Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,528,811	C→T	D456N (GAC→AAC)	ligA ←	DNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,528,811	0	C	T	100.0%	253.8 / NA	73	D456N (GAC→AAC)	ligA	DNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/39);  total (34/39)

TCCAGCGCGTTAACCACGTTTTGTGCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACAT  >  NC_000913/2528675‑2528947                                                                                                                                         |                                                                                                                                         tCCAGCGCGTTAACCACGTTTTGTGCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGcc                                                                                                                                       <  1:1145166/140‑1 (MQ=255) tCCAGCGCGTTAACCACGTTTTGTGCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGcc                                                                                                                                       <  2:564092/140‑1 (MQ=255)     gcgcGTTAACCACGTTTTGTGCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCAtt                                                                                                                                   <  2:1451651/140‑1 (MQ=255)     gcgcGTTAACCACGTTTTGTGCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCAtt                                                                                                                                   <  2:770775/140‑1 (MQ=255)      cgcgTTAACCACGTTTTGTGCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTc                                                                                                                                  >  2:785740/1‑140 (MQ=255)         gTTAACCACGTTTTGTGCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGt                                                                                                                               <  1:1507978/140‑1 (MQ=255)            aaCCACGTTTTGTGCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCaa                                                                                                                            >  1:1063617/1‑140 (MQ=255)                       gtgCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCa                                                                                                                 <  1:676319/140‑1 (MQ=255)                         gCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCAcg                                                                                                               <  1:740142/140‑1 (MQ=255)                            gATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCAcggcg                                                                                                            >  2:140575/1‑140 (MQ=255)                            gATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCAcggcg                                                                                                            <  2:71794/140‑1 (MQ=255)                             aTTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCAcggcgg                                                                                                           <  2:1063617/140‑1 (MQ=255)                             aTTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCAcggcgg                                                                                                           <  1:204793/140‑1 (MQ=255)                                       cATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAgt                                                                                                 >  2:1102315/1‑140 (MQ=255)                                        aTACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAgtg                                                                                                >  1:271218/1‑140 (MQ=255)                                              tCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAg                                                                                          <  1:549402/140‑1 (MQ=255)                                              tCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAg                                                                                          <  1:424689/140‑1 (MQ=255)                                               ccAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGc                                                                                         <  2:395434/140‑1 (MQ=255)                                               ccAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGc                                                                                         <  2:1369201/140‑1 (MQ=255)                                                cAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCg                                                                                        <  1:860141/140‑1 (MQ=255)                                                cAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCg                                                                                        <  2:1492981/140‑1 (MQ=255)                                                  gTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGCCGGGAAACAAAGTGTTTCAGCGAc                                                                                      <  2:1120956/140‑1 (MQ=255)                                                  gTCCGGTCAGTTTGCCTGCGGAGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGAc                                                                                      >  1:769607/1‑140 (MQ=255)                                                   tCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGAct                                                                                     >  1:440211/1‑140 (MQ=255)                                                   tCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGAct                                                                                     >  1:120776/1‑140 (MQ=255)                                                   tCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGAct                                                                                     >  2:930779/1‑140 (MQ=255)                                                      ggTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCtt                                                                                  >  1:233892/1‑140 (MQ=255)                                                        tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTa                                                                                >  1:1424913/1‑140 (MQ=255)                                                        tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTa                                                                                >  2:535675/1‑140 (MQ=255)                                                             ttGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTg                                                                           >  2:1433754/1‑140 (MQ=255)                                                               gCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTgcg                                                                         <  2:538832/140‑1 (MQ=255)                                                                      gTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCa                                                                  >  2:1124252/1‑140 (MQ=255)                                                                         aGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAAt                                                               <  2:1194390/140‑1 (MQ=255)                                                                            ttGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAg                                                            <  2:54150/140‑1 (MQ=255)                                                                            ttGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAg                                                            <  2:899397/140‑1 (MQ=255)                                                                            ttGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAg                                                            <  2:324232/140‑1 (MQ=255)                                                                              gAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGc                                                          <  1:844447/140‑1 (MQ=255)                                                                              gAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGc                                                          <  1:287387/140‑1 (MQ=255)                                                                                   gATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCg                                                     >  1:362159/1‑140 (MQ=255)                                                                                    aTCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCgg                                                    >  1:1231006/1‑140 (MQ=255)                                                                                    aTCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCgg                                                    >  2:861917/1‑140 (MQ=255)                                                                                      cTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTa                                                  <  1:445041/140‑1 (MQ=255)                                                                                         ccGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAg                                               >  1:1334911/1‑140 (MQ=255)                                                                                            gAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCgg                                            >  1:77375/1‑140 (MQ=255)                                                                                              gTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGc                                          <  2:120776/140‑1 (MQ=255)                                                                                               tATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCa                                         >  2:1243912/1‑140 (MQ=255)                                                                                                  ggACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAAcc                                      <  1:1102315/140‑1 (MQ=255)                                                                                                   gACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCg                                     <  2:1183016/140‑1 (MQ=255)                                                                                                   gACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCg                                     >  1:705203/1‑140 (MQ=255)                                                                                                      atatTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCtt                                  <  2:1486568/140‑1 (MQ=255)                                                                                                       tatTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTc                                 >  1:1303609/1‑140 (MQ=255)                                                                                                        atTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCa                                >  2:409752/1‑140 (MQ=255)                                                                                                        atTCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCa                                >  2:317628/1‑140 (MQ=255)                                                                                                         ttCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCTGGCAACCGCTTCAc                               >  2:1093079/1‑140 (MQ=255)                                                                                                         ttCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCAc                               >  2:280976/1‑140 (MQ=255)                                                                                                         ttCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCAc                               >  2:1463789/1‑140 (MQ=255)                                                                                                         ttCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCAc                               >  1:316063/1‑140 (MQ=255)                                                                                                         ttCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCAc                               >  1:336270/1‑140 (MQ=255)                                                                                                         ttCTTTTTCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCAc                               >  1:865899/1‑140 (MQ=255)                                                                                                               ttCAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTccc                         >  1:1083920/1‑139 (MQ=255)                                                                                                                 cAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCcaca                       <  1:694353/140‑1 (MQ=255)                                                                                                                 cAACCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCcaca                       <  2:865899/140‑1 (MQ=255)                                                                                                                  aaCCAGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCacac                      >  1:48875/1‑140 (MQ=255)                                                                                                                      aGCTGATCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTc                  <  1:1133959/140‑1 (MQ=255)                                                                                                                           aTCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGt             <  2:1081425/140‑1 (MQ=255)                                                                                                                            tCGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTc            >  2:55566/1‑140 (MQ=255)                                                                                                                             cGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCa           <  1:930779/140‑1 (MQ=255)                                                                                                                             cGATGATTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCa           <  2:1231006/140‑1 (MQ=255)                                                                                                                               atgatTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCCGCGCACCGCAAATCAGGCCACCGGTACAGCGGCCAACCGCTTCACCTTCCACCCACTCAACGTCAGa         >  2:1309401/1‑140 (MQ=255)                                                                                                                                  atTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAAcc      <  2:1129932/140‑1 (MQ=255)                                                                                                                                  atTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAAcc      <  2:77375/140‑1 (MQ=255)                                                                                                                                  atTTTGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAAcc      <  2:271218/140‑1 (MQ=255)                                                                                                                                      tGTTGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACAt  <  1:140575/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TCCAGCGCGTTAACCACGTTTTGTGCCGATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACAT  >  NC_000913/2528675‑2528947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑