Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,762,714	T→C	E432E (GAA→GAG)	sufB ←	Fe‑S cluster scaffold complex subunit SufB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,762,714	0	T	C	100.0%	263.8 / NA	69	E432E (GAA→GAG)	sufB	Fe‑S cluster scaffold complex subunit SufB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/44);  total (25/44)

AATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGTTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGATTTTCACTAAGCCGC  >  NC_000913/1762577‑1762850                                                                                                                                          |                                                                                                                                         aaTTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTc                                                                                                                                        <  1:872325/140‑1 (MQ=255)         cGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTgcg                                                                                                                                <  1:1161163/140‑1 (MQ=255)          ggCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTgcgc                                                                                                                               <  1:1037066/140‑1 (MQ=255)          ggCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTgcgc                                                                                                                               <  1:1358049/140‑1 (MQ=255)           gCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCa                                                                                                                              <  2:1048603/140‑1 (MQ=255)           gCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCa                                                                                                                              <  2:313317/140‑1 (MQ=255)           gCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCa                                                                                                                              <  2:1309589/140‑1 (MQ=255)           gCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCa                                                                                                                              <  1:820790/140‑1 (MQ=255)              gCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTa                                                                                                                           >  2:511472/1‑140 (MQ=255)               cTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTAt                                                                                                                          <  2:836589/140‑1 (MQ=255)                    agaACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTa                                                                                                                     <  1:361502/140‑1 (MQ=255)                    agaACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTa                                                                                                                     >  1:1203063/1‑140 (MQ=255)                    agaACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTa                                                                                                                     <  2:125819/140‑1 (MQ=255)                      aaCACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACg                                                                                                                   <  1:272286/140‑1 (MQ=255)                      aaCACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACg                                                                                                                   <  1:277992/140‑1 (MQ=255)                      aaCACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACg                                                                                                                   <  2:1260232/140‑1 (MQ=255)                       acacGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGa                                                                                                                  <  1:389690/140‑1 (MQ=255)                        cacGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGac                                                                                                                 <  2:522249/140‑1 (MQ=255)                        cacGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGac                                                                                                                 <  2:1025178/140‑1 (MQ=255)                           gTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACt                                                                                                              <  1:100851/140‑1 (MQ=255)                              tttGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCaa                                                                                                           <  2:1370131/140‑1 (MQ=255)                                      aaCCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACggg                                                                                                   <  2:1172240/140‑1 (MQ=255)                                       aCCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGTCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGa                                                                                                  >  1:1137815/1‑140 (MQ=255)                                       aCCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGa                                                                                                  >  1:257317/1‑140 (MQ=255)                                       aCCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGa                                                                                                  >  1:731590/1‑140 (MQ=255)                                                  tcatcGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGcc                                                                                       <  1:143785/140‑1 (MQ=255)                                                   catcGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGccc                                                                                      >  1:552896/1‑140 (MQ=255)                                                    atcGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGcccc                                                                                     <  2:316720/140‑1 (MQ=255)                                                      cGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCcac                                                                                   >  1:789923/1‑140 (MQ=255)                                                        agaTGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACaa                                                                                 >  2:711324/1‑140 (MQ=255)                                                          aTGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAAtt                                                                               >  1:875685/1‑140 (MQ=255)                                                              cGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAgcg                                                                           <  1:520082/140‑1 (MQ=255)                                                              cGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAgcg                                                                           <  2:1203063/140‑1 (MQ=255)                                                                  ttcttcGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCaa                                                                       >  1:133101/1‑140 (MQ=255)                                                                  ttcttcGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCaa                                                                       <  2:49839/140‑1 (MQ=255)                                                                   tcttcGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAAt                                                                      <  2:951711/140‑1 (MQ=255)                                                                   tcttcGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAAt                                                                      <  2:504724/140‑1 (MQ=255)                                                                   tcttcGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAAt                                                                      <  1:962460/140‑1 (MQ=255)                                                                        gCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCa                                                                 <  2:1090796/140‑1 (MQ=255)                                                                        gCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCa                                                                 <  2:1140324/140‑1 (MQ=255)                                                                        gCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCa                                                                 <  1:635768/140‑1 (MQ=255)                                                                        gCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCa                                                                 <  2:1068844/140‑1 (MQ=255)                                                                        gCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCa                                                                 >  1:727142/1‑140 (MQ=255)                                                                        gCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCa                                                                 <  1:1265241/140‑1 (MQ=255)                                                                        gCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCa                                                                 <  2:864238/140‑1 (MQ=255)                                                                             tCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAg                                                            >  1:726297/1‑140 (MQ=255)                                                                                  cgTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCa                                                       <  2:719052/140‑1 (MQ=255)                                                                                  cgTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCa                                                       <  1:511472/140‑1 (MQ=255)                                                                                  cgTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCa                                                       <  2:178653/140‑1 (MQ=255)                                                                                   gTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCAc                                                      >  1:708187/1‑140 (MQ=255)                                                                                      gcaggcagTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGa                                                   <  2:96414/140‑1 (MQ=255)                                                                                             gTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAAt                                            >  2:440278/1‑140 (MQ=255)                                                                                                aaaCAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTgc                                         <  2:875685/140‑1 (MQ=255)                                                                                                     gTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGCGTGAAATTGCGCGCa                                    >  2:962990/1‑140 (MQ=255)                                                                                                        gATCTTCACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTg                                 >  2:584498/1‑140 (MQ=255)                                                                                                              cACCAATACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGcc                           >  2:299608/1‑140 (MQ=255)                                                                                                                    tACGTGATGTCGTTGTCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGc                     <  2:1137815/140‑1 (MQ=255)                                                                                                                    tACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGc                     >  1:1388142/1‑140 (MQ=255)                                                                                                                    tACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGc                     >  2:394505/1‑140 (MQ=255)                                                                                                                    tACGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGc                     <  2:731590/140‑1 (MQ=255)                                                                                                                    caCGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGc                     <  2:1338012/139‑1 (MQ=255)                                                                                                                      cGTGATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCAt                   >  1:501891/1‑140 (MQ=255)                                                                                                                         gATGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGAt                <  1:514525/140‑1 (MQ=255)                                                                                                                           tGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGAttt              >  2:711142/1‑140 (MQ=255)                                                                                                                           tGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGAttt              >  1:565003/1‑140 (MQ=255)                                                                                                                           tGTCGTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGAttt              >  1:746457/1‑140 (MQ=255)                                                                                                                               gTTGCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGATTTTCAc          >  2:200628/1‑140 (MQ=255)                                                                                                                                  gCCTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGATTTTCACTaa       >  1:1389836/1‑140 (MQ=255)                                                                                                                                    cTCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGATTTTCACTAAGc     <  2:133101/140‑1 (MQ=255)                                                                                                                                     tCGTGCTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGATTTTCACTAAGcc    >  1:183110/1‑140 (MQ=255)                                                                                                                                       gtgcTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGATTTTCACTAAGCcgc  >  2:1202630/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         AATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAATACGTGATGTCGTTGCCTCGTGTTCCAGTTGCGCACTATTGTTACGACACTCAACATACGGGAAGGTATGCGCCCCACAATTAGCGCCAATCAGCATTGAGTCGCACTGAGTGAAATTGCGCGCATTGGTTGCCGTCGGCATGATTTTCACTAAGCCGC  >  NC_000913/1762577‑1762850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑