Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	615,816	T→C	S554P (TCG→CCG)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	615,816	0	T	C	100.0%	191.9 / NA	51	S554P (TCG→CCG)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/30);  total (21/30)

GGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACGGC  >  NC_000913/615680‑615949                                                                                                                                         |                                                                                                                                      ggCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCcgccgc                                                                                                                                    <  1:891665/140‑1 (MQ=255) ggCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCcgccgc                                                                                                                                    <  2:642936/140‑1 (MQ=255) ggCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCcgccgc                                                                                                                                    >  1:1456352/1‑140 (MQ=255)   cGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTg                                                                                                                                  >  2:3515/1‑140 (MQ=255)         cACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAAtt                                                                                                                            <  2:465100/140‑1 (MQ=255)           cTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTac                                                                                                                          <  1:110086/140‑1 (MQ=255)              ccGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTaccac                                                                                                                       <  1:261793/140‑1 (MQ=255)               cgcgCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTaccacc                                                                                                                      <  1:449849/140‑1 (MQ=255)                 cgcTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACcga                                                                                                                    >  1:1228039/1‑140 (MQ=255)                      ggTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATc                                                                                                               <  1:1066075/140‑1 (MQ=255)                             ttGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGcc                                                                                                        <  1:977344/140‑1 (MQ=255)                              tGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCcg                                                                                                       <  1:973866/140‑1 (MQ=255)                              tGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCcg                                                                                                       <  1:126357/140‑1 (MQ=255)                                aCCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCcgcg                                                                                                     <  1:590884/140‑1 (MQ=255)                                  ccTGGCACTCCATGCGATAGTTGACGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAAGGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCt                                                                                                   >  2:330814/1‑140 (MQ=255)                                  ccTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGTCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCt                                                                                                   >  2:810005/1‑140 (MQ=255)                                       cACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGc                                                                                              >  2:556129/1‑140 (MQ=255)                                        aCTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCg                                                                                             <  1:407254/140‑1 (MQ=255)                                                 gATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCa                                                                                    <  1:757273/140‑1 (MQ=255)                                                    aGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAAtt                                                                                 >  1:214292/1‑140 (MQ=255)                                                      ttGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTa                                                                               >  1:746949/1‑140 (MQ=255)                                                       tGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTaa                                                                              <  2:14999/140‑1 (MQ=255)                                                            cTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGc                                                                         <  1:1302284/140‑1 (MQ=255)                                                            cTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGc                                                                         <  2:1358934/140‑1 (MQ=255)                                                             tGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGcc                                                                        >  1:896214/1‑140 (MQ=255)                                                             tGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGcc                                                                        >  2:1321220/1‑140 (MQ=255)                                                               gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      >  2:1083374/1‑140 (MQ=255)                                                               gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      >  2:84564/1‑140 (MQ=255)                                                               gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      <  2:782814/140‑1 (MQ=255)                                                               gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      <  2:973889/140‑1 (MQ=255)                                                               gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      >  2:698227/1‑140 (MQ=255)                                                               gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      <  1:724717/140‑1 (MQ=255)                                                                     ccTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCATCCGCCACTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCtat                                                                >  1:1178432/1‑140 (MQ=255)                                                                           tACCGCTGGATACCGGCTCTCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCACCTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGcc                                                          <  1:810005/140‑1 (MQ=255)                                                                             ccGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCATTGCTATAACGcccc                                                        <  2:600887/140‑1 (MQ=255)                                                                               gCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGc                                                      >  2:70678/1‑140 (MQ=255)                                                                                  ggATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTa                                                   <  1:330814/140‑1 (MQ=255)                                                                                    aTACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTaca                                                 >  1:173350/1‑140 (MQ=255)                                                                                        cGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGc                                             >  1:1177644/1‑140 (MQ=255)                                                                                                    cGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTgcgc                                 <  2:746949/140‑1 (MQ=255)                                                                                                        cGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCt                             >  1:914731/1‑140 (MQ=255)                                                                                                         gCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTg                            <  1:758203/140‑1 (MQ=255)                                                                                                              aaaaTGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACt                       <  1:84564/140‑1 (MQ=255)                                                                                                                  tgatgCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTca                   >  1:1167956/1‑140 (MQ=255)                                                                                                                          gAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCa           <  2:214292/140‑1 (MQ=255)                                                                                                                          gAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCa           >  1:1247739/1‑140 (MQ=255)                                                                                                                               tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      <  1:338450/140‑1 (MQ=255)                                                                                                                               tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      <  1:3515/140‑1 (MQ=255)                                                                                                                               tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      >  1:283755/1‑140 (MQ=255)                                                                                                                               tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      <  2:182706/140‑1 (MQ=255)                                                                                                                               tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      <  1:698227/140‑1 (MQ=255)                                                                                                                               tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      >  2:1353512/1‑140 (MQ=255)                                                                                                                               tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      >  1:784809/1‑140 (MQ=255)                                                                                                                               tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      <  1:1109975/140‑1 (MQ=255)                                                                                                                                  gcgTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACgg   <  2:1456352/140‑1 (MQ=255)                                                                                                                                  atggccgccgCGGTTAAATACCACCGACTGAGAACTGCCGCGCTTAAGCGCTGTGCCCAATTTAACAAGCCTTTGCTCTAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACgg   <  2:722983/136‑1 (MQ=255)                                                                                                                                   cgtccgccGCTGTTAATTACCACCGACGATCCACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACGGc  <  2:914731/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACGGC  >  NC_000913/615680‑615949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑