Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,904,050	C→T	R52R (CGC→CGT)	manZ →	mannose‑specific PTS enzyme IID component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,904,050	0	C	T	100.0%	214.5 / NA	63	R52R (CGC→CGT)	manZ	mannose‑specific PTS enzyme IID component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/25);  total (38/25)

ACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCA  >  NC_000913/1903913‑1904185                                                                                                                                          |                                                                                                                                        aCTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTct                                                                                                                                       >  1:116581/1‑140 (MQ=255) aCTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTct                                                                                                                                       <  1:7214/140‑1 (MQ=255)   tACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTctct                                                                                                                                     <  1:1289962/140‑1 (MQ=255)      caccGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTAcc                                                                                                                                  <  2:625494/140‑1 (MQ=255)      caccGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTAcc                                                                                                                                  <  1:371872/140‑1 (MQ=255)        ccGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCt                                                                                                                                <  1:1120701/140‑1 (MQ=255)         cGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTg                                                                                                                               >  2:1153281/1‑140 (MQ=255)             aaaaaaCTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGaa                                                                                                                           >  1:1462627/1‑140 (MQ=255)                aaaCTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGaacaa                                                                                                                        <  2:716202/140‑1 (MQ=255)                   ctcactcaAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGa                                                                                                                     <  2:1501355/140‑1 (MQ=255)                   ctcactcaAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGa                                                                                                                     <  1:1252233/140‑1 (MQ=255)                   ctcactcaAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGa                                                                                                                     >  1:371167/1‑140 (MQ=255)                            aGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTaa                                                                                                            >  1:1240674/1‑140 (MQ=255)                              tGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAAc                                                                                                          <  2:1451167/140‑1 (MQ=255)                                  atTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGc                                                                                                      >  1:708407/1‑140 (MQ=255)                                      gTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTAtt                                                                                                  >  2:820619/1‑140 (MQ=255)                                          cGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGcc                                                                                              <  1:596605/140‑1 (MQ=255)                                          cGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGcc                                                                                              <  1:1240877/140‑1 (MQ=255)                                            tCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGt                                                                                            >  2:38286/1‑140 (MQ=255)                                               tCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCAc                                                                                         >  1:1013199/1‑140 (MQ=255)                                                ccTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCAcc                                                                                        <  2:1330975/140‑1 (MQ=255)                                                    cGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGa                                                                                    <  2:375868/140‑1 (MQ=255)                                                    cGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGa                                                                                    <  1:673051/140‑1 (MQ=255)                                                    cGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGa                                                                                    >  2:928698/1‑140 (MQ=255)                                                     gTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAg                                                                                   <  1:696095/140‑1 (MQ=255)                                                        cTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTc                                                                                >  2:201024/1‑140 (MQ=255)                                                         tAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCt                                                                               >  2:1288594/1‑140 (MQ=255)                                                                ttCCAGGATTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAacac                                                                        >  1:932646/1‑140 (MQ=255)                                                                 tCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACAcc                                                                       >  1:551994/1‑140 (MQ=255)                                                                    aGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAg                                                                    <  2:1216046/140‑1 (MQ=255)                                                                       gTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCg                                                                 >  1:405563/1‑140 (MQ=255)                                                                          cATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTc                                                              >  1:486165/1‑140 (MQ=255)                                                                          cATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTc                                                              >  1:932359/1‑140 (MQ=255)                                                                          cATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTc                                                              >  1:329572/1‑140 (MQ=255)                                                                           aTGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCg                                                             >  1:166299/1‑140 (MQ=255)                                                                                  ttCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTgc                                                      >  1:614335/1‑140 (MQ=255)                                                                                  ttCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTgc                                                      >  1:452453/1‑140 (MQ=255)                                                                                           aTGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATtct                                             <  2:285389/140‑1 (MQ=255)                                                                                           aTGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATtct                                             <  2:1240674/140‑1 (MQ=255)                                                                                                ggCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCg                                        <  1:419091/140‑1 (MQ=255)                                                                                                   aCTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTaa                                     >  1:879898/1‑140 (MQ=255)                                                                                                   aCTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTaa                                     >  1:52564/1‑140 (MQ=255)                                                                                                   aCTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTaa                                     >  1:507371/1‑140 (MQ=255)                                                                                                   aCTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGACACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTaa                                     >  2:410080/1‑140 (MQ=255)                                                                                                          ttCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGgc                              <  1:1153281/140‑1 (MQ=255)                                                                                                          ttCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGgc                              <  2:932359/140‑1 (MQ=255)                                                                                                           tCTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGgcg                             <  2:116581/140‑1 (MQ=255)                                                                                                            cTGCTTCTCTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGgcgc                            <  1:201024/140‑1 (MQ=255)                                                                                                                 tctcTATGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGgaa                       >  1:915908/1‑140 (MQ=255)                                                                                                                       tGGTACCGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAg                 <  1:629910/140‑1 (MQ=255)                                                                                                                            ccGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGc            >  2:1202605/1‑140 (MQ=255)                                                                                                                            ccGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGc            >  1:813688/1‑140 (MQ=255)                                                                                                                            ccGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGc            >  2:133377/1‑140 (MQ=255)                                                                                                                            ccGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGc            >  1:1295508/1‑140 (MQ=255)                                                                                                                            ccGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGc            >  1:1225193/1‑140 (MQ=255)                                                                                                                             cGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCt           >  1:316998/1‑140 (MQ=255)                                                                                                                             cGGCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCt           >  2:409027/1‑140 (MQ=255)                                                                                                                               gCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTaa         <  2:1360986/140‑1 (MQ=255)                                                                                                                               gCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTaa         >  2:741601/1‑140 (MQ=255)                                                                                                                               gCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTaa         >  2:812759/1‑140 (MQ=255)                                                                                                                               gCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTaa         >  2:878560/1‑140 (MQ=255)                                                                                                                               gCAATTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTaa         >  1:615547/1‑140 (MQ=255)                                                                                                                                 aaTTCGTCGTCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATg       >  1:569693/1‑140 (MQ=255)                                                                                                                                      gtcgtcTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCa  <  1:1516344/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        ACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCA  >  NC_000913/1903913‑1904185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑