Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,309,798	A→G	V203A (GTG→GCG)	ada ←	DNA‑binding transcriptional dual regulator/DNA repair protein Ada

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,309,798	0	A	G	100.0%	196.6 / NA	54	V203A (GTG→GCG)	ada	DNA‑binding transcriptional dual regulator/DNA repair protein Ada
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/24);  total (30/24)

CATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGG  >  NC_000913/2309670‑2309928                                                                                                                                 |                                                                                                                                   caTGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGa                                                                                                                         >  1:696587/1‑140 (MQ=255)  aTGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGAc                                                                                                                        >  1:236617/1‑140 (MQ=255)   tGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGAcc                                                                                                                       <  2:1233445/140‑1 (MQ=255)   tGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGAcc                                                                                                                       >  1:258953/1‑140 (MQ=255)   tGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGAcc                                                                                                                       <  2:196785/140‑1 (MQ=255)       gCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGc                                                                                                                   >  1:354688/1‑140 (MQ=255)        cTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCt                                                                                                                  <  2:154391/140‑1 (MQ=255)            aaCATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTcaca                                                                                                              <  2:1267070/140‑1 (MQ=255)               aTCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATc                                                                                                           >  1:855973/1‑140 (MQ=255)                tCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATca                                                                                                          >  1:1341259/1‑140 (MQ=255)                tCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATca                                                                                                          >  1:742852/1‑140 (MQ=255)                  aGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATcagc                                                                                                        >  1:388936/1‑140 (MQ=255)                  aGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATcagc                                                                                                        <  2:170657/140‑1 (MQ=255)                  aGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCCGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATcagc                                                                                                        >  2:460307/1‑140 (MQ=255)                   gATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATcagcc                                                                                                       <  2:768534/140‑1 (MQ=255)                    aTCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATcagcca                                                                                                      <  2:121485/140‑1 (MQ=255)                                 ttGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCAc                                                                                         <  2:351010/140‑1 (MQ=255)                                  tGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCAcc                                                                                        >  1:927280/1‑140 (MQ=255)                                   gTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCg                                                                                       >  1:368651/1‑140 (MQ=255)                                     cGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGcc                                                                                     <  2:1341259/140‑1 (MQ=255)                                                       gctgcAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGa                                                                   >  1:926490/1‑140 (MQ=255)                                                        ctgcAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGAc                                                                  >  1:302741/1‑140 (MQ=255)                                                            aaCTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGaa                                                              <  2:390148/140‑1 (MQ=255)                                                                cGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGt                                                          >  1:820621/1‑140 (MQ=255)                                                                 gCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGtt                                                         >  2:24994/1‑140 (MQ=255)                                                                    gATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAg                                                      <  2:155404/140‑1 (MQ=255)                                                                          tgtCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCa                                                >  2:96606/1‑140 (MQ=255)                                                                              gcgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGcc                                            <  2:170669/140‑1 (MQ=255)                                                                               cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  1:862027/1‑140 (MQ=255)                                                                               cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  2:1525320/1‑140 (MQ=255)                                                                               cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  2:844747/1‑140 (MQ=255)                                                                               cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  2:676998/1‑140 (MQ=255)                                                                               cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           <  1:286693/140‑1 (MQ=255)                                                                               cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  2:1194825/1‑140 (MQ=255)                                                                               cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  1:189858/1‑140 (MQ=255)                                                                                  catcatCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGc                                        <  2:696587/140‑1 (MQ=255)                                                                                   atcatcGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCg                                       >  2:1525941/1‑140 (MQ=255)                                                                                          cccAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTc                                >  1:969568/1‑140 (MQ=255)                                                                                            cAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAg                              <  2:855973/140‑1 (MQ=255)                                                                                              gCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCt                            <  2:368651/140‑1 (MQ=255)                                                                                                 atatCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTg                         <  2:258953/140‑1 (MQ=255)                                                                                                  tatCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGACAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGc                        <  1:688917/140‑1 (MQ=255)                                                                                                       cgcAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAg                   >  2:701134/1‑140 (MQ=255)                                                                                                       cgcAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAg                   >  1:12450/1‑140 (MQ=255)                                                                                                        gcAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGt                  >  1:1324073/1‑140 (MQ=255)                                                                                                         cAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTa                 >  1:243245/1‑140 (MQ=255)                                                                                                         cAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTa                 >  2:854121/1‑140 (MQ=255)                                                                                                            aTCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAAct              >  2:959161/1‑140 (MQ=255)                                                                                                                cccGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAActgctg          <  1:1525941/140‑1 (MQ=255)                                                                                                                cccGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAActgctg          <  1:737864/140‑1 (MQ=255)                                                                                                                cccGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAActgctg          <  1:96606/140‑1 (MQ=255)                                                                                                                cccGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAActgctg          <  2:926490/140‑1 (MQ=255)                                                                                                                   gctcgctTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAActgctgctg       <  1:145630/140‑1 (MQ=255)                                                                                                                       gctTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCgg   <  2:388936/140‑1 (MQ=255)                                                                                                                        ctTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCggg  >  1:64219/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                   CATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGG  >  NC_000913/2309670‑2309928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑