Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,904,086	G→A	intergenic (+712/+661)	ygcE → / ← queE	putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,904,086	0	G	A	100.0%	291.2 / NA	85	intergenic (+712/+661)	ygcE/queE	putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/37);  total (48/37)

ATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGG  >  NC_000913/2903949‑2904219                                                                                                                                          |                                                                                                                                      atatGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACt                                                                                                                                     >  1:461871/1‑140 (MQ=255) atatGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACt                                                                                                                                     >  2:341543/1‑140 (MQ=255)   atGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTgg                                                                                                                                   >  1:860945/1‑140 (MQ=255)   atGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTgg                                                                                                                                   >  1:874125/1‑140 (MQ=255)   atGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTgg                                                                                                                                   >  2:1342256/1‑140 (MQ=255)     gTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGgcg                                                                                                                                 >  2:1472337/1‑140 (MQ=255)      ttGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGgcgc                                                                                                                                >  1:239776/1‑140 (MQ=255)      ttGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGgcgc                                                                                                                                >  2:505215/1‑140 (MQ=255)        gCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGGACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCgg                                                                                                                              >  2:1444500/1‑140 (MQ=255)          ttattaCAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCgggg                                                                                                                            >  2:733662/1‑140 (MQ=255)           tattaCAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGa                                                                                                                           >  2:996098/1‑140 (MQ=255)           tattaCAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGa                                                                                                                           >  1:561092/1‑140 (MQ=255)            attaCAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGaa                                                                                                                          <  1:1254252/140‑1 (MQ=255)             ttaCAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAac                                                                                                                         >  2:943018/1‑140 (MQ=255)                     aCTGCTAATAT‑AAAACTTGAGAAAGAGATAACGGGGTATATGGGGGGTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCCGTAGTCTCGGCAGGCTCCGGGTTATCCCCACTGGCGCGGGGAACACCTGgttt                                                                                                                >  2:212605/1‑140 (MQ=255)                      cTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGtttt                                                                                                                >  2:1167355/1‑140 (MQ=255)                      cTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGtttt                                                                                                                <  2:57043/140‑1 (MQ=255)                      cTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGtttt                                                                                                                >  1:346608/1‑140 (MQ=255)                       tGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTc                                                                                                               >  1:763069/1‑140 (MQ=255)                        gCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCg                                                                                                              >  1:1015640/1‑140 (MQ=255)                        gCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCg                                                                                                              <  1:1472337/140‑1 (MQ=255)                          tAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCa                                                                                                            <  1:1005573/140‑1 (MQ=255)                          tAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCa                                                                                                            >  2:1235349/1‑140 (MQ=255)                            atatAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCaaa                                                                                                          >  1:287454/1‑140 (MQ=255)                             tataAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAAt                                                                                                         >  2:132489/1‑140 (MQ=255)                              ataAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATc                                                                                                        <  1:936207/140‑1 (MQ=255)                              ataAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATc                                                                                                        >  2:86544/1‑140 (MQ=255)                                 aaaaCTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTAt                                                                                                     >  1:659502/1‑140 (MQ=255)                                 aaaaCTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTAt                                                                                                     >  1:1203606/1‑140 (MQ=255)                                  aaaCTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATg                                                                                                    <  2:201832/140‑1 (MQ=255)                                   aaCTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATgg                                                                                                   >  2:531099/1‑140 (MQ=255)                                    aCTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGa                                                                                                  <  2:874125/140‑1 (MQ=255)                                    aCTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGa                                                                                                  <  2:1161349/140‑1 (MQ=255)                                        gagaAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTAt                                                                                              <  1:667015/140‑1 (MQ=255)                                        gagaAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTAt                                                                                              >  1:1109485/1‑140 (MQ=255)                                        gagaAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTAt                                                                                              >  1:252786/1‑140 (MQ=255)                                               agaTAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTAtt                                                                                       <  1:733662/140‑1 (MQ=255)                                                         ttATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATcc                                                                             >  1:253717/1‑140 (MQ=255)                                                            tatGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCg                                                                          <  1:1190935/140‑1 (MQ=255)                                                                gtggtTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTgg                                                                      >  2:1507560/1‑140 (MQ=255)                                                                gtggtTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTgg                                                                      <  2:1204045/140‑1 (MQ=255)                                                                 tggtTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGgc                                                                     >  2:206881/1‑140 (MQ=255)                                                                 tggtTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGgc                                                                     >  2:1060735/1‑140 (MQ=255)                                                                    tttATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGcgcg                                                                  <  2:943128/140‑1 (MQ=255)                                                                      tATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCggg                                                                >  2:632138/1‑140 (MQ=255)                                                                      tATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCggg                                                                >  1:1453301/1‑140 (MQ=255)                                                                             gCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACg                                                         >  1:1117429/1‑140 (MQ=255)                                                                             gCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACg                                                         <  2:714272/140‑1 (MQ=255)                                                                                 gcgcGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGgcg                                                     <  1:505215/140‑1 (MQ=255)                                                                                  cgcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGgcgc                                                    >  2:646540/1‑140 (MQ=255)                                                                                  cgcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGgcgc                                                    >  2:833512/1‑140 (MQ=255)                                                                                   gcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCa                                                   <  1:132489/140‑1 (MQ=255)                                                                                   gcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCa                                                   <  2:346608/140‑1 (MQ=255)                                                                                   gcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCa                                                   <  2:840877/140‑1 (MQ=255)                                                                                   gcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCa                                                   <  2:1469003/140‑1 (MQ=255)                                                                                   gcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCa                                                   <  1:1342256/140‑1 (MQ=255)                                                                                   gcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCa                                                   >  1:1333993/1‑140 (MQ=255)                                                                                   gcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCa                                                   <  1:1259976/140‑1 (MQ=255)                                                                                   gcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCa                                                   <  2:1109485/140‑1 (MQ=255)                                                                                   gcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCa                                                   <  2:566467/140‑1 (MQ=255)                                                                                   gcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCa                                                   <  1:1067344/140‑1 (MQ=255)                                                                                     ggggAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACg                                                 <  2:287454/140‑1 (MQ=255)                                                                                     ggggAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACg                                                 <  2:294575/140‑1 (MQ=255)                                                                                           cTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATAc                                           >  1:233038/1‑140 (MQ=255)                                                                                               aCAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAg                                       <  2:233038/140‑1 (MQ=255)                                                                                                cAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGc                                      <  1:1483917/140‑1 (MQ=255)                                                                                                 aGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCAGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGcc                                     <  2:461871/140‑1 (MQ=255)                                                                                                   aaCGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCgt                                   >  2:46252/1‑140 (MQ=255)                                                                                                     cGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCgtgt                                 <  2:1203606/140‑1 (MQ=255)                                                                                                           cAGTAGTCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGc                           <  1:1385393/140‑1 (MQ=255)                                                                                                            agtagtCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCt                          <  1:1486880/140‑1 (MQ=255)                                                                                                            agtagtCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCt                          <  1:1053882/140‑1 (MQ=255)                                                                                                            agtagtCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCt                          <  1:1060735/140‑1 (MQ=255)                                                                                                              tagtCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCg                        <  1:646540/140‑1 (MQ=255)                                                                                                                gtCTCGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGt                      >  2:822511/1‑140 (MQ=255)                                                                                                                 tctcGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGtt                     >  2:775385/1‑140 (MQ=255)                                                                                                                  ctcGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGttt                    >  1:1130277/1‑140 (MQ=255)                                                                                                                  ctcGTCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGttt                    >  1:743622/1‑140 (MQ=255)                                                                                                                      tCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGTCTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATcc                >  1:465286/1‑140 (MQ=255)                                                                                                                      tCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATcc                >  2:1063365/1‑140 (MQ=255)                                                                                                                      tCAGGCTCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATcc                >  1:1439294/1‑140 (MQ=255)                                                                                                                      tCAGGCTCCGGTTTACCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATcc                >  2:1133532/1‑140 (MQ=255)                                                                                                                            tCCGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTg          <  2:1453301/140‑1 (MQ=255)                                                                                                                             ccGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTgg         >  2:1025083/1‑140 (MQ=255)                                                                                                                             ccGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTgg         >  2:545069/1‑140 (MQ=255)                                                                                                                              cGGTTTATCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGgc        <  2:659502/140‑1 (MQ=255)                                                                                                                                    aTCCCCACTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCgggg  >  2:1499460/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      ATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGG  >  NC_000913/2903949‑2904219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑