Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,716,417	T→C	Y269C (TAC→TGC)	tyrS ←	tyrosine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,716,417	0	T	C	100.0%	295.2 / NA	77	Y269C (TAC→TGC)	tyrS	tyrosine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/28);  total (49/28)

CTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGT  >  NC_000913/1716280‑1716553                                                                                                                                          |                                                                                                                                         cttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCaa                                                                                                                                        >  2:907392/1‑140 (MQ=255)  ttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCaaa                                                                                                                                       >  1:306510/1‑140 (MQ=255)   tcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAAc                                                                                                                                      >  2:232828/1‑140 (MQ=255)        gTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCgg                                                                                                                                 >  2:724302/1‑140 (MQ=255)              cAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCg                                                                                                                           <  2:1215727/140‑1 (MQ=255)               aGACGAGTCCCCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGc                                                                                                                          <  2:167470/140‑1 (MQ=255)                 aCGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTTCTTTACCGCTGTTTTTATCTTCTTCTTCCAGCGCGTTGATCTCTTCAATGCTCATAAACGTTAAGAACTTCAGGAAACGGCAAACGTCGGCATCCGAAg                                                                                                                        >  2:1512257/1‑140 (MQ=255)                 aCGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAg                                                                                                                        <  1:1065064/140‑1 (MQ=255)                    aGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAgtgt                                                                                                                     <  2:1070113/140‑1 (MQ=255)                    aGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAgtgt                                                                                                                     <  1:521339/140‑1 (MQ=255)                     gTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGtt                                                                                                                    >  2:1148390/1‑140 (MQ=255)                     gTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGtt                                                                                                                    >  2:1151025/1‑140 (MQ=255)                     gTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGtt                                                                                                                    >  1:395422/1‑140 (MQ=255)                     gTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGCTTTTATCTTCTTCTTCCAGGGCGTTGATCTCCTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGtt                                                                                                                    >  2:157043/1‑140 (MQ=255)                               ccGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAAc                                                                                                          >  2:605965/1‑140 (MQ=255)                                 gCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTg                                                                                                        >  2:138169/1‑140 (MQ=255)                                      tACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGa                                                                                                   <  2:522253/140‑1 (MQ=255)                                              gggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTAc                                                                                           >  2:64052/1‑140 (MQ=255)                                               ggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACg                                                                                          >  1:1158712/1‑140 (MQ=255)                                               ggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACg                                                                                          >  1:256649/1‑140 (MQ=255)                                               ggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACg                                                                                          >  2:30623/1‑140 (MQ=255)                                               ggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACg                                                                                          >  1:1510490/1‑140 (MQ=255)                                                gcgcgcGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACgg                                                                                         >  1:17364/1‑140 (MQ=255)                                                gcgcgcGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACgg                                                                                         >  1:371432/1‑140 (MQ=255)                                                gcgcgcGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACgg                                                                                         >  1:995349/1‑140 (MQ=255)                                                gcgcgcGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACgg                                                                                         >  2:1483512/1‑140 (MQ=255)                                                gcgcgcGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACgg                                                                                         >  1:653245/1‑140 (MQ=255)                                                          cTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTttc                                                                               >  1:333414/1‑140 (MQ=255)                                                                cGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                                                         <  1:836375/140‑1 (MQ=255)                                                                cGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                                                         <  1:69015/140‑1 (MQ=255)                                                                cGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                                                         <  2:800506/140‑1 (MQ=255)                                                                cGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                                                         <  1:1397106/140‑1 (MQ=255)                                                                cGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                                                         <  1:138169/140‑1 (MQ=255)                                                                  cTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATc                                                                       >  2:240719/1‑140 (MQ=255)                                                                  cTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATc                                                                       >  2:266525/1‑140 (MQ=255)                                                                  cTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATc                                                                       >  1:385398/1‑140 (MQ=255)                                                                  cTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATc                                                                       <  1:1151025/140‑1 (MQ=255)                                                                  cTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATc                                                                       >  2:1011793/1‑140 (MQ=255)                                                                  cTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATc                                                                       <  1:1148390/140‑1 (MQ=255)                                                                             ttcttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                                            >  2:408708/1‑140 (MQ=255)                                                                               cttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgcg                                                          >  2:270562/1‑140 (MQ=255)                                                                               cttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgcg                                                          >  2:462078/1‑140 (MQ=255)                                                                                ttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgcgc                                                         <  1:1235718/140‑1 (MQ=255)                                                                                  cttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgccg                                                       >  1:1301299/1‑140 (MQ=255)                                                                                         ggCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAg                                                >  1:919606/1‑140 (MQ=255)                                                                                             ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtttt                                            <  1:907392/140‑1 (MQ=255)                                                                                              tGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTa                                           >  1:384356/1‑140 (MQ=255)                                                                                               gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTAc                                          >  1:1353864/1‑140 (MQ=255)                                                                                               gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTAc                                          >  1:1297614/1‑140 (MQ=255)                                                                                                    ctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAt                                     <  2:1199157/140‑1 (MQ=255)                                                                                                            cTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                             <  1:78339/140‑1 (MQ=255)                                                                                                            cTCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                             <  1:1514846/140‑1 (MQ=255)                                                                                                             tCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                            >  1:1098800/1‑140 (MQ=255)                                                                                                             tCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                            >  1:1383810/1‑140 (MQ=255)                                                                                                             tCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                            >  1:468965/1‑140 (MQ=255)                                                                                                             tCATAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                            <  2:1510490/140‑1 (MQ=255)                                                                                                               aTAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                             >  1:999273/1‑137 (MQ=255)                                                                                                               aTAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                             <  2:999273/137‑1 (MQ=255)                                                                                                               aTAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                          >  2:775544/1‑140 (MQ=255)                                                                                                               aTAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                          >  2:817253/1‑140 (MQ=255)                                                                                                               aTAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                          >  2:1182224/1‑140 (MQ=255)                                                                                                                tAAAGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCt                         <  2:1202191/140‑1 (MQ=255)                                                                                                                 aaaGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTg                        <  1:166916/140‑1 (MQ=255)                                                                                                                 aaaGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTg                        >  1:1335956/1‑140 (MQ=255)                                                                                                                 aaaGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTg                        <  1:462078/140‑1 (MQ=255)                                                                                                                   aGGTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCt                      <  1:724302/140‑1 (MQ=255)                                                                                                                    ggTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCtt                     <  1:389382/140‑1 (MQ=255)                                                                                                                    ggTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCtt                     <  1:270562/140‑1 (MQ=255)                                                                                                                    ggTGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCtt                     >  2:208548/1‑140 (MQ=255)                                                                                                                      tGAAGAACTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTa                   >  2:862628/1‑140 (MQ=255)                                                                                                                       gaagaaCTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAg                  >  1:1255151/1‑140 (MQ=255)                                                                                                                        aagaaCTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGt                 >  1:827977/1‑140 (MQ=255)                                                                                                                         agaaCTTCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTg                >  2:1197678/1‑140 (MQ=255)                                                                                                                               tCAGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGc          >  2:1165781/1‑140 (MQ=255)                                                                                                                                 aGGAAGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCgg        <  1:417191/140‑1 (MQ=255)                                                                                                                                     aGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACg    >  2:574387/1‑140 (MQ=255)                                                                                                                                     aGCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACg    >  1:458982/1‑140 (MQ=255)                                                                                                                                      gCGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACgg   >  1:1003158/1‑140 (MQ=255)                                                                                                                                       cGGCAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGt  <  2:371432/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGT  >  NC_000913/1716280‑1716553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑