Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,714,326	(T)9→8	intergenic (+55/‑51)	dtpA → / → gstA	dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,318	0	T	.	100.0%	115.1 / NA	27	intergenic (+47/‑59)	dtpA/gstA	dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/13);  total (14/13)

ATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTA  >  NC_000913/1714191‑1714452                                                                                                                                |                                                                                                                                       aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCa                                                                                                                           >  1:1057341/1‑140 (MQ=255) aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCa                                                                                                                           >  2:134505/1‑140 (MQ=255) aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCa                                                                                                                           >  1:654764/1‑140 (MQ=255)  tgctgcTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAg                                                                                                                          <  1:134505/140‑1 (MQ=255)      gctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAc                                                                                                                      >  2:182734/1‑140 (MQ=255)      gctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAc                                                                                                                      >  2:1052610/1‑140 (MQ=255)                      cTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTa                                                                                                      <  2:899998/140‑1 (MQ=255)                           ccGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                                                                                 <  1:1052610/140‑1 (MQ=255)                              cATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAg                                                                                              >  1:1015459/1‑140 (MQ=255)                                                  cAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGa                                                                          >  2:592147/1‑140 (MQ=255)                                                    gaaaaaGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTTTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGaaa                                                                        <  1:555596/137‑1 (MQ=255)                                                                 aaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGaaa                                                                        >  1:308164/1‑127 (MQ=255)                                                                 aaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGaaa                                                                        <  2:308164/127‑1 (MQ=255)                                                                 aaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAAc                                                           <  1:768745/140‑1 (MQ=255)                                                                   gcagcCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCg                                                         <  2:873219/140‑1 (MQ=255)                                                                     agcCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCggg                                                       <  2:654764/140‑1 (MQ=255)                                                                             cGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTg                                                 <  1:553806/138‑1 (MQ=255)                                                                             cGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTg                                                 >  2:553806/1‑138 (MQ=255)                                                                             cGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGct                                               >  1:255540/1‑140 (MQ=255)                                                                                   aTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCg                                         <  1:592147/140‑1 (MQ=255)                                                                                    ttCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGc                                        <  2:255540/140‑1 (MQ=255)                                                                                                 ttttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCAcc                           <  2:1015459/140‑1 (MQ=255)                                                                                                   ttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCt                         >  1:116376/1‑140 (MQ=255)                                                                                                       aaGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGt                     <  1:459304/140‑1 (MQ=255)                                                                                                           cGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTgaga                 >  2:480131/1‑140 (MQ=255)                                                                                                           cGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTgaga                 >  1:983938/1‑140 (MQ=255)                                                                                                                          aGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTa  >  1:201984/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                       ATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTA  >  NC_000913/1714191‑1714452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑