Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,966,455	T→C	I660M (ATA→ATG)	ptsP ←	phosphoenolpyruvate‑protein phosphotransferase PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,966,455	0	T	C	100.0%	369.9 / NA	97	I660M (ATA→ATG)	ptsP	phosphoenolpyruvate‑protein phosphotransferase PtsP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/54);  total (43/54)

TGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACA  >  NC_000913/2966319‑2966587                                                                                                                                         |                                                                                                                                     tGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCAtt                                                                                                                                   >  1:878573/1‑140 (MQ=255)     ccgcAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCg                                                                                                                               >  1:77040/1‑140 (MQ=255)      cgcAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGc                                                                                                                              >  2:192046/1‑140 (MQ=255)      cgcAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGc                                                                                                                              >  2:350783/1‑140 (MQ=255)      cgcAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGc                                                                                                                              >  1:630912/1‑140 (MQ=255)      cgcAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGc                                                                                                                              >  1:552511/1‑140 (MQ=255)           cagGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTccc                                                                                                                         >  2:797926/1‑140 (MQ=255)              gTATTTTGCCCGGGCTCCGGAGCGTCCGTTCAAAGACAGACGGCGATTCCCAAGCCCAATGAGGATTGCCCCGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTCCCGCTTCCCggg                                                                                                                      <  2:984143/140‑1 (MQ=255)              gTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCggg                                                                                                                      <  2:410753/140‑1 (MQ=255)              gTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCggg                                                                                                                      <  2:77040/140‑1 (MQ=255)                  tttGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGat                                                                                                                  <  1:961688/140‑1 (MQ=255)                  tttGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGat                                                                                                                  <  1:797926/140‑1 (MQ=255)                     gCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATTAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGatcat                                                                                                               >  1:101464/1‑140 (MQ=255)                        cgcgCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGc                                                                                                            <  2:81936/140‑1 (MQ=255)                         gcgcTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGcc                                                                                                           >  2:716501/1‑140 (MQ=255)                         gcgcTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGcc                                                                                                           >  1:494985/1‑140 (MQ=255)                          cgcTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCa                                                                                                          <  1:192046/140‑1 (MQ=255)                          cgcTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCa                                                                                                          <  2:552511/140‑1 (MQ=255)                           gcTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCag                                                                                                         <  2:878573/140‑1 (MQ=255)                            cTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCaga                                                                                                        <  1:42332/140‑1 (MQ=255)                                    cGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTaa                                                                                                >  2:198263/1‑140 (MQ=255)                                      tCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCTGGCGATCATCGCCAGAGCTCGTAACa                                                                                              <  2:197456/140‑1 (MQ=255)                                      tCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACa                                                                                              <  1:716501/140‑1 (MQ=255)                                        cGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACAtt                                                                                            <  1:31934/140‑1 (MQ=255)                                         gTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTg                                                                                           >  1:451759/1‑140 (MQ=255)                                         gTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTg                                                                                           >  1:889012/1‑140 (MQ=255)                                          ttCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGc                                                                                          <  2:106837/140‑1 (MQ=255)                                          ttCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGc                                                                                          <  2:584788/140‑1 (MQ=255)                                          ttCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGc                                                                                          <  1:808233/140‑1 (MQ=255)                                                 aCAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGa                                                                                   >  2:89915/1‑140 (MQ=255)                                                 aCAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGa                                                                                   >  2:670493/1‑140 (MQ=255)                                                   aGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAg                                                                                 <  2:564361/140‑1 (MQ=255)                                                    gATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGa                                                                                <  1:198263/140‑1 (MQ=255)                                                    gATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGa                                                                                <  2:600337/140‑1 (MQ=255)                                                    gATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGa                                                                                <  1:650222/140‑1 (MQ=255)                                                     aTGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGAc                                                                               >  1:733075/1‑140 (MQ=255)                                                     aTGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGAc                                                                               <  1:380908/140‑1 (MQ=255)                                                     aTGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGAc                                                                               >  2:962048/1‑140 (MQ=255)                                                     aTGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACACTGCAGGATGAAGAc                                                                               >  2:149463/1‑140 (MQ=255)                                                           aTACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCAt                                                                         >  1:172900/1‑140 (MQ=255)                                                            tACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATa                                                                        >  2:429782/1‑140 (MQ=255)                                                             aCCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATaa                                                                       <  2:202603/140‑1 (MQ=255)                                                             aCCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATaa                                                                       <  2:494985/140‑1 (MQ=255)                                                               ccAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAAt                                                                     >  1:6831/1‑140 (MQ=255)                                                                      cAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGcc                                                              >  1:1051986/1‑140 (MQ=255)                                                                        aTGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCAc                                                            <  1:691860/140‑1 (MQ=255)                                                                        aTGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCAc                                                            <  2:1051986/140‑1 (MQ=255)                                                                        aTGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCAc                                                            <  2:147468/140‑1 (MQ=255)                                                                        aTGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCAc                                                            >  1:816422/1‑140 (MQ=255)                                                                         tGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCAcc                                                           >  1:925853/1‑140 (MQ=255)                                                                         tGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCAcc                                                           >  1:849182/1‑140 (MQ=255)                                                                          gAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCAccc                                                          <  1:747822/140‑1 (MQ=255)                                                                          gAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCAccc                                                          <  2:849182/140‑1 (MQ=255)                                                                             gATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCggg                                                       >  2:678550/1‑140 (MQ=255)                                                                              aTTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGt                                                      >  1:190960/1‑140 (MQ=255)                                                                               ttGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTa                                                     <  2:106276/140‑1 (MQ=255)                                                                                  ccACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTAttg                                                  >  1:955974/1‑140 (MQ=255)                                                                                  ccACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTAttg                                                  >  2:265326/1‑140 (MQ=255)                                                                                       cacaTGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCg                                             <  2:955974/140‑1 (MQ=255)                                                                                          aTGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATc                                          <  2:6831/140‑1 (MQ=255)                                                                                          aTGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATc                                          >  1:673925/1‑140 (MQ=255)                                                                                          aTGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATc                                          >  2:195811/1‑140 (MQ=255)                                                                                           tGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCa                                         <  1:884009/140‑1 (MQ=255)                                                                                           tGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCa                                         <  1:583003/140‑1 (MQ=255)                                                                                           tGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGAGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCa                                         <  1:726340/140‑1 (MQ=255)                                                                                            gggATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCaa                                        >  1:472185/1‑140 (MQ=255)                                                                                            gggATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCaa                                        <  2:925853/140‑1 (MQ=255)                                                                                                tCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGc                                    <  1:728937/140‑1 (MQ=255)                                                                                                tCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGc                                    <  2:1010515/140‑1 (MQ=255)                                                                                                 cgcccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGcc                                   <  2:496522/140‑1 (MQ=255)                                                                                                 cgcccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGcc                                   <  1:962048/140‑1 (MQ=255)                                                                                                 cgcccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGcc                                   <  2:673925/140‑1 (MQ=255)                                                                                                 cgcccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGcc                                   <  1:89915/140‑1 (MQ=255)                                                                                                  gcccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGc                                              <  1:372568/128‑1 (MQ=255)                                                                                                  gcccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGc                                              >  2:372568/1‑128 (MQ=255)                                                                                                    ccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCa                                         >  1:967418/1‑131 (MQ=255)                                                                                                    ccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCa                                         <  2:967418/131‑1 (MQ=255)                                                                                                    ccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGa                                <  2:781938/140‑1 (MQ=255)                                                                                                    ccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGa                                >  1:162259/1‑140 (MQ=255)                                                                                                    ccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGa                                >  2:686884/1‑140 (MQ=255)                                                                                                    ccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGa                                <  1:670493/140‑1 (MQ=255)                                                                                                    ccgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGa                                >  2:127479/1‑140 (MQ=255)                                                                                                     cgccATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGaa                               <  2:822026/140‑1 (MQ=255)                                                                                                        cATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGt                            >  2:92479/1‑140 (MQ=255)                                                                                                        cATTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGt                            >  1:812226/1‑140 (MQ=255)                                                                                                         aTTTCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTa                           <  2:172900/140‑1 (MQ=255)                                                                                                           ttCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTAtt                         <  2:812226/140‑1 (MQ=255)                                                                                                            tCACCGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTg                        <  1:465530/140‑1 (MQ=255)                                                                                                               ccGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGt                     <  2:816422/140‑1 (MQ=255)                                                                                                               ccGCACAAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGt                     <  1:195811/140‑1 (MQ=255)                                                                                                                  cacaAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAg                  <  1:874363/140‑1 (MQ=255)                                                                                                                   acaAACGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGa                 >  2:708775/1‑140 (MQ=255)                                                                                                                      aaCGGAGATCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCg              <  2:451759/140‑1 (MQ=255)                                                                                                                             aTCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGc       >  2:943926/1‑140 (MQ=255)                                                                                                                              tCGATTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGcc      <  1:678550/140‑1 (MQ=255)                                                                                                                                 aTTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGcc      >  2:365989/1‑137 (MQ=255)                                                                                                                                 aTTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGcc      <  1:365989/137‑1 (MQ=255)                                                                                                                                 aTTCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAAc   >  1:18209/1‑140 (MQ=255)                                                                                                                                  ttCCATGCATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACa  >  2:158002/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACA  >  NC_000913/2966319‑2966587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑