Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,045,565	T→C	T113A (ACG→GCG)	ygfF ←	putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,045,565	0	T	C	100.0%	312.0 / NA	82	T113A (ACG→GCG)	ygfF	putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/41);  total (41/41)

TGCCGCGTAATCAACATATTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACA  >  NC_000913/3045428‑3045696                                                                                                                                           |                                                                                                                                    ggCCGCGAAATAACCATATCCCCCGGGCGAACCCAC‑CCGTGGGGCCCCCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCa                                                                                                                                   <  2:1507570/139‑1 (MQ=255)  gCCGCGTAATCAACATATTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCAc                                                                                                                                  >  1:1339708/1‑140 (MQ=255)    cgcgTAATCAACATATTCCCCTGGCGAACCCAC‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGt                                                                                                                                <  1:1281741/140‑1 (MQ=255)    cgcgTAATCAACATATTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGt                                                                                                                                >  1:917248/1‑140 (MQ=255)    cgcgTAATCAACATATTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGt                                                                                                                                >  2:1422300/1‑140 (MQ=255)      cgTAATCAACATATTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTtg                                                                                                                              >  2:394765/1‑140 (MQ=255)      cgTAATCAACATATTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTtg                                                                                                                              >  1:854537/1‑140 (MQ=255)      cgTAATCAACATATTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTtg                                                                                                                              >  1:1245111/1‑140 (MQ=255)          aTCAACATATTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTtggtgg                                                                                                                          >  2:1270987/1‑140 (MQ=255)               cATATTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGt                                                                                                                     >  1:642707/1‑140 (MQ=255)                atatTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTa                                                                                                                    >  2:1318794/1‑140 (MQ=255)                    tCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCg                                                                                                                >  1:349563/1‑140 (MQ=255)                    tCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCg                                                                                                                >  2:1507661/1‑140 (MQ=255)                     ccccTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCgg                                                                                                               >  1:425876/1‑140 (MQ=255)                       ccTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGtt                                                                                                             >  1:977804/1‑140 (MQ=255)                         tGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGa                                                                                                           >  1:448589/1‑140 (MQ=255)                         tGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGa                                                                                                           >  1:1119478/1‑140 (MQ=255)                         tGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGa                                                                                                           >  2:795661/1‑140 (MQ=255)                          ggCGAACCCAC‑CCGTGAGGCCCCCGAGGAGGCATAGCCGTGGGGGCGCCCCCGGCCACCTTTTATAGGCGCCATGGGTTTTGCCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGAt                                                                                                          <  2:934480/140‑1 (MQ=255)                          ggCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGAt                                                                                                          >  2:546671/1‑140 (MQ=255)                          ggCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGAt                                                                                                          <  1:1330678/140‑1 (MQ=255)                              aaCCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGc                                                                                                      >  2:801969/1‑140 (MQ=255)                              aaCCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGc                                                                                                      >  1:860160/1‑140 (MQ=255)                               aCCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCAGCCACGTTGGTGGAAAGTACTCGGTTGATTCGct                                                                                                     <  1:1135404/140‑1 (MQ=255)                                cccAACCCGTGGGCCCCCGGAAGGGACTTTGGCGATACCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGct                                                                                                     <  2:1296004/140‑1 (MQ=255)                                cccAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGctc                                                                                                    >  1:848333/1‑140 (MQ=255)                                cccAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGctc                                                                                                    >  2:1126814/1‑140 (MQ=255)                                         tGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTaa                                                                                            >  2:158859/1‑140 (MQ=255)                                          gAGGCCCCCGAAGAGACTTTGCCGAAGGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTCCCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAg                                                                                           <  1:971646/140‑1 (MQ=255)                                               cACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGtttt                                                                                      <  2:953209/140‑1 (MQ=255)                                               cACCGAAGAGACATGGACGAAAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGtttt                                                                                      <  2:500128/140‑1 (MQ=255)                                                  cGAAGAGACATGGACGATAGCGCCGCCCCTGCCCCCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCaa                                                                                   <  2:448589/140‑1 (MQ=255)                                                       agaCATTGACGATAGCGCCGCCCCTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGC‑‑AGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCa                                                                            <  1:1460174/140‑1 (MQ=255)                                                         ccATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCa                                                                            <  2:50049/139‑1 (MQ=255)                                                         aCATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCa                                                                            <  2:443934/140‑1 (MQ=255)                                                         aCATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCa                                                                            <  1:1126814/140‑1 (MQ=255)                                                         aCATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCa                                                                            <  2:1109694/140‑1 (MQ=255)                                                           aTTGCCGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACt                                                                          <  2:590912/140‑1 (MQ=255)                                                           aTTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACt                                                                          >  1:1200082/1‑140 (MQ=255)                                                             tGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTgg                                                                        <  1:1507661/140‑1 (MQ=255)                                                             tGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTgg                                                                        <  1:1369945/140‑1 (MQ=255)                                                              gACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTggg                                                                       <  1:1500950/140‑1 (MQ=255)                                                              gACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTggg                                                                       >  2:254205/1‑140 (MQ=255)                                                               aCGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGt                                                                      <  2:848333/140‑1 (MQ=255)                                                               aCGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGt                                                                      >  2:413669/1‑140 (MQ=255)                                                                   tAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAAc                                                                  >  1:1136271/1‑140 (MQ=255)                                                                    ggcgcCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACa                                                                 <  1:394765/139‑1 (MQ=255)                                                                    aGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACa                                                                 >  2:95349/1‑140 (MQ=255)                                                                      cgccgcCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAg                                                               >  1:1097635/1‑140 (MQ=255)                                                                      cgccgcCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAg                                                               >  2:845006/1‑140 (MQ=255)                                                                      cgccgcCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAg                                                               >  2:1067591/1‑140 (MQ=255)                                                                          gccCCTGCCACATTTTTTAAGCGCCTTGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATcc                                                           <  2:450199/140‑1 (MQ=255)                                                                             aCTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCgg                                                        <  2:917248/140‑1 (MQ=255)                                                                                  caccaTTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTa                                                   <  2:854537/140‑1 (MQ=255)                                                                                      aTTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGa                                               <  1:219000/140‑1 (MQ=255)                                                                                        ttttAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGAcc                                             <  1:619060/140‑1 (MQ=255)                                                                                          ttAGGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAg                                           <  2:641244/140‑1 (MQ=255)                                                                                           tAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAgc                                          <  1:408070/140‑1 (MQ=255)                                                                                           tAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAgc                                          <  2:1136271/140‑1 (MQ=255)                                                                                            aaGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAgcg                                         <  1:546671/140‑1 (MQ=255)                                                                                               cgcCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCgctg                                      >  1:1119238/1‑140 (MQ=255)                                                                                               cgcCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCgctg                                      >  1:89750/1‑140 (MQ=255)                                                                                               cgcCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCgctg                                      >  2:196370/1‑140 (MQ=255)                                                                                                  cATGTGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAATGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTa                                   >  2:1490164/1‑140 (MQ=255)                                                                                                   aTGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAg                                  <  1:1318794/140‑1 (MQ=255)                                                                                                          ttACCGCCTCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCa                           <  1:314941/140‑1 (MQ=255)                                                                                                                ccTCGCGGCAGCAGAGAAAATAATCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGATTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGAGc                     >  2:1096935/1‑140 (MQ=255)                                                                                                                  tCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTg                   >  2:1444773/1‑140 (MQ=255)                                                                                                                  tCGCGGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTg                   <  1:158859/140‑1 (MQ=255)                                                                                                                   cgcgGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGa                  >  1:843657/1‑140 (MQ=255)                                                                                                                     gggCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATc                <  2:1152239/139‑1 (MQ=255)                                                                                                                     cgGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATc                >  1:496943/1‑140 (MQ=255)                                                                                                                     cgGCAGCAGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATc                >  2:140966/1‑140 (MQ=255)                                                                                                                       gcagcaGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGa              <  1:95349/140‑1 (MQ=255)                                                                                                                       gcagcaGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGa              <  2:1245111/140‑1 (MQ=255)                                                                                                                       gcagcaGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGa              <  2:1112735/140‑1 (MQ=255)                                                                                                                       gcagcaGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGa              <  1:1422300/140‑1 (MQ=255)                                                                                                                       gcagcaGAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGa              <  2:349563/140‑1 (MQ=255)                                                                                                                         agcagAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGAtt            <  1:685990/140‑1 (MQ=255)                                                                                                                           cagAGAAAATATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGc          >  1:843757/1‑140 (MQ=255)                                                                                                                                  aaTATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAAc   >  1:401358/1‑140 (MQ=255)                                                                                                                                  aaTATCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAAc   >  1:986287/1‑140 (MQ=255)                                                                                                                                   atatCCCGCCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACa  <  1:196370/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                    TGCCGCGTAATCAACATATTCCCCTGGCGAACCCAA‑CCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACA  >  NC_000913/3045428‑3045696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑