Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,122,640	(T)5→4	intergenic (‑33/+73)	pyrC ← / ← yceB	dihydroorotase/putative lipid‑binding lipoprotein YceB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,122,636	0	T	.	100.0%	156.0 / NA	36	intergenic (‑29/+77)	pyrC/yceB	dihydroorotase/putative lipid‑binding lipoprotein YceB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (25/11);  total (25/11)

TCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGCCTTTCGCCA  >  NC_000913/1122504‑1122768                                                                                                                                     |                                                                                                                                     tCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGttttgccg                                                                                                                               <  1:942472/140‑5 (MQ=255)   gCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGa                                                                                                                            >  1:123999/1‑140 (MQ=255)                    cAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGa                                                                                                           >  1:72569/1‑140 (MQ=255)                       tttttAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAAc                                                                                                        >  2:401824/1‑140 (MQ=255)                       tttttAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAAc                                                                                                        >  2:1013581/1‑140 (MQ=255)                        ttttAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATTCCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGGTAAGCGGAAACg                                                                                                       <  2:746102/140‑1 (MQ=255)                          ttAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGtt                                                                                                     <  1:1013581/140‑1 (MQ=255)                                tGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCtt                                                                                               >  1:643698/1‑140 (MQ=255)                                 gTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCttt                                                                                              >  1:1039784/1‑140 (MQ=255)                                 gTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCttt                                                                                              >  1:974524/1‑140 (MQ=255)                                     ccATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCAc                                                                                          <  2:5000/140‑1 (MQ=255)                                       aTCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGa                                                                                        <  2:72569/140‑1 (MQ=255)                                                                 cTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTc                                                              >  1:536141/1‑140 (MQ=255)                                                                  tGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCg                                                             <  2:536141/140‑1 (MQ=255)                                                                              cTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTa                                                       >  2:943151/1‑134 (MQ=255)                                                                              cTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTa                                                       <  1:943151/134‑1 (MQ=255)                                                                                        tGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATg                                       >  1:586049/1‑140 (MQ=255)                                                                                         gggATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTa                                                >  1:717304/1‑130 (MQ=255)                                                                                         gggATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTa                                                <  2:717304/130‑1 (MQ=255)                                                                                         gggATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGa                                      >  2:1036812/1‑140 (MQ=255)                                                                                         gggATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGa                                      >  2:639961/1‑140 (MQ=255)                                                                                                tGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTc                               >  2:903107/1‑140 (MQ=255)                                                                                                tGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTc                               >  2:372061/1‑140 (MQ=255)                                                                                                tGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTc                               >  2:835479/1‑140 (MQ=255)                                                                                                tGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTc                               >  1:715120/1‑140 (MQ=255)                                                                                                         tAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATg                                       >  1:261369/1‑123 (MQ=255)                                                                                                         tAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATg                                       <  2:261369/123‑1 (MQ=255)                                                                                                         tAATCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGctt                      <  1:372061/140‑1 (MQ=255)                                                                                                          aaTCTCTATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTCCGCCTGGcttc                     <  2:586049/140‑1 (MQ=255)                                                                                                               ctATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGcttcacttc                >  2:147286/1‑140 (MQ=255)                                                                                                               ctATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGcttcacttc                >  1:432456/1‑140 (MQ=255)                                                                                                                tATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGc                             >  2:589042/1‑126 (MQ=255)                                                                                                                tATGCTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGc                             <  1:589042/126‑1 (MQ=255)                                                                                                                    cTCCGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGc           >  2:216613/1‑140 (MQ=255)                                                                                                                       cGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGCCtt        >  1:435413/1‑140 (MQ=255)                                                                                                                       cGGCTGAAGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGCCtt        >  2:272862/1‑140 (MQ=255)                                                                                                                             aaGGGATG‑TTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGCCTTTCGCCa  >  2:425550/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                     TCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGAAAAATAAAGGGCGCGAATGCGCCCTCGTGATTAATCAGTAAATGGAATGACAATTTCGCCTGGCTTCACTTCAATGCCTTTCGCCA  >  NC_000913/1122504‑1122768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑