Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,743	(A)9→8	coding (1048/1425 nt)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,735	0	A	.	100.0%	186.2 / NA	43	coding (1040/1425 nt)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (27/16);  total (27/16)

TGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAG  >  NC_000913/4637606‑4637870                                                                                                                                  |                                                                                                                                        tGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaaatca                                                                                                                               >  2:886704/1‑137 (MQ=255)        aTTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCa                                                                                                                       >  1:540944/1‑140 (MQ=255)           ggTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGt                                                                                                                    >  1:429008/1‑140 (MQ=255)               gAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACg                                                                                                                <  1:263620/140‑1 (MQ=255)                 aaCGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGcc                                                                                                              <  1:454386/140‑1 (MQ=255)                    gTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCAc                                                                                                           >  1:1057206/1‑140 (MQ=255)                    gTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCAc                                                                                                           >  1:192260/1‑140 (MQ=255)                     ttACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCAcc                                                                                                          <  2:303044/140‑1 (MQ=255)                      tactacGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCg                                                                                                         >  2:276109/1‑140 (MQ=255)                           cGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGtt                                                                                                    <  2:749549/140‑1 (MQ=255)                              cAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCCGAAAAAAAAA‑CACTCTGCATGTTACGCCCACCGAGGTTAAc                                                                                                 >  2:520752/1‑140 (MQ=255)                              cAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAAc                                                                                                 >  1:1001763/1‑140 (MQ=255)                                ggCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGTGGTTAACGt                                                                                               >  2:525611/1‑140 (MQ=255)                                ggCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGt                                                                                               <  1:148617/140‑1 (MQ=255)                                      aCTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTtgctgc                                                                                         >  1:824231/1‑140 (MQ=255)                                          gagaATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGaa                                                                                     >  1:541093/1‑140 (MQ=255)                                            gaATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAAcc                                                                                   >  2:447819/1‑140 (MQ=255)                                              aTCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCgg                                                                                 <  2:192260/140‑1 (MQ=255)                                               gcatCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCCTTATTCCCCCGCCTCACCCGACCGCGCCCCGTGCAGGTGGCA‑ACAAAAAAAGAACCCTGCTCGTTACGCCCGCCGAGGTTAACGGTGCTGCTGAACCGGc                                                                                >  2:607847/4‑140 (MQ=255)                                                    aGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAc                                                                           >  1:822470/1‑140 (MQ=255)                                                    aGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAc                                                                           >  2:231795/1‑140 (MQ=255)                                                         gTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAg                                                                      >  1:447828/1‑140 (MQ=255)                                                         gTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAg                                                                      >  2:1005026/1‑140 (MQ=255)                                                         gTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAg                                                                      >  2:190626/1‑140 (MQ=255)                                                                 gACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCt                                                              <  2:1001763/140‑1 (MQ=255)                                                                    tgctgTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTggg                                                           >  2:927534/1‑140 (MQ=255)                                                                       tgTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCAGCGTTACTGGAGCAGGCGCTGGGaaa                                                        >  2:939992/1‑140 (MQ=255)                                                                         ttGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAAtt                                                      <  2:227549/140‑1 (MQ=255)                                                                           gATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTa                                                    <  1:1005026/140‑1 (MQ=255)                                                                           gATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTa                                                    <  2:541093/140‑1 (MQ=255)                                                                            aTGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTAc                                                   >  2:279241/1‑140 (MQ=255)                                                                                ggcggcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGa                                               >  1:756447/1‑140 (MQ=255)                                                                                 gcggcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGAt                                              >  2:828915/1‑140 (MQ=255)                                                                                         attCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCAt                                      <  1:711182/140‑1 (MQ=255)                                                                                         attCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCAt                                      <  1:231795/140‑1 (MQ=255)                                                                                                  cgTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTAc                             >  1:534519/1‑140 (MQ=255)                                                                                                    tCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTAccc                           >  1:613913/1‑140 (MQ=255)                                                                                                    tCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTAccc                           >  2:914677/1‑140 (MQ=255)                                                                                                           aGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGagag                    <  2:61728/140‑1 (MQ=255)                                                                                                           aGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGagag                    <  2:1057206/140‑1 (MQ=255)                                                                                                            gcgcgcACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATc                                     >  1:621440/1‑122 (MQ=255)                                                                                                            gcgcgcACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATc                                     <  2:621440/122‑1 (MQ=255)                                                                                                                   ccGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCa            <  1:939992/140‑1 (MQ=255)                                                                                                                      tGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCa         >  1:962893/1‑140 (MQ=255)                                                                                                                      tGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCa         >  1:333947/1‑140 (MQ=255)                                                                                                                             ggCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAg  >  1:461310/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        TGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAG  >  NC_000913/4637606‑4637870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑