Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,119,580	C→T	intergenic (‑468/+17)	yghJ ← / ← glcA	putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,119,580	0	C	T	100.0%	181.8 / NA	51	intergenic (‑468/+17)	yghJ/glcA	putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/22);  total (29/22)

GATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCCC  >  NC_000913/3119445‑3119714                                                                                                                                        |                                                                                                                                       gATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATcc                                                                                                                                    >  1:700928/1‑140 (MQ=255)    gTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGc                                                                                                                                 >  1:733816/1‑140 (MQ=255)       ctcACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCttt                                                                                                                              <  1:240240/140‑1 (MQ=255)         cACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTAt                                                                                                                            <  1:915730/140‑1 (MQ=255)           cGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTg                                                                                                                          <  2:1028370/140‑1 (MQ=255)                ttAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTa                                                                                                                     >  1:277511/1‑140 (MQ=255)                      attTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATGAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGAc                                                                                                               >  2:921768/1‑140 (MQ=255)                      attTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGAc                                                                                                               >  2:720839/1‑140 (MQ=255)                        ttAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTa                                                                                                             >  2:494192/1‑140 (MQ=255)                          aGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAAc                                                                                                           >  2:99920/1‑140 (MQ=255)                            cAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACAt                                                                                                         >  1:280137/1‑140 (MQ=255)                             aGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATc                                                                                                        <  2:427719/140‑1 (MQ=255)                                ttATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCg                                                                                                     <  2:912193/140‑1 (MQ=255)                                  aTCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGt                                                                                                   <  1:586630/140‑1 (MQ=255)                                         cGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGTCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAAcaca                                                                                            >  2:1008644/1‑140 (MQ=255)                                          gCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACAt                                                                                           <  1:494192/140‑1 (MQ=255)                                                 attattGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTg                                                                                    >  1:255055/1‑140 (MQ=255)                                                   tattGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTgca                                                                                  >  2:194265/1‑140 (MQ=255)                                                    attGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTgcag                                                                                 <  2:634499/140‑1 (MQ=255)                                                    attGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTgcag                                                                                 <  2:626387/140‑1 (MQ=255)                                                    attGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTgcag                                                                                 <  2:280137/140‑1 (MQ=255)                                                    attGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTgcag                                                                                 <  2:250888/140‑1 (MQ=255)                                                        ctgcATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAgg                                                                             >  1:1002326/1‑140 (MQ=255)                                                          gcATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGt                                                                           >  2:736007/1‑140 (MQ=255)                                                           cATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTg                                                                          >  1:955030/1‑140 (MQ=255)                                                           cATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTg                                                                          >  2:451259/1‑140 (MQ=255)                                                           cATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTg                                                                          >  1:706176/1‑140 (MQ=255)                                                             tGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGAt                                                                        >  1:355565/1‑140 (MQ=255)                                                              ggATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATa                                                                       <  2:700928/140‑1 (MQ=255)                                                                ataataAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACCCATACGCCTGCAGCAGGGTGATAAt                                                                     <  2:843841/140‑1 (MQ=255)                                                                      aaGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGAt                                                               <  1:99920/140‑1 (MQ=255)                                                                       aGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATa                                                              >  1:944777/1‑140 (MQ=255)                                                                         cGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAAc                                                            >  1:371350/1‑140 (MQ=255)                                                                          gagaAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACg                                                           >  2:462019/1‑140 (MQ=255)                                                                                         aTTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGa                                            <  2:566743/140‑1 (MQ=255)                                                                                                      aataatGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACgg                               <  2:277511/140‑1 (MQ=255)                                                                                                      aataatGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACgg                               <  2:733816/140‑1 (MQ=255)                                                                                                      aataatGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACgg                               >  2:398078/1‑140 (MQ=255)                                                                                                      aataatGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACgg                               >  1:428629/1‑140 (MQ=255)                                                                                                      aataatGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACgg                               >  2:224688/1‑140 (MQ=255)                                                                                                           tGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCa                                  >  1:403399/1‑132 (MQ=255)                                                                                                           tGTAAATGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCa                                  <  2:403399/132‑1 (MQ=255)                                                                                                                aTGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGaa                     <  1:736007/140‑1 (MQ=255)                                                                                                                aTGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGaa                     >  2:757721/1‑140 (MQ=255)                                                                                                                aTGAAGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGaa                     >  2:214621/1‑140 (MQ=255)                                                                                                                    aGCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGt                 >  2:406560/1‑140 (MQ=255)                                                                                                                     gCCGGATGATATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGtt                >  1:760022/1‑140 (MQ=255)                                                                                                                            gatATTAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATtc         <  1:224688/140‑1 (MQ=255)                                                                                                                                ttAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAATGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCgg     >  1:103117/1‑140 (MQ=255)                                                                                                                                ttAACGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCgg     <  2:706176/140‑1 (MQ=255)                                                                                                                                   aCGATTATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGccc  <  1:757721/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCCC  >  NC_000913/3119445‑3119714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑