Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,159,785	A→G	S589S (AGT→AGC)	fhuE ←	ferric coprogen/ferric rhodotorulic acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,785	0	A	G	100.0%	200.5 / NA	53	S589S (AGT→AGC)	fhuE	ferric coprogen/ferric rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/29);  total (24/29)

CATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTACTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAGCGACATTATCCTGCTCAATACGGAAGATGGCTAACGTGGTGGTCAGACGGCTATTCAT  >  NC_000913/1159650‑1159916                                                                                                                                        |                                                                                                                                    cATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTgctga                                                                                                                                 <  1:1359105/140‑1 (MQ=255) cATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTgctga                                                                                                                                 <  1:1382046/140‑1 (MQ=255)   ttttAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTgctgact                                                                                                                               <  2:711420/140‑1 (MQ=255)           gtggtgCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATc                                                                                                                       >  1:21711/1‑140 (MQ=255)            tggtgCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATcc                                                                                                                      >  2:1459502/1‑140 (MQ=255)            tggtgCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATcc                                                                                                                      >  2:123774/1‑140 (MQ=255)              gtgCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCAc                                                                                                                    <  1:153619/140‑1 (MQ=255)              gtgCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCAc                                                                                                                    <  2:1167406/140‑1 (MQ=255)              gtgCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCAc                                                                                                                    >  1:1388053/1‑140 (MQ=255)                  gTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCt                                                                                                                <  1:549277/140‑1 (MQ=255)                   tGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCtt                                                                                                               >  1:465120/1‑140 (MQ=255)                         aTTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAgg                                                                                                         >  1:610156/1‑140 (MQ=255)                           tAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCg                                                                                                       >  1:1200845/1‑140 (MQ=255)                             ggATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGt                                                                                                     <  2:707093/140‑1 (MQ=255)                                   aCGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCCGCCAGTTGGCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTcccc                                                                                               >  2:882558/1‑140 (MQ=255)                                       cGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTtg                                                                                           <  2:633599/140‑1 (MQ=255)                                         tttCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTTGCCGTtgtt                                                                                         >  1:449018/1‑138 (MQ=255)                                         tttCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCgctgct                                                                                         >  1:1519992/1‑140 (MQ=255)                                          ttCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTtgctg                                                                                        <  2:544971/140‑1 (MQ=255)                                               tCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCggg                                                                                   <  1:1312169/140‑1 (MQ=255)                                                  ttATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGAt                                                                                <  2:657239/140‑1 (MQ=255)                                                  ttATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGAt                                                                                <  1:66331/140‑1 (MQ=255)                                                  ttATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGAt                                                                                <  2:874791/140‑1 (MQ=255)                                                  ttATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGAt                                                                                <  2:631205/140‑1 (MQ=255)                                                  ttATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGAt                                                                                <  1:385689/140‑1 (MQ=255)                                                  ttATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGAt                                                                                <  1:1276931/140‑1 (MQ=255)                                                  ttATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGAt                                                                                <  2:963619/140‑1 (MQ=255)                                                  ttATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGAt                                                                                <  1:1457483/140‑1 (MQ=255)                                                     tCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGGTAgg                                                                             >  1:779291/1‑140 (MQ=255)                                                       ctctGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGgtg                                                                           <  1:286164/140‑1 (MQ=255)                                                       ctctGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGgtg                                                                           <  1:212835/140‑1 (MQ=255)                                                               tataGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGGTAGGGGTACCGGTg                                                                   >  1:97950/1‑140 (MQ=255)                                                                    cgcgTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCTCCGTTGCTGCCGGGGGTAGGTGTACCGGGGGACTg                                                              >  2:645760/1‑140 (MQ=255)                                                                      cgTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCTCCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAg                                                            <  1:730371/140‑1 (MQ=255)                                                                      cgTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGGTAGGGGTACCGGTGGACTGAg                                                            >  1:1251722/1‑140 (MQ=255)                                                                      cgTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAg                                                            <  1:1459502/140‑1 (MQ=255)                                                                      cgTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAg                                                            <  1:123774/140‑1 (MQ=255)                                                                      cgTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAg                                                            <  2:1495350/140‑1 (MQ=255)                                                                      cgTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGGACCGGTGGACTGAg                                                            >  1:1082811/1‑140 (MQ=255)                                                                      cgTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCCTTGCTGCCGGGGGTTGGGGTACCGGGGGGATGAg                                                            >  2:1153009/1‑140 (MQ=255)                                                                      cgTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCCCTGCTGCCGGGGGTAGGGGGACCGgggggccggg                                                            >  2:264495/1‑134 (MQ=255)                                                                         tGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTTGCCGTTGCTGCCGGGGATATGTGGACCGGGGGGCTGAGGGa                                                         >  2:359467/1‑140 (MQ=255)                                                                         tGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGGTAGGGGTACCGGTGGGCTGAGCgg                                                         >  1:1076418/1‑139 (MQ=255)                                                                           cgcCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCTCCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAGCGACa                                                       <  2:1253520/140‑1 (MQ=255)                                                                           cgcCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGGGTACCGGGGGACTGAGCGACa                                                       >  1:243313/1‑140 (MQ=255)                                                                                aaTGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAGCGACATTATc                                                  <  2:729983/140‑1 (MQ=255)                                                                                aaTGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAGCGACATTATc                                                  <  2:976293/140‑1 (MQ=255)                                                                                aaTGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAGCGACATTATc                                                  <  1:703134/140‑1 (MQ=255)                                                                                aaTGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAGCGACATTATc                                                  <  1:6260/140‑1 (MQ=255)                                                                                     cAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGGTGCTGCCGGGGGTAGGGGGACCGGGGGGCTGAGCGGCATTATCCTGcc                                             >  2:575268/1‑139 (MQ=255)                                                                                      aGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGGTAGGTGTACCGGTGGGCTGAGCGACATTATCCTGCTc                                            >  1:1121760/1‑140 (MQ=255)                                                                                      aGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGGTAGGGGTACCGGTGGACTGAGCGACATTATCCTGCTc                                            >  1:1385944/1‑140 (MQ=255)                                                                                             aGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTTGCCGCTGCTGCCTGGGGCAGGTGTAACGGTGGGCTGGGCGGCACTATTCTTCTCCATACgg                                     >  2:1249012/1‑140 (MQ=255)                                                                                                tGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGAACTGAGCGACATTATCCTGCTCAATACGGAAg                                  <  1:991184/140‑1 (MQ=255)                                                                                                                  tGAGTTCAAATTCCACCCCTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAGCGACATTATCCTGCTCAATACGGAAGATGGCTAACGTGGTGGTc                <  1:452770/140‑1 (MQ=255)                                                                                                                       tCAAATTCCACCCCTTTGCGGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTGCCGGTGGACTGAGCGACATTATCCTGCTCAATACGGAAGATGGCTAACGTGGTGGTCAGACg           <  2:1224522/140‑1 (MQ=255)                                                                                                                                cccccTTTGCTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAGCGACATTATCCTGCTCAATACGGAAGATGGCTAACGTGGTGGTCAGACGGCTATTCAt  <  2:21711/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCACCCCTTTACTGACTGTCCCATCCACCGCTTTATAGGCGGTTTCGCCGTTGCTGCCGGGGATAGGTGTACCGGTGGACTGAGCGACATTATCCTGCTCAATACGGAAGATGGCTAACGTGGTGGTCAGACGGCTATTCAT  >  NC_000913/1159650‑1159916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑