Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,178,536	G→A	Q30Q (CAG→CAA)	rplK →	50S ribosomal subunit protein L11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,178,536	0	G	A	100.0%	255.9 / NA	74	Q30Q (CAG→CAA)	rplK	50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/28);  total (46/28)

CGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAGCAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTACCAAGACCCCGCC  >  NC_000913/4178401‑4178670                                                                                                                                        |                                                                                                                                       cGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAgg                                                                                                                                    >  2:25719/1‑140 (MQ=255) cGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAgg                                                                                                                                    >  2:756631/1‑140 (MQ=255)  ggggAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAggg                                                                                                                                   >  2:382055/1‑140 (MQ=255)    ggAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCg                                                                                                                                 >  2:1083846/1‑140 (MQ=255)        ccTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTaaa                                                                                                                             >  2:1132881/1‑140 (MQ=255)        ccTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGACCGCCAGTAGGTCCGGCTCTGGGACAACAGGGCGTaaa                                                                                                                             >  2:498861/1‑140 (MQ=255)        ccTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTACAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTaaa                                                                                                                             >  1:626090/1‑140 (MQ=255)           ctcAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAAcat                                                                                                                          >  2:1320399/1‑140 (MQ=255)            tcAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAAcatc                                                                                                                         >  1:397784/1‑140 (MQ=255)                     ttattaCCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTc                                                                                                                >  1:1165742/1‑140 (MQ=255)                     ttattaCCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTc                                                                                                                >  1:1230287/1‑140 (MQ=255)                         taCCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGTGCGTAAACATCATGGAATTCTGCa                                                                                                            <  2:1120229/140‑1 (MQ=255)                         taCCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCa                                                                                                            <  2:1456997/140‑1 (MQ=255)                         taCCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCa                                                                                                            <  2:969187/140‑1 (MQ=255)                            ccAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAg                                                                                                         >  1:369438/1‑140 (MQ=255)                                cTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGtt                                                                                                     >  2:705439/1‑140 (MQ=255)                                cTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGtt                                                                                                     >  1:227283/1‑140 (MQ=255)                                cTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGtt                                                                                                     >  2:438109/1‑140 (MQ=255)                                   gAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCaa                                                                                                  >  2:90875/1‑140 (MQ=255)                                    aGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAAc                                                                                                 >  2:837519/1‑140 (MQ=255)                                       aaTTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCa                                                                                              <  1:1455802/140‑1 (MQ=255)                                         tttATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCaaa                                                                                            >  2:370585/1‑140 (MQ=255)                                          ttATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCaaaa                                                                                           >  1:871898/1‑140 (MQ=255)                                          ttATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCaaaa                                                                                           >  2:1039047/1‑140 (MQ=255)                                                tGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGAt                                                                                     <  2:45495/140‑1 (MQ=255)                                                   cTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTcc                                                                                  >  1:1130134/1‑140 (MQ=255)                                                   cTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTcc                                                                                  >  1:393538/1‑140 (MQ=255)                                                   cTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTcc                                                                                  >  2:89422/1‑140 (MQ=255)                                                          aGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGaaa                                                                           <  2:248965/140‑1 (MQ=255)                                                           gTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGTCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGaaaa                                                                          <  2:659282/140‑1 (MQ=255)                                                            tACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGaaaaa                                                                         >  2:384513/1‑140 (MQ=255)                                                              cAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAgg                                                                       <  1:1388379/140‑1 (MQ=255)                                                                    tATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGcc                                                                 <  2:1162892/140‑1 (MQ=255)                                                                    tATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGcc                                                                 <  1:1132881/140‑1 (MQ=255)                                                                    tATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGcc                                                                 <  1:1431637/140‑1 (MQ=255)                                                                      tGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGa                                                               >  1:1019191/1‑140 (MQ=255)                                                                      tGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGa                                                               >  2:566430/1‑140 (MQ=255)                                                                      tGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGa                                                               >  1:367263/1‑140 (MQ=255)                                                                      tGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGa                                                               >  1:1299006/1‑140 (MQ=255)                                                                         cAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTc                                                            <  1:837519/140‑1 (MQ=255)                                                                         cAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTc                                                            <  1:756631/140‑1 (MQ=255)                                                                         cAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTc                                                            <  1:25719/140‑1 (MQ=255)                                                                           aGCTGCCGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCg                                                          >  1:437632/1‑140 (MQ=255)                                                                           aGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCg                                                          >  2:1159343/1‑140 (MQ=255)                                                                           aGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCg                                                          >  2:441268/1‑140 (MQ=255)                                                                           aGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCg                                                          >  2:1226034/1‑140 (MQ=255)                                                                            gCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCgg                                                         >  1:1041341/1‑140 (MQ=255)                                                                            gCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCgg                                                         >  1:1045159/1‑140 (MQ=255)                                                                                cAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGgtagt                                                     >  1:225642/1‑140 (MQ=255)                                                                                 aGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCAT‑GAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTaa                                                   >  1:596060/1‑140 (MQ=255)                                                                                    tttcAGCTGGTATGGCTAACCGGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATc                                                 <  1:310735/137‑1 (MQ=255)                                                                                    ttGCTGTTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATc                                                 <  2:966168/140‑1 (MQ=255)                                                                                    ttGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCGACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATc                                                 <  2:1410293/140‑1 (MQ=255)                                                                                    ttGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATc                                                 <  2:397784/140‑1 (MQ=255)                                                                                    ttGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATc                                                 <  1:588473/140‑1 (MQ=255)                                                                                                   cTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGAc                                  <  1:314506/140‑1 (MQ=255)                                                                                                   cTAACCCGAGTCCGCCAGTAGGTCCGACTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGAc                                  <  2:738687/140‑1 (MQ=255)                                                                                                     aaCCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCg                                <  1:566430/140‑1 (MQ=255)                                                                                                      aCCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGt                               <  1:1159343/140‑1 (MQ=255)                                                                                                      aCCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGt                               >  2:55969/1‑140 (MQ=255)                                                                                                       cccGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGtt                              >  2:246282/1‑140 (MQ=255)                                                                                                       cccGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGtt                              >  2:702306/1‑140 (MQ=255)                                                                                                       cccGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGtt                              >  1:379934/1‑140 (MQ=255)                                                                                                            gTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTc                         >  2:970008/1‑140 (MQ=255)                                                                                                            gTCCGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTc                         <  2:1019191/140‑1 (MQ=255)                                                                                                               cGCCAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACt                      >  1:559420/1‑140 (MQ=255)                                                                                                                 ccAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACttt                    >  2:955359/1‑140 (MQ=255)                                                                                                                  cAGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTc                   <  2:1492380/140‑1 (MQ=255)                                                                                                                   aGTAGGTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCg                  <  2:1160894/140‑1 (MQ=255)                                                                                                                       ggTCCGGCTCTGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTAc              >  1:203034/1‑140 (MQ=255)                                                                                                                               tctGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTACCAAGAccc      >  2:1221981/1‑140 (MQ=255)                                                                                                                                ctGGGTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTACCAAGAcccc     <  2:225642/140‑1 (MQ=255)                                                                                                                                   ggTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTACCAAGACCccgcc  <  2:437632/140‑1 (MQ=255)                                                                                                                                   ggTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTACCAAGACCccgcc  >  2:1042085/1‑140 (MQ=255)                                                                                                                                   ggTCAACAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTACCAAGACCccgcc  >  2:118730/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAAGCCTATGTCAAGCTGCAGGTTGCAGCTGGTATGGCTAACCCGAGTCCGCCAGTAGGTCCGGCTCTGGGTCAGCAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTACCAAGACCCCGCC  >  NC_000913/4178401‑4178670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑