Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,059,166	A→G	K361K (AAA→AAG)	torC →	cytochrome c menaquinol dehydrogenase TorC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,059,166	0	A	G	100.0%	234.8 / NA	62	K361K (AAA→AAG)	torC	cytochrome c menaquinol dehydrogenase TorC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/36);  total (26/36)

ATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAACGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGG  >  NC_000913/1059032‑1059301                                                                                                                                       |                                                                                                                                        aTCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTga                                                                                                                                    >  1:1027517/1‑140 (MQ=255) aTCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTga                                                                                                                                    >  1:1383803/1‑140 (MQ=255) aTCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTga                                                                                                                                    >  1:982978/1‑140 (MQ=255)         ttATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAGCGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCa                                                                                                                            <  2:260827/140‑1 (MQ=255)         ttATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCa                                                                                                                            <  1:882365/140‑1 (MQ=255)         ttATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCa                                                                                                                            <  1:899166/140‑1 (MQ=255)         ttATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCa                                                                                                                            <  2:69543/140‑1 (MQ=255)           aTGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCAcc                                                                                                                          <  1:627838/140‑1 (MQ=255)           aTGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCAcc                                                                                                                          <  2:1126750/140‑1 (MQ=255)            tGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCt                                                                                                                         >  2:1253582/1‑140 (MQ=255)            tGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCt                                                                                                                         >  2:747891/1‑140 (MQ=255)                gATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCttgtt                                                                                                                     >  2:1526383/1‑140 (MQ=255)                 aTTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCttgttg                                                                                                                    >  2:85341/1‑140 (MQ=255)                 aTTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCttgttg                                                                                                                    <  1:835664/140‑1 (MQ=255)                   tCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGaa                                                                                                                  >  1:87211/1‑140 (MQ=255)                      ttGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAata                                                                                                               >  2:466325/1‑140 (MQ=255)                                 cACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAaatga                                                                                                    >  1:1413617/1‑140 (MQ=255)                                 cACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAaatga                                                                                                    <  1:383801/140‑1 (MQ=255)                                 cACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAaatga                                                                                                    <  1:1277999/140‑1 (MQ=255)                                 cACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAaatga                                                                                                    >  2:487524/1‑140 (MQ=255)                                   ccTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAaatgaat                                                                                                  <  1:1334436/140‑1 (MQ=255)                                   ccTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAaatgaat                                                                                                  >  2:172615/1‑140 (MQ=255)                                          ccAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTg                                                                                           <  1:830764/140‑1 (MQ=255)                                          ccAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTg                                                                                           >  1:1149329/1‑140 (MQ=255)                                           cAGTGCGACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGa                                                                                          <  2:1074326/140‑1 (MQ=255)                                              tGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGacac                                                                                       >  1:758852/1‑140 (MQ=255)                                               gCCACGGCGCACCGGAAATCGCCCACTTTGACGGTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCGCCTTGTTGAAATATCTGCAAATGAATGCGTCTGACAcc                                                                                      <  2:382300/140‑1 (MQ=255)                                                 cACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGc                                                                                    <  1:217630/140‑1 (MQ=255)                                                   cGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAg                                                                                  >  2:317014/1‑140 (MQ=255)                                                     gcgcACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGt                                                                                <  1:869962/140‑1 (MQ=255)                                                     gcgcACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGt                                                                                <  1:1324743/140‑1 (MQ=255)                                                     gcgcACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGt                                                                                <  2:138389/140‑1 (MQ=255)                                                             gAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCa                                                                        <  2:963992/140‑1 (MQ=255)                                                                aTCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACgg                                                                     <  1:289070/140‑1 (MQ=255)                                                                aTCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACgg                                                                     <  2:1272895/140‑1 (MQ=255)                                                                aTCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACgg                                                                     <  1:591855/140‑1 (MQ=255)                                                                aTCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACgg                                                                     <  2:1440993/140‑1 (MQ=255)                                                                    cccACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGAt                                                                 <  1:615869/140‑1 (MQ=255)                                                                           tGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAgaagg                                                          <  1:1345733/140‑1 (MQ=255)                                                                                cTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGgaagaa                                                     <  1:163852/140‑1 (MQ=255)                                                                                cTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGgaagaa                                                     <  1:1526383/140‑1 (MQ=255)                                                                                cTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGgaagaa                                                     <  1:1253582/140‑1 (MQ=255)                                                                                        tGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATg                                             <  2:1383803/140‑1 (MQ=255)                                                                                          gATCGGCACGCTCAACGGCATGATTGACTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGaa                                           <  2:312738/140‑1 (MQ=255)                                                                                           aTCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAAc                                          >  2:67283/1‑140 (MQ=255)                                                                                            tCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACa                                         <  1:747891/140‑1 (MQ=255)                                                                                                  cGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACg                                   <  1:979265/140‑1 (MQ=255)                                                                                                    cTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGAt                                 <  1:262374/140‑1 (MQ=255)                                                                                                    cTCAACGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGAt                                 <  1:148338/140‑1 (MQ=255)                                                                                                       aaCGGCATGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGAtctc                              >  1:219915/1‑140 (MQ=255)                                                                                                             aTGATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAg                        >  1:1227583/1‑140 (MQ=255)                                                                                                               gATTGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGc                      >  2:494268/1‑140 (MQ=255)                                                                                                                 ttGGCTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCAt                    <  2:1486296/140‑1 (MQ=255)                                                                                                                     cTTTACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACg                <  2:982978/140‑1 (MQ=255)                                                                                                                      tttACCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGt               >  1:1466476/1‑140 (MQ=255)                                                                                                                         aCCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCgg            >  2:680644/1‑140 (MQ=255)                                                                                                                         aCCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCgg            >  2:847131/1‑140 (MQ=255)                                                                                                                         aCCAGTCTCGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCgg            >  2:920220/1‑140 (MQ=255)                                                                                                                              tctcGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGttt       >  2:1154317/1‑140 (MQ=255)                                                                                                                              tctcGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGttt       >  2:956408/1‑140 (MQ=255)                                                                                                                               ctcGATAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGtttt      <  1:788660/140‑1 (MQ=255)                                                                                                                                   aTAAGCGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTgg  >  2:589916/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        ATCTGGGCTTATGCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCACTTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGATAAACGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGG  >  NC_000913/1059032‑1059301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑