BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	280,365	G→A	intergenic (‑490/+20)	insA‑2 ← / ← yagB	IS1 protein InsA/orphan antitoxin YagB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	280,365	0	G	A	100.0%	380.0 / NA	111	intergenic (‑490/+20)	insA‑2/yagB	IS1 protein InsA/orphan antitoxin YagB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (46/65); total (46/65)

Only 100 of 114 total aligned reads displayed.

ACTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGA  >  NC_000913/280227‑280500

aCTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGAT‑TCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACa                                                                                                                                       >  1:697176/1‑140 (MQ=255)

   tcttTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACAcc                                                                                                                                     <  1:68979/140‑1 (MQ=255)

    cttTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACGCct                                                                                                                                    <  2:207335/140‑1 (MQ=255)

    cttTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACct                                                                                                                                    <  2:1249607/140‑1 (MQ=255)

       tATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACctctc                                                                                                                                 >  1:1521334/1‑140 (MQ=255)

         tCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCtt                                                                                                                               >  1:616740/1‑140 (MQ=255)

            aGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTaa                                                                                                                            >  2:753854/1‑140 (MQ=255)

             ggCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAg                                                                                                                           <  2:1095343/140‑1 (MQ=255)

             ggCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAg                                                                                                                           <  2:442449/140‑1 (MQ=255)

              gCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGc                                                                                                                          >  2:1060489/1‑140 (MQ=255)

              gCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGc                                                                                                                          >  2:475452/1‑140 (MQ=255)

              gCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGc                                                                                                                          >  1:53955/1‑140 (MQ=255)

              gCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGc                                                                                                                          >  1:55983/1‑140 (MQ=255)

              gCATAAAGGCTGTGGGTGGTGATATCGAGACGTGATGCAACGCTGGCAACAGAATAACCGAGATCACCAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGc                                                                                                                          >  1:252426/1‑140 (MQ=255)

                aTAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCa                                                                                                                        <  1:597795/140‑1 (MQ=255)

                  aaaGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCAtt                                                                                                                      >  2:1040880/1‑140 (MQ=255)

                   aaGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCAttt                                                                                                                     >  1:200839/1‑140 (MQ=255)

                       cTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTaaa                                                                                                                 <  2:1171907/140‑1 (MQ=255)

                       cTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTaaa                                                                                                                 <  1:1375829/140‑1 (MQ=255)

                        tgtgGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAAt                                                                                                                <  2:632923/140‑1 (MQ=255)

                        tgtgGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAAt                                                                                                                >  2:404013/1‑140 (MQ=255)

                        tgtgGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAAt                                                                                                                >  2:48347/1‑140 (MQ=255)

                         gtgggtggTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATg                                                                                                               >  1:315664/1‑140 (MQ=255)

                           gggtggTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGAc                                                                                                             >  2:1184904/1‑140 (MQ=255)

                                gtgATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGa                                                                                                        >  2:182938/1‑140 (MQ=255)

                                gtgATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGa                                                                                                        >  2:831456/1‑140 (MQ=255)

                                    tatCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgtg                                                                                                    <  2:880918/140‑1 (MQ=255)

                                    tatCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgtg                                                                                                    >  1:287918/1‑140 (MQ=255)

                                    tatCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgtg                                                                                                    <  1:1264936/140‑1 (MQ=255)

                                     atCGCGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgtgt                                                                                                   <  1:676633/140‑1 (MQ=255)

                                     atCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgtgt                                                                                                   <  1:1052138/140‑1 (MQ=255)

                                      tCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTc                                                                                                  >  2:834944/1‑140 (MQ=255)

                                       cGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCt                                                                                                 >  1:864583/1‑140 (MQ=255)

                                              tgtTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAAc                                                                                          >  2:1251742/1‑140 (MQ=255)

                                               gtTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAAcc                                                                                         >  1:380724/1‑140 (MQ=255)

                                               gtTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAAcc                                                                                         >  2:291321/1‑140 (MQ=255)

                                                ttGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAAccc                                                                                        >  2:1091320/1‑140 (MQ=255)

                                                ttGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAAccc                                                                                        >  1:987808/1‑140 (MQ=255)

                                                        cTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGAt                                                                                >  2:1473003/1‑140 (MQ=255)

                                                        cTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGAt                                                                                >  1:936365/1‑140 (MQ=255)

                                                         tGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGAtt                                                                               >  2:1171175/1‑140 (MQ=255)

                                                           gCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCa                                                                             >  1:48676/1‑140 (MQ=255)

                                                                aGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAACTGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAAtt                                                                        <  1:1060489/140‑1 (MQ=255)

                                                                aGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAAtt                                                                        <  2:625124/140‑1 (MQ=255)

                                                                aGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAAtt                                                                        <  1:341509/140‑1 (MQ=255)

                                                                aGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAAtt                                                                        <  1:730503/140‑1 (MQ=255)

                                                                 gAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTg                                                                       <  1:142066/140‑1 (MQ=255)

                                                                 gAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTg                                                                       <  1:1362227/140‑1 (MQ=255)

                                                                 gAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTg                                                                       <  2:608677/140‑1 (MQ=255)

                                                                   aTAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCg                                                                     <  1:963329/140‑1 (MQ=255)

                                                                   aTAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCg                                                                     <  1:1480137/140‑1 (MQ=255)

                                                                   aTAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCg                                                                     <  1:404013/140‑1 (MQ=255)

                                                                    tAACCGAGTTCAACAGCCTGTTTGGCCGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACGCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGa                                                                    <  2:1071128/140‑1 (MQ=255)

                                                                    tAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGa                                                                    <  2:200839/140‑1 (MQ=255)

                                                                    tAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGa                                                                    <  2:1412077/140‑1 (MQ=255)

                                                                    tAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGa                                                                    <  2:310979/140‑1 (MQ=255)

                                                                     aaCCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGat                                                                   <  1:411405/140‑1 (MQ=255)

                                                                     aaCCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGat                                                                   <  1:501658/140‑1 (MQ=255)

                                                                     aaCCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGat                                                                   >  2:692190/1‑140 (MQ=255)

                                                                     aaCCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGat                                                                   <  1:182938/140‑1 (MQ=255)

                                                                     aaCCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGat                                                                   <  2:1371541/140‑1 (MQ=255)

                                                                      aCCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGata                                                                  <  2:53955/140‑1 (MQ=255)

                                                                       ccGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGatat                                                                 <  2:362138/140‑1 (MQ=255)

                                                                       ccGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGatat                                                                 <  1:1184904/140‑1 (MQ=255)

                                                                           gaTCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATatac                                                             <  2:55983/140‑1 (MQ=255)

                                                                           gaTCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATatac                                                             <  1:1512410/140‑1 (MQ=255)

                                                                           gaTCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATatac                                                             <  2:697176/140‑1 (MQ=255)

                                                                                  aacCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCa                                                      >  2:871139/1‑140 (MQ=255)

                                                                                      tGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGAc                                                  >  2:742772/1‑140 (MQ=255)

                                                                                        tttGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTg                                                >  1:401436/1‑140 (MQ=255)

                                                                                          tGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGcc                                              <  1:831456/140‑1 (MQ=255)

                                                                                                 ttCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTa                                       <  2:48676/140‑1 (MQ=255)

                                                                                                          aaaCTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCa                              <  1:647566/140‑1 (MQ=255)

                                                                                                           aaCTCTTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTACACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCAc                             >  1:214624/1‑140 (MQ=255)

                                                                                                               ctTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGt                         <  1:1171175/140‑1 (MQ=255)

                                                                                                               ctTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGt                         <  1:1040880/140‑1 (MQ=255)

                                                                                                               ctTCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGt                         <  1:1165376/140‑1 (MQ=255)

                                                                                                                ttCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTc                        >  1:1159020/1‑140 (MQ=255)

                                                                                                                 tCGGGATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCa                       >  1:991955/1‑140 (MQ=255)

                                                                                                                    ggATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGcc                    <  2:496511/140‑1 (MQ=255)

                                                                                                                    ggATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGcc                    <  2:936365/140‑1 (MQ=255)

                                                                                                                    ggATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGcc                    <  2:237989/140‑1 (MQ=255)

                                                                                                                    ggATAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGcc                    <  2:4016/140‑1 (MQ=255)

                                                                                                                      aTAACGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCg                  <  1:34129/140‑1 (MQ=255)

                                                                                                                          cGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTAc              >  2:354952/1‑140 (MQ=255)

                                                                                                                          cGCTTACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTAc              >  1:175437/1‑140 (MQ=255)

                                                                                                                              tACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTa          <  1:834944/140‑1 (MQ=255)

                                                                                                                              tACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTa          <  1:692190/140‑1 (MQ=255)

                                                                                                                              tACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTa          <  1:823563/140‑1 (MQ=255)

                                                                                                                              tACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTa          <  2:864583/140‑1 (MQ=255)

                                                                                                                              tACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTa          <  1:1091320/140‑1 (MQ=255)

                                                                                                                              tACCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTa          <  1:1513287/140‑1 (MQ=255)

                                                                                                                               aCCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGTAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTAc         >  2:846634/1‑140 (MQ=255)

                                                                                                                               aCCGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTAc         >  1:584393/1‑140 (MQ=255)

                                                                                                                                ccGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACa        <  1:48347/140‑1 (MQ=255)

                                                                                                                                ccGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACa        <  1:829095/140‑1 (MQ=255)

                                                                                                                                ccGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACa        <  2:287918/140‑1 (MQ=255)

                                                                                                                                ccGCTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACa        <  2:315664/140‑1 (MQ=255)

                                                                                                                                   cTCATGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAgtg     >  2:77627/1‑140 (MQ=255)

                                                                                                                                      aTGGACACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGa  >  2:1081232/1‑140 (MQ=255)

ACTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGA  >  NC_000913/280227‑280500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`