Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,138,587	A→G	V150A (GTC→GCC)	gldA ←	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,138,587	0	A	G	100.0%	220.6 / NA	59	V150A (GTC→GCC)	gldA	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/35);  total (24/35)

CTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGA  >  NC_000913/4138454‑4138719                                                                                                                                         |                                                                                                                                     cTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCatat                                                                                                                                >  1:414003/1‑140 (MQ=255) cTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCatat                                                                                                                                >  1:205842/1‑140 (MQ=255)  ttGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATAtt                                                                                                                               >  1:537036/1‑140 (MQ=255)     ccgcccgcCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCgg                                                                                                                            >  2:1422917/1‑140 (MQ=255)     ccgcccgcCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCgg                                                                                                                            >  1:84292/1‑140 (MQ=255)       gcccgccATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGt                                                                                                                          >  2:479262/1‑140 (MQ=255)                       gcgcCGCTACGAGAGCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAAcagcag                                                                                                          >  2:137127/1‑140 (MQ=255)                          ccgcTACGAGAGCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATa                                                                                                       >  2:244479/1‑140 (MQ=255)                           cgcTGCGAGGGCGGGCCCGCCCTACAACCCG‑GGTCG‑CCG‑GCGCATCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAg                                                                                                      <  1:17252/140‑1 (MQ=255)                                    ggcgggCACGCGCTCCAACCGG‑GGTCG‑CCG‑GCGCACCCCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAAc                                                                                             <  1:797473/136‑1 (MQ=255)                                     gCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGGCAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACt                                                                                            >  2:710307/1‑140 (MQ=255)                                             gcccattaaaCCC‑GGGTG‑CCGGGCGCACGGCCGTTCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTc                                                                                     <  2:1507242/133‑1 (MQ=255)                                              gcgctCAAACCGGGGTCG‑CCC‑GCGCACCGCGGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCa                                                                                    <  2:223722/136‑1 (MQ=255)                                              gcgcccAAACCG‑GGGTGCCCG‑GCGCCGCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCa                                                                                    <  2:84292/135‑1 (MQ=255)                                              cgcTACAACCCG‑GGTGG‑CCG‑GCGCACCGCGGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCAt                                                                                   <  2:1036479/140‑1 (MQ=255)                                              cgcTACAACCCG‑GGTGG‑CCA‑GCGCATCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCAt                                                                                   <  1:1169406/140‑1 (MQ=255)                                                  acAAACCG‑GGTCG‑CCG‑GCGCCCCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGgt                                                                               <  1:91585/139‑1 (MQ=255)                                                     acccggggTTG‑CCG‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGgtgt                                                                             <  1:239508/134‑1 (MQ=255)                                                     aacccgggTGG‑CCG‑GCGCCCCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGgtgt                                                                             <  1:1157399/134‑1 (MQ=255)                                                            gTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAAc                                                                      >  2:731533/1‑140 (MQ=255)                                                            gTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAAc                                                                      >  1:126617/1‑140 (MQ=255)                                                            gTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAAc                                                                      >  2:1326477/1‑140 (MQ=255)                                                            gTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAAc                                                                      >  1:776795/1‑140 (MQ=255)                                                            gTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAAc                                                                      >  1:72648/1‑140 (MQ=255)                                                            gTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAAc                                                                      >  2:609833/1‑140 (MQ=255)                                                                  cA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACa                                                                 >  2:723582/1‑140 (MQ=255)                                                                   cgccccATCCCCCATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACa                                                                 <  2:1497097/135‑1 (MQ=255)                                                                     gcgcATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAAt                                                               >  2:1375957/1‑140 (MQ=255)                                                                        gaTCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATgcg                                                            <  1:1445618/139‑1 (MQ=255)                                                                             cctaTACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCa                                                       <  1:1469642/137‑1 (MQ=255)                                                                             ccGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCa                                                       <  1:268159/140‑1 (MQ=255)                                                                             ccGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCa                                                       <  1:723582/140‑1 (MQ=255)                                                                             ccGACACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCa                                                       <  2:754510/140‑1 (MQ=255)                                                                              gttgctccgccgCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCAc                                                      <  2:433865/134‑1 (MQ=255)                                                                              gttcccccGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCAc                                                      <  2:736188/135‑1 (MQ=255)                                                                              ggccccccGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCAc                                                      <  2:725704/135‑1 (MQ=255)                                                                              cgacccccGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCAc                                                      <  1:753753/135‑1 (MQ=255)                                                                              cGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATTGGTGTAGATAACAGACAATGCGCTGCAc                                                      <  1:901898/140‑1 (MQ=255)                                                                              cGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCAc                                                      <  1:479262/140‑1 (MQ=255)                                                                               gacccccGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACg                                                     <  2:150836/136‑1 (MQ=255)                                                                                aTACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACgg                                                    <  1:1422917/140‑1 (MQ=255)                                                                                   cccGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGc                                                 <  1:1039232/140‑1 (MQ=255)                                                                                     cgccgcTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCAt                                               <  1:517093/140‑1 (MQ=255)                                                                                     cgccgcTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCAt                                               >  1:1364814/1‑140 (MQ=255)                                                                                                     gCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGt                               >  1:300365/1‑140 (MQ=255)                                                                                                     gCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGt                               <  2:537036/140‑1 (MQ=255)                                                                                                           gcgcCAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGc                         >  2:804109/1‑140 (MQ=255)                                                                                                              ccAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                      <  1:256536/140‑1 (MQ=255)                                                                                                              ccAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                      <  1:812849/140‑1 (MQ=255)                                                                                                              ccAGCGACGATTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                      >  1:690991/1‑140 (MQ=255)                                                                                                              ccAGAGACGTTTTTGGTGTCGAGAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                      <  2:815668/140‑1 (MQ=255)                                                                                                                   gacgaTTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTa                 >  1:1494212/1‑140 (MQ=255)                                                                                                                   gacgaTTTTGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTa                 >  1:129534/1‑140 (MQ=255)                                                                                                                         tttGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGaa           <  1:772065/140‑1 (MQ=255)                                                                                                                         tttGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGaa           <  2:72648/140‑1 (MQ=255)                                                                                                                         tttGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGaa           <  2:1385284/140‑1 (MQ=255)                                                                                                                         tttGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGaa           <  1:137127/140‑1 (MQ=255)                                                                                                                           tGGTGTCGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAaca         <  1:386551/140‑1 (MQ=255)                                                                                                                                 cGACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATg   <  1:776515/140‑1 (MQ=255)                                                                                                                                  gACAATGGCCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGa  >  1:1501928/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCA‑GGTTG‑CCA‑GCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGA  >  NC_000913/4138454‑4138719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑