Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,587,747	C→T	G51G (GGC→GGT)	acrD →	multidrug efflux pump RND permease AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,587,747	0	C	T	100.0%	287.8 / NA	85	G51G (GGC→GGT)	acrD	multidrug efflux pump RND permease AcrD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/55);  total (30/55)

TTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGCGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGC  >  NC_000913/2587611‑2587881                                                                                                                                         |                                                                                                                                       ttGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCa                                                                                                                                     <  2:1479700/140‑1 (MQ=255) ttGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCa                                                                                                                                     >  2:79390/1‑140 (MQ=255)    aTCGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCg                                                                                                                                  >  1:63470/1‑140 (MQ=255)      cGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGc                                                                                                                                >  2:1338374/1‑140 (MQ=255)      cGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGc                                                                                                                                >  2:397044/1‑140 (MQ=255)      cGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGc                                                                                                                                >  1:514716/1‑140 (MQ=255)      cGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGc                                                                                                                                >  2:955765/1‑140 (MQ=255)         cccATTTTTGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCa                                                                                                                             <  1:1327108/140‑1 (MQ=255)             tttttGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACg                                                                                                                         <  1:439860/140‑1 (MQ=255)             tttttGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACg                                                                                                                         <  2:1314376/140‑1 (MQ=255)             tttttGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACg                                                                                                                         <  1:272504/140‑1 (MQ=255)              ttttGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGc                                                                                                                        >  2:724964/1‑140 (MQ=255)                   ccTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGTCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGaa                                                                                                                   >  2:125611/1‑140 (MQ=255)                   ccTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGaa                                                                                                                   <  1:1398836/140‑1 (MQ=255)                       ggagCTGGCAATCCTGTTGTGTCTGACCGGTACTCTGGCGATTTTTTCATTAACCGTTGACCAATCCCCAGTTCTCGCGCCACCCAATGAGCGAGTGACCGCTAACTATCCCGGTGCATCGCCCCCGACGCTGGAAAaca                                                                                                               >  2:1030389/4‑140 (MQ=255)                       ggTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCT‑ACTATCCCGGTGCATCGGCCCATACGCTGGAAAacac                                                                                                              >  2:43969/1‑140 (MQ=255)                        gTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAacac                                                                                                              <  1:337965/140‑1 (MQ=255)                         tGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACAcc                                                                                                             <  1:112447/140‑1 (MQ=255)                          gCTGGCAATCCTGTTGTGTCTGACAGGTACCCCGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCg                                                                                                            <  2:41973/140‑1 (MQ=255)                            tGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTg                                                                                                          <  2:375196/140‑1 (MQ=255)                            tGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTg                                                                                                          <  2:875364/140‑1 (MQ=255)                              gCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGAc                                                                                                        <  2:522226/140‑1 (MQ=255)                                   ccTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAgg                                                                                                   >  1:65300/1‑140 (MQ=255)                                       ttgtGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTAt                                                                                               >  1:419441/1‑140 (MQ=255)                                        tgtgTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATc                                                                                              <  2:569149/140‑1 (MQ=255)                                           gtCTGACAGGTTCCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAg                                                                                           <  1:1381981/140‑1 (MQ=255)                                           gtCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAg                                                                                           <  2:262986/140‑1 (MQ=255)                                           gtCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAg                                                                                           >  1:878805/1‑140 (MQ=255)                                           gtCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAg                                                                                           <  1:1335959/140‑1 (MQ=255)                                           gtCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAg                                                                                           <  1:687520/140‑1 (MQ=255)                                           gtCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAg                                                                                           <  2:613231/140‑1 (MQ=255)                                            tCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGc                                                                                          >  2:398282/1‑140 (MQ=255)                                              tGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCaa                                                                                        >  2:923536/1‑140 (MQ=255)                                                    gTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATg                                                                                  >  1:944501/1‑140 (MQ=255)                                                    gTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATg                                                                                  >  1:385435/1‑140 (MQ=255)                                                     ttcccTGGCTATTTTTTCATTGCCCGTTGAACAATACGCCGATCACGCGCCACAGAACGTGCGAGTGACTGCTAACTATCCCGGAGCATCGGCCCAGACGCTGGAAAACACCGTGTCCAAGGTTTCCGGGCAAAATATGa                                                                                 <  2:859814/138‑1 (MQ=255)                                                      aCCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGAc                                                                                <  1:480068/140‑1 (MQ=255)                                                          tGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTACCTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGc                                                                            <  1:441221/140‑1 (MQ=255)                                                          tGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCGAAATATGACCGGc                                                                            >  1:992908/1‑140 (MQ=255)                                                          tGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGc                                                                            <  1:1132249/140‑1 (MQ=255)                                                                ttttttCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGAt                                                                      <  2:443796/140‑1 (MQ=255)                                                                ttttttCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGAt                                                                      <  2:1296461/140‑1 (MQ=255)                                                                 tttttCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATa                                                                     >  1:1187306/1‑140 (MQ=255)                                                                 tttttCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATa                                                                     >  2:532998/1‑140 (MQ=255)                                                                 tttttCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATa                                                                     <  2:489635/140‑1 (MQ=255)                                                                 tttttCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATa                                                                     <  1:253486/140‑1 (MQ=255)                                                                  ttttCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATaa                                                                    >  2:298311/1‑140 (MQ=255)                                                                   tttCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCT‑ACTATCCCGGTGCATCGGCCCATACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAAtc                                                                  <  1:43969/140‑1 (MQ=255)                                                                    ttCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAAtc                                                                  <  1:379561/140‑1 (MQ=255)                                                                    ttCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAAtc                                                                  <  1:375832/140‑1 (MQ=255)                                                                      cATTGCCCGTTGACCAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGCCCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAAtctc                                                                <  1:504693/140‑1 (MQ=255)                                                                      cATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAAtctc                                                                <  1:1182823/140‑1 (MQ=255)                                                                      cATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAAtctc                                                                <  1:1201309/140‑1 (MQ=255)                                                                      cATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAAtctc                                                                <  1:788853/140‑1 (MQ=255)                                                                         tGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATg                                                             <  1:923536/140‑1 (MQ=255)                                                                          gCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGt                                                            >  1:1021051/1‑140 (MQ=255)                                                                           cccGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGta                                                           <  2:1277926/140‑1 (MQ=255)                                                                           cccGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGta                                                           <  1:753415/140‑1 (MQ=255)                                                                                tGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCTGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGt                                                      <  2:514716/140‑1 (MQ=255)                                                                                tGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGt                                                      <  1:951890/140‑1 (MQ=255)                                                                                tGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGt                                                      <  2:969795/140‑1 (MQ=255)                                                                                tGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGt                                                      <  2:996937/140‑1 (MQ=255)                                                                                tGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGAGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGt                                                      <  1:486734/140‑1 (MQ=255)                                                                                     aaTACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCAtct                                                 <  1:955765/140‑1 (MQ=255)                                                                                     aaTACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCAtct                                                 <  1:1338374/140‑1 (MQ=255)                                                                                        aCCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCag                                              <  2:1234983/140‑1 (MQ=255)                                                                                        aCCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCag                                              <  2:726833/140‑1 (MQ=255)                                                                                          cccGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCagag                                            >  1:1190057/1‑140 (MQ=255)                                                                                            cGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCa                                          >  1:1076107/1‑140 (MQ=255)                                                                                            cGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCa                                          <  2:495859/140‑1 (MQ=255)                                                                                             gATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAg                                         <  2:1437562/140‑1 (MQ=255)                                                                                               tctcGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTg                                       >  1:1164435/1‑140 (MQ=255)                                                                                                  cgcgCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCa                                    <  2:578389/140‑1 (MQ=255)                                                                                                  cgcgCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCa                                    >  2:715656/1‑140 (MQ=255)                                                                                                    cgcCACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCAcc                                  <  2:63470/140‑1 (MQ=255)                                                                                                       cACCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGt                               <  2:772031/140‑1 (MQ=255)                                                                                                        aCCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTc                              >  1:1378984/1‑140 (MQ=255)                                                                                                        aCCGAATGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTc                              <  1:567127/140‑1 (MQ=255)                                                                                                            aaTGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGc                          <  2:385435/140‑1 (MQ=255)                                                                                                            aaTGTGCGAGTGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGc                          <  1:216463/140‑1 (MQ=255)                                                                                                                      tGACCGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACt                >  2:235412/1‑140 (MQ=255)                                                                                                                         ccGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTa             <  1:391983/140‑1 (MQ=255)                                                                                                                         ccGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTa             <  2:65300/140‑1 (MQ=255)                                                                                                                          cGCTAACTATCCCGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTaa            >  1:60470/1‑140 (MQ=255)                                                                                                                                    cccGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGc  >  1:955273/1‑140 (MQ=255)                                                                                                                                    cccGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGc  <  2:1076107/140‑1 (MQ=255)                                                                                                                                    cccGGTGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGc  >  1:1339364/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGTTGTGTCTGACAGGTACCCTGGCGATTTTTTCATTGCCCGTTGAACAATACCCCGATCTCGCGCCACCGAATGTGCGAGTGACCGCTAACTATCCCGGCGCATCGGCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGC  >  NC_000913/2587611‑2587881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑