Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,004,250	T→C	T513A (ACG→GCG)	ygeV ←	putative sigma(54)‑dependent transcriptional regulator YgeV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,004,250	0	T	C	100.0%	299.2 / NA	79	T513A (ACG→GCG)	ygeV	putative sigma(54)‑dependent transcriptional regulator YgeV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/40);  total (39/40)

CTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGAT  >  NC_000913/3004113‑3004378                                                                                                                                          |                                                                                                                                 cTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAgcgcgc                                                                                                                                >  2:559754/1‑140 (MQ=255)    gcgGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGt                                                                                                                             >  1:770521/1‑140 (MQ=255)           aTTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAAc                                                                                                                      <  2:751700/140‑1 (MQ=255)               gCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCa                                                                                                                  <  2:79429/140‑1 (MQ=255)                cTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCg                                                                                                                           >  2:27657/1‑130 (MQ=255)                cTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCg                                                                                                                           <  1:27657/130‑1 (MQ=255)                cTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCAc                                                                                                                 <  2:725206/140‑1 (MQ=255)                   ttCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTg                                                                                                              >  1:637046/1‑140 (MQ=255)                    tCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGa                                                                                                             >  1:151162/1‑140 (MQ=255)                    tCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGa                                                                                                             >  1:909306/1‑140 (MQ=255)                     ccATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGaa                                                                                                            >  1:954692/1‑140 (MQ=255)                         ctcctcCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGaa                                                                                                        <  2:151162/140‑1 (MQ=255)                         ctcctcCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGaa                                                                                                        <  1:477711/140‑1 (MQ=255)                         ctcctcCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGaa                                                                                                        <  2:770521/140‑1 (MQ=255)                           cctccAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAAcc                                                                                                      <  2:831087/140‑1 (MQ=255)                                aGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGtt                                                                                                 <  1:679737/140‑1 (MQ=255)                                 gcgcCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTa                                                                                                <  2:495456/140‑1 (MQ=255)                                 gcgcCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTa                                                                                                <  1:630135/140‑1 (MQ=255)                                 gcgcCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTa                                                                                                <  2:954692/140‑1 (MQ=255)                                 gcgcCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTa                                                                                                <  2:259761/140‑1 (MQ=255)                                  cgcCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTaa                                                                                               <  1:797816/140‑1 (MQ=255)                                     cGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCa                                                                                            <  2:909306/140‑1 (MQ=255)                                      gTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAg                                                                                           >  2:1024352/1‑140 (MQ=255)                                         ccgcccgcATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGata                                                                                        <  2:307877/140‑1 (MQ=255)                                         ccgcccgcATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGata                                                                                        <  2:822719/140‑1 (MQ=255)                                         ccgcccgcATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGata                                                                                        <  2:637046/140‑1 (MQ=255)                                            cccgcATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTc                                                                                     >  2:439358/1‑140 (MQ=255)                                              cgcATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCa                                                                                   <  1:1024135/140‑1 (MQ=255)                                                  tcatcGAGTGACAGGTGCGCCTCACTGACCTCCGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCaa                                                                               <  2:531807/140‑1 (MQ=255)                                                           gACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTaa                                                                      >  2:896138/1‑140 (MQ=255)                                                            aCAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAAc                                                                     <  2:54468/140‑1 (MQ=255)                                                                gTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTcgc                                                                 >  2:45261/1‑140 (MQ=255)                                                                gTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTcgc                                                                 >  2:810236/1‑140 (MQ=255)                                                                  gcgcCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCgcgc                                                               >  1:100716/1‑140 (MQ=255)                                                                   cgcCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCa                                                              >  2:742189/1‑140 (MQ=255)                                                                   cgcCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCa                                                              >  2:753898/1‑140 (MQ=255)                                                                   cgcCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCa                                                              >  1:989240/1‑140 (MQ=255)                                                                          cTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTcc                                                       >  2:31402/1‑140 (MQ=255)                                                                           tGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCg                                                      <  2:806832/140‑1 (MQ=255)                                                                           tGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCg                                                      >  2:160847/1‑140 (MQ=255)                                                                              ccTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGc                                                   <  2:969366/140‑1 (MQ=255)                                                                               ctctGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGcc                                                  >  2:750445/1‑140 (MQ=255)                                                                               ctctGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGcc                                                  <  2:989240/140‑1 (MQ=255)                                                                                tctGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGTGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCa                                                 >  2:608404/1‑140 (MQ=255)                                                                                  tGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAAc                                               >  2:1058000/1‑140 (MQ=255)                                                                                  tGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAAc                                               >  1:47679/1‑140 (MQ=255)                                                                                  tGTTACATCACTTTCCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGGTTCCGGGCCAAc                                               >  2:762661/1‑140 (MQ=255)                                                                                     tACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGAt                                            <  1:45261/140‑1 (MQ=255)                                                                                     tACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGAt                                            <  1:742189/140‑1 (MQ=255)                                                                                       cATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAATTCGCGCAGTTTTCCGGGCAAACGTTgt                                          >  2:709860/1‑139 (MQ=255)                                                                                       cATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGc                                          <  2:100716/140‑1 (MQ=255)                                                                                       cATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGc                                          <  1:896138/140‑1 (MQ=255)                                                                                       cATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGc                                          >  1:782220/1‑140 (MQ=255)                                                                                         tCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAAc                                               >  1:604174/1‑133 (MQ=255)                                                                                         tCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAAc                                               <  2:604174/133‑1 (MQ=255)                                                                                         tCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCtt                                        >  1:720361/1‑140 (MQ=255)                                                                                         tCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCtt                                        >  2:459536/1‑140 (MQ=255)                                                                                            cTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGTGCAGGTTTCCGGGCCAACGATGc                                          <  2:110168/135‑1 (MQ=255)                                                                                            cTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGTGCAGGTTTCCGGGCCAACGATGc                                          >  1:110168/1‑135 (MQ=255)                                                                                            cTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACg                                     >  1:838220/1‑140 (MQ=255)                                                                                            cTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACg                                     >  2:380023/1‑140 (MQ=255)                                                                                              ttGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCa                                   <  1:439358/140‑1 (MQ=255)                                                                                              ttGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCa                                   >  1:828733/1‑140 (MQ=255)                                                                                                  tCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAatat                               >  2:46576/1‑140 (MQ=255)                                                                                                    cGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATAttt                             >  2:947755/1‑140 (MQ=255)                                                                                                      ttGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcg                           >  1:816581/1‑140 (MQ=255)                                                                                                      ttGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcg                           >  1:580048/1‑140 (MQ=255)                                                                                                         tgCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcgacg                        >  1:25041/1‑140 (MQ=255)                                                                                                         tgCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcgacg                        <  1:234479/140‑1 (MQ=255)                                                                                                         tgCCATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcgacg                        <  1:436721/140‑1 (MQ=255)                                                                                                           ccATTATTCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGGCAACGATGCTTACGCAATATTTCgacgac                      >  2:97582/1‑140 (MQ=255)                                                                                                                attCAGCAGATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCgg                 >  1:201122/1‑140 (MQ=255)                                                                                                                   cagcagATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAg              <  1:46576/140‑1 (MQ=255)                                                                                                                        gATTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATg         <  1:559754/140‑1 (MQ=255)                                                                                                                         aTTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGc        <  1:1024352/140‑1 (MQ=255)                                                                                                                         aTTTGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGc        <  1:380023/140‑1 (MQ=255)                                                                                                                           ttGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcgacg                        <  1:516787/122‑1 (MQ=255)                                                                                                                           ttGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcgacg                        >  2:516787/1‑122 (MQ=255)                                                                                                                           ttGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGc             >  1:703870/1‑133 (MQ=255)                                                                                                                           ttGGCGGCAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGc             <  2:703870/133‑1 (MQ=255)                                                                                                                               cggcAATAGCGCGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGAt  <  1:810236/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 CTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGACCTCTGTTACATCACTTTGCTCCGTTGTGCCATTATTCAGCAGATTTGGCGGCAATAGCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGAT  >  NC_000913/3004113‑3004378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑