Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,153,048	A→G	D27D (GAT→GAC)	yegL ←	IPR002035 domain‑containing protein YegL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,153,048	0	A	G	100.0%	339.6 / NA	89	D27D (GAT→GAC)	yegL	IPR002035 domain‑containing protein YegL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/45);  total (44/45)

CACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCAATAA  >  NC_000913/2152916‑2153183                                                                                                                                     |                                                                                                                                        cACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCcagca                                                                                                                                  <  2:1525390/140‑1 (MQ=255)  aCCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCcagcag                                                                                                                                 >  1:1064658/1‑140 (MQ=255)  aCCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCcagcag                                                                                                                                 >  1:984166/1‑140 (MQ=255)   ccGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCagcagc                                                                                                                                >  1:183394/1‑140 (MQ=255)    cGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAgcagca                                                                                                                               <  1:105339/140‑1 (MQ=255)     gggCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCaa                                                                                                                              >  2:1205860/1‑140 (MQ=255)       gCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCaaaa                                                                                                                            >  1:497242/1‑140 (MQ=255)         cGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATg                                                                                                                          <  2:312815/140‑1 (MQ=255)           aaCGTCACAATACCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCa                                                                                                                        >  1:396641/1‑140 (MQ=255)            aCGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAg                                                                                                                       <  1:871451/140‑1 (MQ=255)               tCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGATACGTCCAGCAGCAAAATGCAgggg                                                                                                                    <  2:1131571/140‑1 (MQ=255)                 acaATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCa                                                                                                                  >  1:176382/1‑140 (MQ=255)                   aaTGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAAc                                                                                                                <  2:1088680/140‑1 (MQ=255)                    aTGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACg                                                                                                               <  1:521313/140‑1 (MQ=255)                    aTGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACg                                                                                                               <  1:463809/140‑1 (MQ=255)                    aTGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACg                                                                                                               <  1:1499547/140‑1 (MQ=255)                          aGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTc                                                                                                         >  2:555999/1‑140 (MQ=255)                          aGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTc                                                                                                         >  1:287814/1‑140 (MQ=255)                          aGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTc                                                                                                         >  1:1240285/1‑140 (MQ=255)                               cACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGt                                                                                                    >  1:374298/1‑140 (MQ=255)                                 ctctTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTa                                                                                                  >  2:947246/1‑140 (MQ=255)                                 ctctTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTa                                                                                                  >  1:410357/1‑140 (MQ=255)                                    tttttAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTg                                                                                               >  2:1277612/1‑140 (MQ=255)                                               aGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCt                                                                                    <  2:299874/140‑1 (MQ=255)                                               aGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCACt                                                                                    <  1:14528/140‑1 (MQ=255)                                                gCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCtg                                                                                   >  1:910873/1‑140 (MQ=255)                                                    aTCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCtggtgg                                                                               <  1:1205860/140‑1 (MQ=255)                                                    aTCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCtggtgg                                                                               <  2:497242/140‑1 (MQ=255)                                                    aTCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCtggtgg                                                                               <  2:1351752/140‑1 (MQ=255)                                                              aGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAt                                                                     >  2:123532/1‑140 (MQ=255)                                                                ttCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAttt                                                                   >  1:981491/1‑140 (MQ=255)                                                                ttCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAttt                                                                   >  2:630490/1‑140 (MQ=255)                                                                  catcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGt                                                                 >  2:1490037/1‑140 (MQ=255)                                                                   atcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGtt                                                                <  2:1011125/140‑1 (MQ=255)                                                                   atcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGtt                                                                <  2:526237/140‑1 (MQ=255)                                                                   atcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGtt                                                                <  2:565278/140‑1 (MQ=255)                                                                   atcaCGAAAGGGAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGtt                                                                >  2:378610/1‑140 (MQ=255)                                                                     caCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCg                                                              <  1:753094/140‑1 (MQ=255)                                                                       cGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCt                                                            >  1:168365/1‑140 (MQ=255)                                                                        gAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTc                                                           >  1:162088/1‑140 (MQ=255)                                                                        gAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTc                                                           >  2:1424951/1‑140 (MQ=255)                                                                        gAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTc                                                           <  1:499951/140‑1 (MQ=255)                                                                              tAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGa                                                     <  2:183394/140‑1 (MQ=255)                                                                                aCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGaaa                                                   >  1:790606/1‑140 (MQ=255)                                                                                  cAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATc                                                 <  1:828855/140‑1 (MQ=255)                                                                                  cAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATc                                                 <  2:374298/140‑1 (MQ=255)                                                                                  cAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATc                                                 <  1:439474/140‑1 (MQ=255)                                                                                     tCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCtt                                              <  1:555999/140‑1 (MQ=255)                                                                                     tCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCtt                                              >  1:593006/1‑140 (MQ=255)                                                                                               aGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGaa                                    <  2:984166/140‑1 (MQ=255)                                                                                                   cATTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTAt                                >  2:901924/1‑140 (MQ=255)                                                                                                    aTTAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATc                               >  1:1179760/1‑140 (MQ=255)                                                                                                     ttAATAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCg                              <  2:1092523/140‑1 (MQ=255)                                                                                                        aTAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAgg                           <  2:1409559/140‑1 (MQ=255)                                                                                                        aTAGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAgg                           <  2:503313/140‑1 (MQ=255)                                                                                                          aGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTc                         <  2:1064658/140‑1 (MQ=255)                                                                                                          aGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTc                         <  1:1424951/140‑1 (MQ=255)                                                                                                          aGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTc                         <  2:106416/140‑1 (MQ=255)                                                                                                          aGGTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTc                         <  1:1277612/140‑1 (MQ=255)                                                                                                           ggTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTcc                        >  2:420724/1‑140 (MQ=255)                                                                                                            gTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCg                       >  2:678995/1‑140 (MQ=255)                                                                                                            gTCTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCg                       >  2:422370/1‑140 (MQ=255)                                                                                                              cTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGt                     <  1:1490037/140‑1 (MQ=255)                                                                                                              cTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGt                     >  2:1166450/1‑140 (MQ=255)                                                                                                              cTACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGt                     >  2:568273/1‑140 (MQ=255)                                                                                                               tACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCg                              <  2:576755/130‑1 (MQ=255)                                                                                                               tACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCg                              >  1:576755/1‑130 (MQ=255)                                                                                                               tACCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTa                    >  1:795233/1‑140 (MQ=255)                                                                                                                aCCGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTAt                   >  2:537946/1‑140 (MQ=255)                                                                                                                  cGTTCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCa                 >  2:589750/1‑140 (MQ=255)                                                                                                                    ttCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATa               <  1:947246/140‑1 (MQ=255)                                                                                                                    ttCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATa               <  2:910873/140‑1 (MQ=255)                                                                                                                    ttCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATa               >  2:470899/1‑140 (MQ=255)                                                                                                                     tCATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATAc              >  1:237874/1‑140 (MQ=255)                                                                                                                      cATAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATAcc             <  2:1503624/140‑1 (MQ=255)                                                                                                                       aTAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCg            >  2:203849/1‑140 (MQ=255)                                                                                                                       aTAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCg            >  1:601713/1‑140 (MQ=255)                                                                                                                        tAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGa           <  2:981491/140‑1 (MQ=255)                                                                                                                        tAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGa           <  1:630490/140‑1 (MQ=255)                                                                                                                        tAGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGa           <  2:317322/140‑1 (MQ=255)                                                                                                                         aGAACCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGAc          <  2:176382/140‑1 (MQ=255)                                                                                                                           aaCCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTg        <  2:720069/140‑1 (MQ=255)                                                                                                                           aaCCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTg        >  1:1063725/1‑140 (MQ=255)                                                                                                                            aCCAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGc       >  2:103902/1‑140 (MQ=255)                                                                                                                             ccAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCa      <  1:305685/140‑1 (MQ=255)                                                                                                                             ccAGAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCa      <  2:1476717/140‑1 (MQ=255)                                                                                                                             ccAGAAACGTCCAGCAGCAAAATGCAGCGGCAACGTGGTTCCGGGTTACTGGCACAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCa      <  2:1363025/140‑1 (MQ=255)                                                                                                                                gAAACGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCaata   >  1:1522167/1‑140 (MQ=255)                                                                                                                                 aaaCGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCaataa  >  2:74921/1‑140 (MQ=255)                                                                                                                                 aaaCGTCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCaataa  <  1:901924/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCAATAA  >  NC_000913/2152916‑2153183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑