Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,938,454	T→C	L269S (TTG→TCG)	pykA →	pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,938,454	0	T	C	98.9%	329.3 / ‑4.0	90	L269S (TTG→TCG)	pykA	pyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (35/54);  minor base A (0/1);  total (35/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

AACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACG  >  NC_000913/1938322‑1938588                                                                                                                                     |                                                                                                                                       aaCGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCcgt                                                                                                                                 >  2:1469938/1‑140 (MQ=255)   cGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCcgtcg                                                                                                                               <  1:303223/140‑1 (MQ=255)       cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTgcg                                                                                                                           >  1:115191/1‑140 (MQ=255)       cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTgcg                                                                                                                           >  2:969127/1‑140 (MQ=255)          aaGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGt                                                                                                                        <  2:1307347/140‑1 (MQ=255)          aaGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGt                                                                                                                        <  2:924290/140‑1 (MQ=255)            gCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCa                                                                                                                      >  1:215564/1‑140 (MQ=255)             ccGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAg                                                                                                                     <  1:1112549/140‑1 (MQ=255)               gTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCt                                                                                                                   <  1:1271446/140‑1 (MQ=255)                tttGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTa                                                                                                                  >  1:1125036/1‑140 (MQ=255)                   gCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAAc                                                                                                               >  1:1203253/1‑140 (MQ=255)                   gCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAAc                                                                                                               <  2:641982/140‑1 (MQ=255)                      gCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGa                                                                                                            >  2:717282/1‑140 (MQ=255)                      gCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGa                                                                                                            <  2:259121/140‑1 (MQ=255)                         aGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGACATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCg                                                                                                         <  2:695770/140‑1 (MQ=255)                                  tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                                                                                <  1:1293332/140‑1 (MQ=255)                                  tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                                                                                <  1:146989/140‑1 (MQ=255)                                  tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                                                                                <  1:786215/140‑1 (MQ=255)                                   ggATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACgg                                                                                               <  1:755863/140‑1 (MQ=255)                                   ggATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACgg                                                                                               <  1:256237/140‑1 (MQ=255)                                     aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                                                                             >  1:1066939/1‑140 (MQ=255)                                     aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                                                                             <  2:1343747/140‑1 (MQ=255)                                     aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                                                                             <  2:438825/140‑1 (MQ=255)                                      tGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGa                                                                                            <  2:1408287/140‑1 (MQ=255)                                       gACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAc                                                                                           <  1:1169442/140‑1 (MQ=255)                                       gACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAAACACGGCGAc                                                                                           <  2:762686/140‑1 (MQ=255)                                         catcatCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAccc                                                                                         <  2:530728/140‑1 (MQ=255)                                         catcatCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAccc                                                                                         <  1:1469938/140‑1 (MQ=255)                                          atcatcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCa                                                                                        >  1:1028282/1‑140 (MQ=255)                                          atcatcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCa                                                                                        >  2:523291/1‑140 (MQ=255)                                            catcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAga                                                                                      >  2:350947/1‑140 (MQ=255)                                                 tCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGatgatg                                                                                 <  1:18508/140‑1 (MQ=255)                                                 tCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGatgatg                                                                                 <  1:82126/140‑1 (MQ=255)                                                  cGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATgg                                                                                >  2:916362/1‑140 (MQ=255)                                                    ccTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAg                                                                              <  1:211137/140‑1 (MQ=255)                                                    ccTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAg                                                                              >  2:211137/1‑140 (MQ=255)                                                      tctGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTc                                                                            <  1:1347344/140‑1 (MQ=255)                                                         gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                                                         <  2:1435560/140‑1 (MQ=255)                                                         gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                                                         <  1:1527012/140‑1 (MQ=255)                                                         gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                                                         <  2:792157/140‑1 (MQ=255)                                                         gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTAAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                                                         <  2:876692/140‑1 (MQ=255)                                                          aCGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATg                                                                        <  2:1038613/140‑1 (MQ=255)                                                             tGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGAtt                                                                     >  2:257020/1‑140 (MQ=255)                                                               gTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTAc                                                                   >  1:819199/1‑140 (MQ=255)                                                               gTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTAc                                                                   <  1:969127/140‑1 (MQ=255)                                                                 aaTGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCTGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTa                                                                 >  2:253191/1‑140 (MQ=255)                                                                  aTGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTaa                                                                >  2:385023/1‑140 (MQ=255)                                                                  aTGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTaa                                                                >  2:373205/1‑140 (MQ=255)                                                                    ggTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAcg                                                              <  1:410971/140‑2 (MQ=255)                                                                    ggTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAcc                                                              >  2:30791/1‑140 (MQ=255)                                                                       tGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGa                                                           >  2:574049/1‑140 (MQ=255)                                                                       tGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGa                                                           >  1:1371190/1‑140 (MQ=255)                                                                        gCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCTCGGCGACCCAGATGATGGAGTCAATGAt                                                                      <  1:1195728/128‑1 (MQ=255)                                                                        gCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGAt                                                                      >  2:1195728/1‑128 (MQ=255)                                                                         cACGTGGCGACCTCGGTGTGGAAATTGGCGACGCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                         >  2:929919/1‑140 (MQ=255)                                                                         cACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                         >  2:918898/1‑140 (MQ=255)                                                                         cACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                         >  2:119243/1‑140 (MQ=255)                                                                         cACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                         >  1:1253810/1‑140 (MQ=255)                                                                         cACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                         >  1:43748/1‑140 (MQ=255)                                                                          aCGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGc                                                        >  1:1090727/1‑140 (MQ=255)                                                                           cGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGcc                                                       <  2:841553/140‑1 (MQ=255)                                                                           cGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGcc                                                       <  1:717282/140‑1 (MQ=255)                                                                              ggCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAc                                                    >  1:581232/1‑140 (MQ=255)                                                                                   ccTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTg                                               <  2:821767/140‑1 (MQ=255)                                                                                      cGGTGTGGAAATTGGCGACCCGGACCTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAg                                            <  2:1125036/140‑1 (MQ=255)                                                                                                  tGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCTCGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACg                                <  2:215564/140‑1 (MQ=255)                                                                                                   ggCGACCCGGAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGt                               >  1:219607/1‑140 (MQ=255)                                                                                                           ggAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:1371190/140‑1 (MQ=255)                                                                                                           ggAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:1354866/140‑1 (MQ=255)                                                                                                           ggAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:1090727/140‑1 (MQ=255)                                                                                                           ggAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:386145/140‑1 (MQ=255)                                                                                                           ggAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:161583/140‑1 (MQ=255)                                                                                                           ggAACTGGTCGGCATTCAGAAAGCGTAGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACg                       <  2:992959/140‑1 (MQ=255)                                                                                                            gAACTGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGc                      >  2:573188/1‑140 (MQ=255)                                                                                                                tGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAGACCGAGCGGTAATCACGGCGACCCAGATTATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGtt                  <  2:323616/140‑1 (MQ=255)                                                                                                                tGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGtt                  <  1:257020/140‑1 (MQ=255)                                                                                                                tGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGtt                  <  2:442372/140‑1 (MQ=255)                                                                                                                tGGTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGtt                  <  2:794970/140‑1 (MQ=255)                                                                                                                  gTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCt                >  1:1475553/1‑140 (MQ=255)                                                                                                                  gTCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCt                >  1:414927/1‑140 (MQ=255)                                                                                                                   tCGGCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTg               >  2:1381246/1‑140 (MQ=255)                                                                                                                     gggaTTCAGAAAGCGTCGGTCCGTCGTGCGCGTCAGCTAAACCGGGGGGTAATCCCGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  2:1046261/137‑1 (MQ=255)                                                                                                                     ggCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGTCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  2:501067/140‑1 (MQ=255)                                                                                                                     ggCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  1:523291/140‑1 (MQ=255)                                                                                                                     ggCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  1:397636/140‑1 (MQ=255)                                                                                                                     ggCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  1:385023/140‑1 (MQ=255)                                                                                                                     ggCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGa             <  1:286882/140‑1 (MQ=255)                                                                                                                      gCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGAt            <  2:819199/140‑1 (MQ=255)                                                                                                                      gCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGAt            <  2:85788/140‑1 (MQ=255)                                                                                                                      gCATTCAGAAAGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGAt            >  2:609947/1‑140 (MQ=255)                                                                                                                              aaaGCGTCGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGa    <  1:916362/140‑1 (MQ=255)                                                                                                                                aGCGTCGATCCGGCGTGCGCGTCAGCTAAACCGGGCGGTAATCACGGCGACCCAGATGATGGTGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACg  <  2:1221884/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       AACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACG  >  NC_000913/1938322‑1938588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑