Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,194,843	(G)6→7	noncoding (23/145 nt)	sibD ←	small RNA SibD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,194,837	1	.	G	100.0%	159.7 / NA	43	noncoding (29/145 nt)	sibD	small RNA SibD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (24/19);  total (24/19)

GGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGA‑GGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGG  >  NC_000913/3194698‑3194971                                                                                                                                             |                                                                                                                                       ggAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCaga                                                                                                                                         <  2:29088/140‑1 (MQ=255)       agcagGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGA‑gggggg                                                                                                                                  <  1:739816/140‑1 (MQ=255)            ggggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGA‑gggggggagaa                                                                                                                             >  1:534294/1‑135 (MQ=255)                cTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAAtcc                                                                                                                          <  2:623814/140‑1 (MQ=255)                     aCGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATcctccct                                                                                                                     <  1:511327/140‑1 (MQ=255)                     aCGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATcctccct                                                                                                                     <  1:883940/140‑1 (MQ=255)                             gCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTg                                                                                                             <  1:38147/140‑1 (MQ=255)                             gCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTg                                                                                                             <  1:458651/140‑1 (MQ=255)                             gCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATAATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTg                                                                                                             >  1:29323/1‑140 (MQ=255)                              ccccTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGt                                                                                                            <  1:196641/140‑1 (MQ=255)                                 cTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTcc                                                                                                         <  2:1010637/140‑1 (MQ=255)                                  tCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCct                                                                                                        <  2:45423/140‑1 (MQ=255)                                             ggggCCTTACAG‑AGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGtt                                                                                            >  2:950815/1‑140 (MQ=255)                                                    tACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaa                                                                                      >  2:952286/1‑140 (MQ=255)                                                            gAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGc                                                                              >  1:174679/1‑140 (MQ=255)                                                              aaGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGt                                                                            >  2:773160/1‑140 (MQ=255)                                                                gggTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCa                                                                          >  1:855764/1‑140 (MQ=255)                                                                           aaGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa                                                               >  2:634483/1‑140 (MQ=255)                                                                            aGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCAt                                                              >  1:351322/1‑140 (MQ=255)                                                                            aGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCAt                                                              >  1:1043993/1‑140 (MQ=255)                                                                            aGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCAt                                                              <  1:60543/140‑1 (MQ=255)                                                                             gCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCAtt                                                             >  2:568305/1‑140 (MQ=255)                                                                             gCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCAtt                                                             >  1:177156/1‑140 (MQ=255)                                                                             gCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCAtt                                                             <  1:568305/140‑1 (MQ=255)                                                                                 gTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGt                                                                   <  1:847358/130‑1 (MQ=255)                                                                                 gTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGt                                                                   >  2:847358/1‑130 (MQ=255)                                                                                  tcatcaTACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAacac                                                        >  1:910153/1‑140 (MQ=255)                                                                                             aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGc                                             >  2:785640/1‑140 (MQ=255)                                                                                             aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGc                                             >  2:471853/1‑140 (MQ=255)                                                                                                gtgtTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCa                                          >  1:980199/1‑140 (MQ=255)                                                                                                  gttgttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCaaa                                        >  1:175087/1‑140 (MQ=255)                                                                                                        aCTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCg                                  >  1:459795/1‑140 (MQ=255)                                                                                                        aCTCGTAAGCTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCATTGTTCCTTTACTATAGGTTGCAAACAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCg                                  >  2:601088/1‑140 (MQ=37)                                                                                                            gTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGa                              <  1:773160/140‑1 (MQ=255)                                                                                                            gTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGa                              <  1:785640/140‑1 (MQ=255)                                                                                                             tAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGTGGAt                             >  1:574637/1‑140 (MQ=255)                                                                                                                gTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGAtttt                          >  2:113963/1‑140 (MQ=255)                                                                                                                   tCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTta                       <  2:29323/140‑1 (MQ=255)                                                                                                                          ttattaACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGc                >  1:731144/1‑140 (MQ=255)                                                                                                                           tattaACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACGCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg               <  1:982158/140‑1 (MQ=255)                                                                                                                           tattaACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg               <  2:351322/140‑1 (MQ=255)                                                                                                                           tattaACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg               <  1:471853/140‑1 (MQ=255)                                                                                                                                   gCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg               >  1:612413/1‑132 (MQ=255)                                                                                                                                   gCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg               <  2:612413/132‑1 (MQ=255)                                                                                                                                       aTCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAg   >  2:3300/1‑140 (MQ=255)                                                                                                                                       aTCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAg   >  1:450456/1‑140 (MQ=255)                                                                                                                                        tCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAgg  <  1:113963/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                       GGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGA‑GGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGG  >  NC_000913/3194698‑3194971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑