Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,277,320	A→G	S341G (AGC→GGC)	yejF →	putative oligopeptide ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,277,320	0	A	G	100.0%	355.8 / NA	93	S341G (AGC→GGC)	yejF	putative oligopeptide ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (51/42);  total (51/42)

GTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTG  >  NC_000913/2277185‑2277455                                                                                                                                        |                                                                                                                                        gtgGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGcat                                                                                                                                     <  2:1243986/140‑1 (MQ=255)  tgGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGcatc                                                                                                                                    <  1:451685/140‑1 (MQ=255)  tgGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGcatc                                                                                                                                    <  1:434166/140‑1 (MQ=255)     aTCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCatcatc                                                                                                                                 >  2:83510/1‑140 (MQ=255)        aTAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCttt                                                                                                                              <  1:1403285/140‑1 (MQ=255)        aTAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCACCATCttt                                                                                                                              >  2:1325456/1‑140 (MQ=255)          aaTGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGa                                                                                                                            >  1:1104950/1‑140 (MQ=255)          aaTGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGa                                                                                                                            >  1:843277/1‑140 (MQ=255)          aaTGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGa                                                                                                                            <  2:1344526/140‑1 (MQ=255)          aaTGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGa                                                                                                                            >  2:224538/1‑140 (MQ=255)          aaTGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGa                                                                                                                            >  1:1302811/1‑140 (MQ=255)               ggtggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTc                                                                                                                       >  1:627225/1‑140 (MQ=255)               ggtggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTc                                                                                                                       >  2:471791/1‑140 (MQ=255)                gcggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCACGGCGGCATCATCTTTGACGGTCa                                                                                                                      >  2:854267/3‑140 (MQ=255)                 tggtgAAAAACATCAGTTTTACGCTATGAGCGGGTGAAACACTGGGTTTAGTGGTCGAGACTGGCTCCGGGCAAAGTACGGCGGGACGGGCGATGTTTGAACTAATCAATTCTCAGGGCGGCATCATCTTTGACGGTCAg                                                                                                                     >  2:605083/1‑140 (MQ=255)                 tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGATGGTCAg                                                                                                                     >  2:179415/1‑140 (MQ=255)                 tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAg                                                                                                                     >  2:1023223/1‑140 (MQ=255)                 tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAg                                                                                                                     >  1:895549/1‑140 (MQ=255)                 tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAg                                                                                                                     >  1:648528/1‑140 (MQ=255)                 tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAg                                                                                                                     >  1:1515033/1‑140 (MQ=255)                 tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAg                                                                                                                     >  1:1500663/1‑140 (MQ=255)                 tggtgAAAAACATCAGTTTTACGCTACGAGCGGGAGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAg                                                                                                                     >  2:662298/1‑140 (MQ=255)                  ggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGc                                                                                                                    >  2:210554/1‑140 (MQ=255)                  ggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGc                                                                                                                    <  2:1395180/140‑1 (MQ=255)                   gtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGcc                                                                                                                   >  1:619044/1‑140 (MQ=255)                   gtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGcc                                                                                                                   >  1:535943/1‑140 (MQ=255)                   gtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGcc                                                                                                                   >  1:1360451/1‑140 (MQ=255)                    tgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCa                                                                                                                  >  1:692276/1‑140 (MQ=255)                    tgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCa                                                                                                                  >  1:910510/1‑140 (MQ=255)                      aaaaaCATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACt                                                                                                                >  1:573323/1‑140 (MQ=255)                        aaaCATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGc                                                                                                              <  1:514283/140‑1 (MQ=255)                             tCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAAt                                                                                                         <  2:1314944/140‑1 (MQ=255)                             tCAGTTTTACGCTACGAGCGGGAGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAAt                                                                                                         >  1:402164/1‑140 (MQ=255)                                    tACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATc                                                                                                  <  2:812905/140‑1 (MQ=255)                                        cTACGAGCGGGTGAAACACTGGGTTTAGGGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGGATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATcgccg                                                                                              <  2:1330348/140‑1 (MQ=255)                                         tACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATcgccgc                                                                                             <  1:638137/140‑1 (MQ=255)                                         tACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATcgccgc                                                                                             <  2:799223/140‑1 (MQ=255)                                         tACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATcgccgc                                                                                             <  1:83510/140‑1 (MQ=255)                                           cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCa                                                                                           <  1:164010/140‑1 (MQ=255)                                            gAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGATGGTCAGCCACTGCAAAATTTAAATCGCCGCCAg                                                                                          <  1:179415/140‑1 (MQ=255)                                            gAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAg                                                                                          <  2:1515033/140‑1 (MQ=255)                                               cGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTg                                                                                       <  1:1149771/140‑1 (MQ=255)                                                 ggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAgttgtt                                                                                     >  2:407028/1‑140 (MQ=255)                                                 ggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGtt                                                                                     <  1:1516487/140‑1 (MQ=255)                                                 ggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGtt                                                                                     <  2:535943/140‑1 (MQ=255)                                                     aaaCACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCt                                                                                 >  2:838998/1‑140 (MQ=255)                                                     aaaCACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCt                                                                                 <  1:1188044/140‑1 (MQ=255)                                                       acacTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTAACCTAt                                                                               >  2:1102049/1‑140 (MQ=255)                                                            gggTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTc                                                                          >  1:1174851/1‑140 (MQ=255)                                                            gggTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTc                                                                          >  1:377518/1‑140 (MQ=255)                                                             ggTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCa                                                                         >  1:1261148/1‑140 (MQ=255)                                                              gTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCAt                                                                        <  1:210554/140‑1 (MQ=255)                                                                ttAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCg                                                                      <  2:465809/140‑1 (MQ=255)                                                                 tAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGc                                                                     >  1:775965/1‑140 (MQ=255)                                                                      ggCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCa                                                                <  1:224538/140‑1 (MQ=255)                                                                       gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAg                                                               >  1:1047540/1‑140 (MQ=255)                                                                       gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAg                                                               >  2:1047305/1‑140 (MQ=255)                                                                       gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAg                                                               >  1:965038/1‑140 (MQ=255)                                                                        cGAGTCCGGTTCCGGGAAAAGTACGCCGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAgg                                                              <  1:229555/140‑1 (MQ=255)                                                                                 ttCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTc                                                     <  1:838998/140‑1 (MQ=255)                                                                                 ttCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTc                                                     <  1:946625/140‑1 (MQ=255)                                                                                   ccGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGTTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAg                                                   <  1:407028/140‑1 (MQ=255)                                                                                   ccGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAg                                                   <  1:645317/140‑1 (MQ=255)                                                                                      ggAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGAt                                                >  2:1200614/1‑140 (MQ=255)                                                                                      ggAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGAt                                                >  1:479635/1‑140 (MQ=255)                                                                                           aGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCaaa                                           >  1:1229312/1‑140 (MQ=255)                                                                                             tACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAAct                                         >  2:240443/1‑140 (MQ=255)                                                                                              acgacgGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAActc                                        <  2:1202285/140‑1 (MQ=255)                                                                                                    ggtcTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACAGTCATTTTAAGATAAATGTAAATAGCCGCCAAATGTTCCCTATTCGTCGTCGCATTCTGGTGGTATTTCAGGATCTAAACTCCTCGCt                                  <  2:928007/137‑1 (MQ=255)                                                                                                    ggACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCt                                  <  2:895549/140‑1 (MQ=255)                                                                                                    ggACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCt                                  <  1:1325456/140‑1 (MQ=255)                                                                                                    ggACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCt                                  <  1:1494861/140‑1 (MQ=255)                                                                                                    ggACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCt                                  <  2:1512054/140‑1 (MQ=255)                                                                                                    ggACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCt                                  <  1:662298/140‑1 (MQ=255)                                                                                                    ggACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCt                                  <  2:910510/140‑1 (MQ=255)                                                                                                      aCTGGCGCTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCa                                >  2:858637/1‑140 (MQ=255)                                                                                                          gcgcTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAAccc                            >  2:847671/1‑140 (MQ=255)                                                                                                           cgcTGCTGCGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCa                           >  1:561043/1‑140 (MQ=255)                                                                                                               gctgcGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGAc                       >  1:1279331/1‑140 (MQ=255)                                                                                                               gctgcGACTGATTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGAc                       >  1:723167/1‑140 (MQ=255)                                                                                                                         aTTAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGtttt             <  2:479635/140‑1 (MQ=255)                                                                                                                          ttAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAACCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTg            >  2:7379/1‑140 (MQ=255)                                                                                                                           tAATTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGc           <  1:428509/140‑1 (MQ=255)                                                                                                                            aaTTCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCa          <  2:1047540/140‑1 (MQ=255)                                                                                                                              ttCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGa        <  2:619044/140‑1 (MQ=255)                                                                                                                              ttCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGa        <  1:471791/140‑1 (MQ=255)                                                                                                                              ttCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGa        <  1:1047305/140‑1 (MQ=255)                                                                                                                              ttCTCAGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGa        <  2:1261148/140‑1 (MQ=255)                                                                                                                                   aGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGattatt   >  1:1336254/1‑140 (MQ=255)                                                                                                                                   aGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGattatt   >  1:1276006/1‑140 (MQ=255)                                                                                                                                   aGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGattatt   >  2:394545/1‑140 (MQ=255)                                                                                                                                   aGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGattatt   >  1:73842/1‑140 (MQ=255)                                                                                                                                   aGGGCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGattatt   >  1:94750/1‑140 (MQ=255)                                                                                                                                    gggCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTg  >  1:59289/1‑140 (MQ=255)                                                                                                                                    gggCGGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTg  >  1:869228/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTG  >  NC_000913/2277185‑2277455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑