Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,456,408	G→A	S135S (AGC→AGT)	sixA ←	phosphohistidine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,456,408	0	G	A	100.0%	321.0 / NA	96	S135S (AGC→AGT)	sixA	phosphohistidine phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/61);  total (35/61)

ATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCAAAT  >  NC_000913/2456275‑2456541                                                                                                                                      |                                                                                                                                      atgaGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCa                                                                                                                                 <  2:476578/140‑1 (MQ=255)        aTTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCg                                                                                                                          <  2:443677/140‑1 (MQ=255)        aTTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCg                                                                                                                          <  2:1494087/140‑1 (MQ=255)         ttATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGa                                                                                                                         <  2:1008368/140‑1 (MQ=255)          tATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAg                                                                                                                        <  2:671140/140‑1 (MQ=255)          tATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAg                                                                                                                        <  1:1427458/140‑1 (MQ=255)          tATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAg                                                                                                                        <  1:1506600/140‑1 (MQ=255)           aTGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAgg                                                                                                                       >  2:869512/1‑140 (MQ=255)             gCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAggtg                                                                                                                     <  2:880694/140‑1 (MQ=255)             gCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAggtg                                                                                                                     >  2:1017057/1‑140 (MQ=255)                  aTGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTaaa                                                                                                                >  2:804964/1‑140 (MQ=255)                   tGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAAc                                                                                                               >  2:696936/1‑140 (MQ=255)                   tGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAAc                                                                                                               >  2:1474958/1‑140 (MQ=255)                   tGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAAc                                                                                                               >  1:1501952/1‑140 (MQ=255)                      aTCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATa                                                                                                            >  2:1429183/1‑140 (MQ=255)                          aCCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAggcg                                                                                                        >  1:796026/1‑140 (MQ=255)                           ccTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAggcgg                                                                                                       >  2:1175011/1‑140 (MQ=255)                               aTTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGtt                                                                                                   >  2:538558/1‑140 (MQ=255)                               aTTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGACAATGGCCGAGGTGGTAAACATAGGCGGCGtt                                                                                                   >  2:1392899/1‑140 (MQ=255)                                   ggCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGc                                                                                               <  2:292620/140‑1 (MQ=255)                                   ggCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGc                                                                                               >  2:1324083/1‑140 (MQ=255)                                   ggCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGc                                                                                               <  1:594290/140‑1 (MQ=255)                                    gCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGcc                                                                                              <  1:843862/140‑1 (MQ=255)                                    gCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGcc                                                                                              <  1:1238943/140‑1 (MQ=255)                                     cATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGccc                                                                                             >  2:237929/1‑140 (MQ=255)                                     cATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGccc                                                                                             <  2:1457021/140‑1 (MQ=255)                                     cATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGccc                                                                                             <  2:47943/140‑1 (MQ=255)                                     cATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGccc                                                                                             >  2:552264/1‑140 (MQ=255)                                     cATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGccc                                                                                             >  2:1280268/1‑140 (MQ=255)                                        aaTTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGa                                                                                          <  1:1006674/140‑1 (MQ=255)                                         aTTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGAc                                                                                         >  2:709976/1‑140 (MQ=255)                                          tttGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACa                                                                                        >  2:1217436/1‑140 (MQ=255)                                          tttGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACa                                                                                        <  1:223584/140‑1 (MQ=255)                                             ggAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATaa                                                                                     >  1:1323289/1‑140 (MQ=255)                                               aaCTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACt                                                                                   <  2:1211876/140‑1 (MQ=255)                                                aCTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTc                                                                                  <  1:362247/140‑1 (MQ=255)                                                   catcaGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGc                                                                               <  2:1111860/140‑1 (MQ=255)                                                   catcaGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGc                                                                               >  1:376475/1‑140 (MQ=255)                                                   catcaGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGc                                                                               <  2:1358712/140‑1 (MQ=255)                                                    atcaGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCa                                                                              >  1:946135/1‑140 (MQ=255)                                                      caGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTTGGCAAc                                                                            >  1:95943/1‑140 (MQ=255)                                                       aGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAAcc                                                                           <  2:768164/140‑1 (MQ=255)                                                       aGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAAcc                                                                           <  2:1351286/140‑1 (MQ=255)                                                       aGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAAcc                                                                           <  2:763776/140‑1 (MQ=255)                                                          tAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGa                                                                        <  1:1175011/140‑1 (MQ=255)                                                             cTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATAc                                                                     >  2:216639/1‑140 (MQ=255)                                                              ttttGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATAcc                                                                    >  2:324802/1‑140 (MQ=255)                                                               tttGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATAccc                                                                   <  2:606683/140‑1 (MQ=255)                                                                 tGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGa                                                                 <  1:478918/140‑1 (MQ=255)                                                                    cATCTTCAGATTACACGGGCTCATCTGCCAGTT‑AATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTaa                                                             <  2:1449645/140‑1 (MQ=255)                                                                     aTCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTaa                                                             <  1:278745/140‑1 (MQ=255)                                                                       cTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACg                                                           <  1:1017057/140‑1 (MQ=255)                                                                         tCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGt                                                         <  1:289598/140‑1 (MQ=255)                                                                         tCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGt                                                         <  1:1164733/140‑1 (MQ=255)                                                                         tCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGt                                                         <  1:552264/140‑1 (MQ=255)                                                                          cAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTa                                                        >  2:37574/1‑140 (MQ=255)                                                                             aTTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGt                                                     <  2:107833/140‑1 (MQ=255)                                                                             aTTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGt                                                     >  1:1225499/1‑140 (MQ=255)                                                                             aTTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGt                                                     <  2:1489420/140‑1 (MQ=255)                                                                               tACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTga                                                   >  2:810799/1‑140 (MQ=255)                                                                               tACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTga                                                   <  2:1501952/140‑1 (MQ=255)                                                                                acacGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTgag                                                  <  2:104658/140‑1 (MQ=255)                                                                                  acGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTgagag                                                >  1:1494481/1‑140 (MQ=255)                                                                                  acGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTgagag                                                >  1:31241/1‑140 (MQ=255)                                                                                  acGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTgagag                                                <  2:1163482/140‑1 (MQ=255)                                                                                  acGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTgagag                                                <  2:1028980/140‑1 (MQ=255)                                                                                  acGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTGCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTgagag                                                <  2:1452500/140‑1 (MQ=255)                                                                                   cGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTgagaga                                               <  1:324802/140‑1 (MQ=255)                                                                                    gggCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGAt                                              <  1:302285/140‑1 (MQ=255)                                                                                    gggCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGAt                                              <  1:864859/140‑1 (MQ=255)                                                                                       cTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCAc                                           <  1:538558/140‑1 (MQ=255)                                                                                       cTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCAc                                           <  2:673135/140‑1 (MQ=255)                                                                                            cTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTa                                      >  2:895318/1‑140 (MQ=255)                                                                                            cTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTa                                      >  1:423698/1‑140 (MQ=255)                                                                                             tGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTAc                                     <  1:623200/140‑1 (MQ=255)                                                                                              gCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTAcc                                    >  2:289847/1‑140 (MQ=255)                                                                                                cAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGa                                  >  1:667350/1‑140 (MQ=255)                                                                                                cAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGa                                  >  2:37936/1‑140 (MQ=255)                                                                                                  gTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAg                                <  2:636360/140‑1 (MQ=255)                                                                                                     gAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCaa                             <  1:237929/140‑1 (MQ=255)                                                                                                     gAATGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGACAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCaa                             <  1:1392899/140‑1 (MQ=255)                                                                                                      aaTGTGCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGACCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAac                            >  2:958349/1‑140 (MQ=255)                                                                                                        tgtgCCGTTTCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAacac                          <  2:300182/140‑1 (MQ=255)                                                                                                                ttCCGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTg                  >  2:60650/1‑140 (MQ=255)                                                                                                                  ccGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGt                <  1:343843/140‑1 (MQ=255)                                                                                                                   cGCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTc               <  1:453704/140‑1 (MQ=255)                                                                                                                    gCTTTCATCAAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCa              <  1:696936/140‑1 (MQ=255)                                                                                                                         catcaAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGcc         <  1:1240953/140‑1 (MQ=255)                                                                                                                         catcaAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGcc         <  1:709976/140‑1 (MQ=255)                                                                                                                           tcaAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTg       <  1:1324083/140‑1 (MQ=255)                                                                                                                           tcaAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTg       <  1:1280268/140‑1 (MQ=255)                                                                                                                           tcaAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTg       <  2:1323289/140‑1 (MQ=255)                                                                                                                           tcaAGCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTg       <  2:95943/140‑1 (MQ=255)                                                                                                                               gCGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCaaa   <  2:796026/140‑1 (MQ=255)                                                                                                                                cGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCAAAt  <  1:216639/140‑1 (MQ=255)                                                                                                                                cGTTACACTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCAAAt  <  2:376475/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      ATGAGCAATTATGCCCAATGAATCTACCTCATTGGGCATAATTTGGAACTCATCAGATAGCTTTTGCCATCTTCAGATTACACGGGCTCATCTGCCAGTTGAATGTGCCGTTTCCGCTTTCATCAAGCGTTACGCTGGCAATGGCCGAGGTGGTAAACATAGGCGGCGTTTCGCCCGGACATAACTCGGCAACCAGATACCCGACTAACGGTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCAAAT  >  NC_000913/2456275‑2456541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑