Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,351	C→T	E414K (GAA→AAA)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,351	0	C	T	100.0%	192.4 / NA	56	E414K (GAA→AAA)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/29);  total (27/29)

TAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCT  >  NC_000913/1541219‑1541486                                                                                                                                     |                                                                                                                                        tAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTACGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTcc                                                                                                                                  >  1:913608/1‑140 (MQ=255)       gCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACcg                                                                                                                            >  2:403286/1‑140 (MQ=255)              gcgcACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCaa                                                                                                                     <  1:379415/140‑1 (MQ=255)                gcACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCACCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATg                                                                                                                   <  1:318508/140‑1 (MQ=255)                 cACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATgg                                                                                                                  <  1:1299281/140‑1 (MQ=255)                 cACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATgg                                                                                                                  >  2:118558/1‑140 (MQ=255)                           ggATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGCCCCCGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGgattccagatt                                                                                                        >  2:417691/1‑140 (MQ=255)                            gATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGgattccagattc                                                                                                       >  1:1137838/1‑140 (MQ=255)                                gtgtTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCATCAGCGTCATGTTGACCGAGCAGGGTTACCGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCAtt                                                                                                   <  2:164006/140‑1 (MQ=255)                                gtgtTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCAtt                                                                                                   >  1:1276987/1‑140 (MQ=255)                                 tgtTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCAttt                                                                                                  >  2:1441021/1‑140 (MQ=255)                                   ttttACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGc                                                                                                <  1:664639/140‑1 (MQ=255)                                        cGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCtttt                                                                                           >  1:813655/1‑140 (MQ=255)                                           tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTtct                                                                                        >  2:191552/1‑140 (MQ=255)                                            gCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTtctc                                                                                       <  2:415853/140‑1 (MQ=255)                                             cGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTcc                                                                                      >  1:1095371/1‑140 (MQ=255)                                              gAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTccc                                                                                     <  2:1066441/140‑1 (MQ=255)                                                           ttCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCaa                                                                        <  1:156140/140‑1 (MQ=255)                                                               aTGCCGCCAAAGTAGGGGAAGGCCACGCCTGCGACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGa                                                                    >  1:276210/1‑140 (MQ=255)                                                                  ccgccAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAAcc                                                                 >  1:1039565/1‑140 (MQ=255)                                                                  ccgccAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAAcc                                                                 >  1:1167111/1‑140 (MQ=255)                                                                    gccAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGt                                                               <  1:1054799/140‑1 (MQ=255)                                                                    gccAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGt                                                               <  2:1137838/140‑1 (MQ=255)                                                                       aaaGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCg                                                            <  1:798568/140‑1 (MQ=255)                                                                        aaGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCgg                                                           <  1:1020151/140‑1 (MQ=255)                                                                        aaGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCgg                                                           <  2:675085/140‑1 (MQ=255)                                                                         aGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGc                                                          <  1:16271/140‑1 (MQ=255)                                                                            aGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt                                                       <  1:520384/140‑1 (MQ=255)                                                                            aGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt                                                       >  2:617634/1‑140 (MQ=255)                                                                            aGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt                                                       >  1:1082778/1‑140 (MQ=255)                                                                                 aaGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCaa                                                  >  1:1342982/1‑140 (MQ=255)                                                                                   ggCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAAtt                                                >  2:491497/1‑140 (MQ=255)                                                                                   ggCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAAtt                                                >  1:364881/1‑140 (MQ=255)                                                                                     ccACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCa                                              >  1:1385094/1‑140 (MQ=255)                                                                                     ccACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCa                                              >  1:1522989/1‑140 (MQ=255)                                                                                      cACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCAc                                             <  1:1338760/140‑1 (MQ=255)                                                                                        cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt                                           <  1:118558/140‑1 (MQ=255)                                                                                        cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt                                           <  2:3246/140‑1 (MQ=255)                                                                                        cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt                                           <  2:197378/140‑1 (MQ=255)                                                                                        cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt                                           >  1:97472/1‑140 (MQ=255)                                                                                        cGCCTGCAACAGCGTCATCTTGACCGAGCTGGGTAAACGACAATTCGGTTTCCGGACCGGCGGCAATGGATTCCCGATTGCCTTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTAGGCACTCACCATTCACCt                                           >  2:70813/1‑140 (MQ=255)                                                                                         gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg                                          <  2:1247998/140‑1 (MQ=255)                                                                                         gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg                                          <  2:1121796/140‑1 (MQ=255)                                                                                          ccTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGc                                         <  1:403286/140‑1 (MQ=255)                                                                                          ccTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGc                                         <  2:1096553/140‑1 (MQ=255)                                                                                           cTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCg                                        <  2:1236193/140‑1 (MQ=255)                                                                                           cTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCg                                        <  2:256315/140‑1 (MQ=255)                                                                                            tGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCGGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTCCGGCACTACCAATTCACCTGCgg                                       <  2:1379206/140‑1 (MQ=255)                                                                                                   gCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAAc                                >  2:1377513/1‑140 (MQ=255)                                                                                                     gTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTg                              <  1:998059/140‑1 (MQ=255)                                                                                                              aCCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGttt                     >  1:1433583/1‑140 (MQ=255)                                                                                                               ccGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGtttt                    <  1:191552/140‑1 (MQ=255)                                                                                                                 gAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTcc                  >  1:1134187/1‑140 (MQ=255)                                                                                                                  aGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCa                 >  1:316682/1‑140 (MQ=255)                                                                                                                    cAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACt               >  2:1378751/1‑140 (MQ=255)                                                                                                                     aGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTg              <  1:1508578/140‑1 (MQ=255)                                                                                                                             cGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGAttgt      >  1:1522301/1‑140 (MQ=255)                                                                                                                             cGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGAttgt      >  2:933438/1‑140 (MQ=255)                                                                                                                                 gaTTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCt  <  1:1056677/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCT  >  NC_000913/1541219‑1541486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑