Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	181,614	A→G	N244S (AAC→AGC)	degP →	periplasmic serine endoprotease DegP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	181,614	0	A	G	100.0%	249.1 / NA	66	N244S (AAC→AGC)	degP	periplasmic serine endoprotease DegP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/33);  total (33/33)

CTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAACGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGCCAGGTGAAACGCGGT  >  NC_000913/181478‑181750                                                                                                                                         |                                                                                                                                         cTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAAATTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCgg                                                                                                                                       <  1:462038/140‑1 (MQ=255)         gaCGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGTGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGa                                                                                                                               <  1:1461635/140‑1 (MQ=255)         gaCGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGa                                                                                                                               >  2:971982/1‑140 (MQ=255)         gaCGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGa                                                                                                                               <  2:862497/140‑1 (MQ=255)           cGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATc                                                                                                                             <  1:485988/140‑1 (MQ=255)           cGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATc                                                                                                                             <  2:150641/140‑1 (MQ=255)                aCTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTAt                                                                                                                        <  1:269115/140‑1 (MQ=255)                  ttCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCa                                                                                                                      <  1:1028103/140‑1 (MQ=255)                       ggATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACAcc                                                                                                                 <  1:236951/140‑1 (MQ=255)                         aTTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACcgc                                                                                                               <  2:1150544/140‑1 (MQ=255)                         aTTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACcgc                                                                                                               <  1:942411/140‑1 (MQ=255)                         aTTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACcgc                                                                                                               <  1:537841/140‑1 (MQ=255)                            gTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGAt                                                                                                            >  1:99930/1‑140 (MQ=255)                            gTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGAt                                                                                                            >  1:1045381/1‑140 (MQ=255)                            gTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTAGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGAt                                                                                                            <  1:1236608/140‑1 (MQ=255)                                 tGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCg                                                                                                       <  1:1460160/140‑1 (MQ=255)                                  gcgcTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGc                                                                                                      >  1:790210/1‑140 (MQ=255)                                        gggCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGa                                                                                                >  2:245509/1‑140 (MQ=255)                                        gggCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGa                                                                                                <  2:1293004/140‑1 (MQ=255)                                        gggCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGa                                                                                                >  1:290234/1‑140 (MQ=255)                                         ggCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGAc                                                                                               <  1:636908/140‑1 (MQ=255)                                          gCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGAcg                                                                                              <  1:396448/140‑1 (MQ=255)                                             tAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGAcggcg                                                                                           >  1:818100/1‑140 (MQ=255)                                                  gCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGTTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAAc                                                                                      >  1:937981/1‑140 (MQ=255)                                                  gCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAAc                                                                                      >  1:1387589/1‑140 (MQ=255)                                                  gCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAAc                                                                                      >  2:756200/1‑140 (MQ=255)                                                  gCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAAc                                                                                      >  2:376765/1‑140 (MQ=255)                                                    cTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACat                                                                                    <  1:475521/140‑1 (MQ=255)                                                    cTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACat                                                                                    <  2:1204540/140‑1 (MQ=255)                                                             gAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACatcggtatcgg                                                                           <  2:845741/140‑1 (MQ=255)                                                                 aCTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGtttt                                                                       >  1:875730/1‑140 (MQ=255)                                                                  cTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTg                                                                      <  2:99930/140‑1 (MQ=255)                                                                     cGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTa                                                                   >  1:160950/1‑140 (MQ=255)                                                                     cGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTa                                                                   <  2:1045381/140‑1 (MQ=255)                                                                      gAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTAt                                                                  <  1:426264/140‑1 (MQ=255)                                                                       aaaaCTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATc                                                                 <  1:880216/140‑1 (MQ=255)                                                                       aaaaCTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATc                                                                 <  2:1138183/140‑1 (MQ=255)                                                                           cTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGa                                                             <  1:376765/140‑1 (MQ=255)                                                                           cTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGa                                                             >  2:844357/1‑140 (MQ=255)                                                                             tCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGTGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGt                                                           >  2:897624/1‑140 (MQ=255)                                                                                 ccAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACa                                                       <  1:440113/140‑1 (MQ=255)                                                                                     aCCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGt                                                   >  2:339970/1‑140 (MQ=255)                                                                                          tGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGaaaa                                              >  2:1329542/1‑140 (MQ=255)                                                                                               cGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTg                                         >  1:787352/1‑140 (MQ=255)                                                                                               cGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTg                                         >  1:1026469/1‑140 (MQ=255)                                                                                                 aTCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGAc                                       >  1:911080/1‑140 (MQ=255)                                                                                                       cGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCa                                 <  1:844357/140‑1 (MQ=255)                                                                                                         tGGTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGa                               <  2:569659/140‑1 (MQ=255)                                                                                                           gTAACTCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGATTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGAtg                             >  2:303931/1‑140 (MQ=255)                                                                                                                tCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGa                        >  1:164922/1‑140 (MQ=255)                                                                                                                tCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGa                        >  2:213621/1‑140 (MQ=255)                                                                                                                tCCGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGGATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGa                        >  2:8641/1‑140 (MQ=255)                                                                                                                 ccGGTGGTGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGaa                       <  2:290234/140‑1 (MQ=255)                                                                                                                   ggtggtGCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATa                     >  1:1221496/1‑140 (MQ=255)                                                                                                                    gtggtgCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATAc                    >  1:295915/1‑140 (MQ=255)                                                                                                                    gtggtgCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATAc                    >  1:1373382/1‑140 (MQ=255)                                                                                                                      ggtgCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACgg                  >  2:250450/1‑140 (MQ=255)                                                                                                                       gtgtGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGc                 <  1:897624/140‑1 (MQ=255)                                                                                                                        tgCGCTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGcc                >  2:1295080/1‑140 (MQ=255)                                                                                                                          cgcTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGCCAg              >  1:372537/1‑140 (MQ=255)                                                                                                                          cgcTGGTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGCCAg              >  2:835056/1‑140 (MQ=255)                                                                                                                               gTTAACCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGCCAGGTGaa         <  2:342430/140‑1 (MQ=255)                                                                                                                                  aaCCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGCCAGGTGAAAcg      >  2:1489463/1‑140 (MQ=255)                                                                                                                                  aaCCTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGCCAGGTGAAAcg      >  2:138339/1‑140 (MQ=255)                                                                                                                                    ccTGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGCCAGGTGAAAcgcg    <  1:971982/140‑1 (MQ=255)                                                                                                                                      tGAGCGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGCCAGGTGAAACGCGGt  <  2:770600/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTGGTTAACCTGAACGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGCTATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGCCAGGTGAAACGCGGT  >  NC_000913/181478‑181750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑