Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,277,598	T→C	R433R (CGT→CGC)	yejF →	putative oligopeptide ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,277,598	0	T	C	100.0%	278.4 / NA	74	R433R (CGT→CGC)	yejF	putative oligopeptide ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/31);  total (43/31)

TTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGCCT  >  NC_000913/2277471‑2277734                                                                                                                                |                                                                                                                                         ttCACCAGCCGACGCTTTCTGTCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGcc                                                                                                                              <  2:1424399/140‑1 (MQ=255)  tCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACATGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCa                                                                                                                             >  2:1252019/1‑140 (MQ=255)  tCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCa                                                                                                                             <  2:1417549/140‑1 (MQ=255)          cGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTa                                                                                                                     <  2:547342/140‑1 (MQ=255)          cGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTa                                                                                                                     <  2:471266/140‑1 (MQ=255)          cGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTa                                                                                                                     <  1:1352094/140‑1 (MQ=255)             cGCTTTCTGCCGCACAACGCGAACAACAAGCGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAAtt                                                                                                                  >  2:1209699/1‑140 (MQ=255)                  tCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTaa                                                                                                             >  1:672855/1‑140 (MQ=255)                  tCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTaa                                                                                                             >  1:424692/1‑140 (MQ=255)                    tGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGc                                                                                                           <  2:672268/140‑1 (MQ=255)                    tGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGc                                                                                                           <  1:1310147/140‑1 (MQ=255)                      ccGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGccc                                                                                                         <  1:620371/140‑1 (MQ=255)                      ccGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGccc                                                                                                         <  1:522948/140‑1 (MQ=255)                       cGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCt                                                                                                        >  2:1260684/1‑140 (MQ=255)                         cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                      <  1:406927/140‑1 (MQ=255)                          acaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGc                                                                                                     <  1:199670/140‑1 (MQ=255)                              cgcgAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGAt                                                                                                 >  2:713849/1‑140 (MQ=255)                                 gAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGTGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCAt                                                                                              <  2:1174851/140‑1 (MQ=255)                                        aaGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTtgat                                                                                       <  1:311197/140‑1 (MQ=255)                                        aaGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTtgat                                                                                       >  2:422249/1‑140 (MQ=255)                                           tGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGaa                                                                                    <  1:1333131/140‑1 (MQ=255)                                             aTAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAAcc                                                                                  >  2:1058458/1‑140 (MQ=255)                                              tAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCg                                                                                 <  1:718032/140‑1 (MQ=255)                                               aGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGa                                                                                >  2:766510/1‑140 (MQ=255)                                                     gATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatcat                                                                          >  1:1403979/1‑140 (MQ=255)                                                      aTGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACatcatc                                                                         >  2:241165/1‑140 (MQ=255)                                                         aaTGAAGTGGTATTAGATCCTGAAACCCGCGCCCGTTATCCGACGGAGTTCTCTGGTGGTTATCTACAATGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGTTGATCATACTGGATGAACCGACATCATCACt                                                                      <  2:1336254/139‑1 (MQ=255)                                                           tGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCg                                                                    >  2:941096/1‑140 (MQ=255)                                                               gTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaa                                                                <  2:270739/140‑1 (MQ=255)                                                                 gggATTAGATCCTGAAACATGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaaaa                                                              <  1:1252019/140‑1 (MQ=255)                                                                 gggATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaaaa                                                              >  2:1308361/1‑140 (MQ=255)                                                                  ggATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAAc                                                             >  1:1283064/1‑140 (MQ=255)                                                                    aTTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACgg                                                           >  2:238860/1‑140 (MQ=255)                                                                    aTTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACgg                                                           >  1:1252443/1‑140 (MQ=255)                                                                      tAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTa                                                         >  1:659011/1‑140 (MQ=255)                                                                       aGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTAc                                                        <  2:161504/140‑1 (MQ=255)                                                                        gATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACa                                                       <  2:1198366/140‑1 (MQ=255)                                                                        gATCCTGAAACACGCCACCGTTATCCGGCGGAGGTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACa                                                       >  2:1477312/1‑140 (MQ=255)                                                                          tCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCAGACTTGATGAACCGACATCATCACTCGACAAAACGGAACacg                                                     >  1:1361499/1‑138 (MQ=255)                                                                             tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                                  >  2:1019198/1‑140 (MQ=255)                                                                             tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                                  >  2:664994/1‑140 (MQ=255)                                                                             tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                                  >  1:1121861/1‑140 (MQ=255)                                                                             tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                                  >  2:896054/1‑140 (MQ=255)                                                                              gAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCa                                                 >  1:240186/1‑140 (MQ=255)                                                                                   acGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAatat                                            >  2:587787/1‑140 (MQ=255)                                                                                    cGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATAtt                                           >  2:748058/1‑140 (MQ=255)                                                                                    cGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATAtt                                           >  1:797842/1‑140 (MQ=255)                                                                                     gCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTg                                          >  1:530041/1‑140 (MQ=255)                                                                                     gCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTg                                          >  2:86496/1‑140 (MQ=255)                                                                                      ccACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGa                                         <  1:422249/140‑1 (MQ=255)                                                                                        aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                       <  1:622656/140‑1 (MQ=255)                                                                                        aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                       <  1:1331208/140‑1 (MQ=255)                                                                                        aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                       <  1:769304/140‑1 (MQ=255)                                                                                         ccGTTATCCGGCGGCGTTCTCTGGTGGACAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCTCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGc                                      >  2:845291/1‑140 (MQ=255)                                                                                         ccGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGCACAGGCGCAAATATTGACGc                                      >  2:1466439/1‑140 (MQ=255)                                                                                            ttATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTAt                                   >  2:935479/1‑140 (MQ=255)                                                                                            ttATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTAt                                   >  2:1076037/1‑140 (MQ=255)                                                                                               tCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAAACGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGa                                >  1:930874/1‑140 (MQ=255)                                                                                                ccGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGaa                               <  2:530041/140‑1 (MQ=255)                                                                                                    cggAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCa                           >  1:312726/1‑140 (MQ=255)                                                                                                    cggAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCa                           >  2:682860/1‑140 (MQ=255)                                                                                                       aGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCAATCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTg                        >  1:845357/1‑140 (MQ=255)                                                                                                            tctGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACGGGCGCAAATATTGACGCTATTGAAATCATTGcaaca                   <  2:356957/140‑1 (MQ=255)                                                                                                                 tggtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGc              <  2:71555/140‑1 (MQ=255)                                                                                                                   gtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCAt            >  2:1445652/1‑140 (MQ=255)                                                                                                                   gtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCAt            >  2:84767/1‑140 (MQ=255)                                                                                                                   gtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCAt            >  1:125617/1‑140 (MQ=255)                                                                                                                   gtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCAt            >  1:207498/1‑140 (MQ=255)                                                                                                                   gtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCAt            >  1:131636/1‑140 (MQ=255)                                                                                                                      aGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa         >  2:1319090/1‑140 (MQ=255)                                                                                                                      aGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa         <  1:1260684/140‑1 (MQ=255)                                                                                                                      aGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGAC‑AAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCacc        >  1:609801/1‑138 (MQ=255)                                                                                                                           cAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTTACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGc    <  2:672855/140‑1 (MQ=255)                                                                                                                           cAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGc    <  2:424692/140‑1 (MQ=255)                                                                                                                            aaCGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGcc   <  1:833426/140‑1 (MQ=255)                                                                                                                             aCGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGCCt  <  1:1058458/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                         TTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGCCT  >  NC_000913/2277471‑2277734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑