGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACaa                                                                                                                >  1:504994/1‑140 (MQ=255)                       tGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACaa                                                                                                                >  2:1008765/1‑140 (MQ=255)                       tGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACACTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACaa                                                                                                                >  2:1463805/1‑140 (MQ=255)                                 aGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCg                                                                                                      >  1:1343916/1‑140 (MQ=255)                                 aGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCg                                                                                                      >  2:1501100/1‑140 (MQ=255)                                        ccTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGc                                                                                               >  2:1152148/1‑140 (MQ=255)                                           gACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCgg                                                                                            <  2:32095/140‑1 (MQ=255)                                           gACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCgg                                                                                            <  1:1390704/140‑1 (MQ=255)                                                gcCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAAttt                                                                                       >  2:1469590/1‑140 (MQ=255)                                                 ccAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTc                                                                                      >  2:1423792/1‑140 (MQ=255)                                                 ccAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTc                                                                                      >  1:700332/1‑140 (MQ=255)                                                         cGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTtgt                                                                              >  1:279831/1‑140 (MQ=255)                                                         cGGGCCAGTTCTTCCAGCTGTGGATTACACTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTtgt                                                                              <  1:1463805/140‑1 (MQ=255)                                                          gggCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTtgtg                                                                             >  1:733350/1‑140 (MQ=255)                                                          gggCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTtgtg                                                                             >  2:363634/1‑140 (MQ=255)                                                           ggCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGTCCGATGCAGCCGGAATTTCAGTTTTGTGc                                                                            <  2:21105/140‑1 (MQ=255)                                                           ggCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGc                                                                            <  1:1440381/140‑1 (MQ=255)                                                              cAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAg                                                                         <  1:339981/140‑1 (MQ=255)                                                              cAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAg                                                                         <  2:822260/140‑1 (MQ=255)                                                               aGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGc                                                                        <  2:700332/140‑1 (MQ=255)                                                                 ttcttcCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCa                                                                      >  2:1042868/1‑140 (MQ=255)                                                                 ttcttcCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCa                                                                      >  1:611014/1‑140 (MQ=255)                                                                 ttcttcCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCa                                                                      >  1:901588/1‑140 (MQ=255)                                                                 ttcttcCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCa                                                                      >  1:769172/1‑140 (MQ=255)                                                                 ttcttcCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCa                                                                      >  1:806658/1‑140 (MQ=255)                                                                           tgtgGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCAccc                                                            >  2:552792/1‑140 (MQ=255)                                                                            gtgGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCt                                                           <  2:662934/140‑1 (MQ=255)                                                                             tgGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCtt                                                          >  2:704397/1‑140 (MQ=255)                                                                              ggATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCttt                                                         >  2:1306740/1‑140 (MQ=255)                                                                                    cGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAAt                                                   >  1:271024/1‑140 (MQ=255)                                                                                       tGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTg                                                <  2:1102059/140‑1 (MQ=255)                                                                                       tGTCGCTGGAGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTg                                                >  2:838119/1‑140 (MQ=255)                                                                                         tCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTgag                                              >  1:173996/1‑140 (MQ=255)                                                                                         tCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTgag                                              >  2:453880/1‑140 (MQ=255)                                                                                                 ggtTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATg                                      <  1:523567/140‑1 (MQ=255)                                                                                                 ggtTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATg                                      >  2:573425/1‑140 (MQ=255)                                                                                                              ccATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCaa                         <  2:769172/140‑1 (MQ=255)                                                                                                               cATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAAt                        <  2:849019/140‑1 (MQ=255)                                                                                                               cATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAAt                        <  2:173996/140‑1 (MQ=255)                                                                                                               cATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAAt                        <  2:1504108/140‑1 (MQ=255)                                                                                                               cATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAAt                        <  2:1033877/140‑1 (MQ=255)                                                                                                               cATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAAt                        <  2:1252668/140‑1 (MQ=255)                                                                                                               cATGACGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAAt                        <  1:1441532/140‑1 (MQ=255)                                                                                                                  gACGCTCCCTTCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACGGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGGGCCATCTTCCTCCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGa                     <  2:824947/140‑1 (MQ=255)                                                                                                                    cGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATa                   <  2:1405472/140‑1 (MQ=255)                                                                                                                    cGCTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATa                   <  2:733350/140‑1 (MQ=255)                                                                                                                      cTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACa                 <  2:901588/140‑1 (MQ=255)                                                                                                                      cTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACa                 <  1:1125589/140‑1 (MQ=255)                                                                                                                      cTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACa                 >  1:1200223/1‑140 (MQ=255)                                                                                                                      cTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACa                 >  2:1201107/1‑140 (MQ=255)                                                                                                                      cTCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACa                 >  1:875973/1‑140 (MQ=255)                                                                                                                       tCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACaa                >  1:35256/1‑140 (MQ=255)                                                                                                                       tCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACaa                >  2:596544/1‑140 (MQ=255)                                                                                                                       tCCCTGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACaa                >  1:32871/1‑140 (MQ=255)                                                                                                                           tGCATCAGCGCCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTa            >  2:982150/1‑140 (MQ=255)                                                                                                                                   cgcCACCAACAAGGCAAGACAGGACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATg    >  1:1292553/1‑140 (MQ=255)                                                                                                                                   cgcCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATg    >  2:854250/1‑140 (MQ=255)                                                                                                                                   cgcCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATg    >  2:1348961/1‑140 (MQ=255)                                                                                                                                    gcCACCAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGc   <  2:1467999/140‑1 (MQ=255)                                                                                                                                     ccaccaACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCg  >  2:666009/1‑140 (MQ=255)                                                                                                                                     ccaccaACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCg  >  1:1230530/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCG  >  NC_000913/4624585‑4624856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑