Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,141,198	T→C	Y30C (TAC→TGC)	hybD ←	putative hydrogenase 2 maturation protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,141,198	0	T	C	100.0%	295.5 / NA	78	Y30C (TAC→TGC)	hybD	putative hydrogenase 2 maturation protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/34);  total (44/34)

ATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTAC  >  NC_000913/3141063‑3141333                                                                                                                                        |                                                                                                                                        atcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCg                                                                                                                                     <  1:71274/140‑1 (MQ=255)        ttCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGctct                                                                                                                              >  1:515479/1‑140 (MQ=255)        ttCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGctct                                                                                                                              >  2:500632/1‑140 (MQ=255)        ttCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGctct                                                                                                                              >  1:173107/1‑140 (MQ=255)         tCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTa                                                                                                                             >  1:103859/1‑140 (MQ=255)         tCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTa                                                                                                                             >  2:1037221/1‑140 (MQ=255)               cgcgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGt                                                                                                                                    >  1:802108/1‑127 (MQ=255)               cgcgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGt                                                                                                                                    <  2:802108/127‑1 (MQ=255)                gcgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTc                                                                                                                      >  2:361364/1‑140 (MQ=255)                   ttCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGAc                                                                                                                   <  2:103859/140‑1 (MQ=255)                           cGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGca                                                                                                           <  1:832565/140‑1 (MQ=255)                                    ggCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATgg                                                                                                  <  2:426682/140‑1 (MQ=255)                                    ggCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCCCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATgg                                                                                                  <  1:540828/140‑1 (MQ=255)                                        tCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTc                                                                                              >  2:486328/1‑140 (MQ=255)                                            cAATAATCAAATGGCCGCGGTTGGCCATGTCCCCAGGCAGCTCCATCCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCg                                                                                          <  2:26859/140‑1 (MQ=255)                                            cAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCg                                                                                          <  2:43883/140‑1 (MQ=255)                                            cAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCg                                                                                          <  2:442679/140‑1 (MQ=255)                                             aataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCgg                                                                                         >  2:207655/1‑140 (MQ=255)                                             aataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCgg                                                                                         <  2:258695/140‑1 (MQ=255)                                              ataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGt                                                                                        <  1:486328/140‑1 (MQ=255)                                                 atCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAg                                                                                     >  1:824040/1‑140 (MQ=255)                                                  tCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGc                                                                                    <  2:890959/140‑1 (MQ=255)                                                    aaaTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAg                                                                                     <  1:266688/137‑1 (MQ=255)                                                    aaaTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAg                                                                                     >  2:266688/1‑137 (MQ=255)                                                    aaaTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaa                                                                                  >  2:812942/1‑140 (MQ=255)                                                       tGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAat                                                                               >  1:597464/1‑140 (MQ=255)                                                         aTCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                                             >  2:631400/1‑140 (MQ=255)                                                         aTCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                                             >  1:104703/1‑140 (MQ=255)                                                         aTCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                                             >  1:1051000/1‑140 (MQ=255)                                                         aTCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                                             >  2:181554/1‑140 (MQ=255)                                                         aTCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                                             >  1:235796/1‑140 (MQ=255)                                                         aTCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                                             >  1:257548/1‑140 (MQ=255)                                                         aTCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                                             >  2:552412/1‑140 (MQ=255)                                                         aTCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                                             >  1:363172/1‑140 (MQ=255)                                                           cgcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                                           >  2:408392/1‑140 (MQ=255)                                                           cgcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                                           >  2:92745/1‑140 (MQ=255)                                                           cgcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                                           >  1:157752/1‑140 (MQ=255)                                                           cgcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                                           >  1:26206/1‑140 (MQ=255)                                                            gcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGc                                                                          <  2:104703/140‑1 (MQ=255)                                                              gATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCg                                                                        <  1:317598/140‑1 (MQ=255)                                                              gATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCg                                                                        <  2:173107/140‑1 (MQ=255)                                                              gATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCg                                                                        <  1:227752/140‑1 (MQ=255)                                                              gATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCg                                                                        <  2:572585/140‑1 (MQ=255)                                                                   gCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAcccc                                                                   <  1:500632/140‑1 (MQ=255)                                                                      aTGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTaa                                                                <  2:972073/140‑1 (MQ=255)                                                                      aTGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTaa                                                                <  1:408392/140‑1 (MQ=255)                                                                        gTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGa                                                              >  2:687573/1‑140 (MQ=255)                                                                        gTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGa                                                              <  2:597464/140‑1 (MQ=255)                                                                          cGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACt                                                            <  1:142339/140‑1 (MQ=255)                                                                                  agcTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg                                                    <  2:1051000/140‑1 (MQ=255)                                                                                  agcTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg                                                    <  2:78927/140‑1 (MQ=255)                                                                                   gcTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGc                                                   >  1:73819/1‑140 (MQ=255)                                                                                    cTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCa                                                  >  2:1041923/1‑140 (MQ=255)                                                                                      ccATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAtt                                                <  1:812942/140‑1 (MQ=255)                                                                                          tCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAg                                            <  2:73819/140‑1 (MQ=255)                                                                                          tCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAg                                            <  1:1037221/140‑1 (MQ=255)                                                                                          tCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAg                                            >  2:173920/1‑140 (MQ=255)                                                                                                 gTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc                                     >  2:270798/1‑140 (MQ=255)                                                                                                 gTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc                                     >  1:225825/1‑140 (MQ=255)                                                                                                 gTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc                                     >  2:759122/1‑140 (MQ=255)                                                                                                 gTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc                                     >  1:674550/1‑140 (MQ=255)                                                                                                    ccgccATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACa                                             >  2:429054/1‑129 (MQ=255)                                                                                                    ccgccATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACa                                             <  1:429054/129‑1 (MQ=255)                                                                                                        cATCGAGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGaaa                              >  1:2876/1‑140 (MQ=255)                                                                                                             aGGATCTCAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACt                         <  2:674550/140‑1 (MQ=255)                                                                                                                    cAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGc                  >  1:1031160/1‑140 (MQ=255)                                                                                                                    cAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGc                  >  1:579843/1‑140 (MQ=255)                                                                                                                    cAACATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGc                  >  1:674669/1‑140 (MQ=255)                                                                                                                      aCATAATCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCg                <  1:270798/140‑1 (MQ=255)                                                                                                                            tCCGGGAGAATGCATCGTTGCTCTAACGCTTCGACAATCCCCCCACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAACCTAAAATACGCATTACATAACCTTCACTGAAACCACTTCGCTGCCGTCAgga          >  2:52862/1‑138 (MQ=255)                                                                                                                            tCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCa          >  2:113480/1‑140 (MQ=255)                                                                                                                            tCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCa          >  1:300015/1‑140 (MQ=255)                                                                                                                            tCCGGCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCa          >  1:1054710/1‑140 (MQ=255)                                                                                                                               ggCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATcc       >  1:903253/1‑140 (MQ=255)                                                                                                                               ggCAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATcc       <  2:318766/140‑1 (MQ=255)                                                                                                                                 cAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCAc     >  1:976927/1‑140 (MQ=255)                                                                                                                                 cAGAATGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCAc     >  2:110632/1‑140 (MQ=255)                                                                                                                                    aaTGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTAc  <  2:1031160/140‑1 (MQ=255)                                                                                                                                    aaTGCATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTAc  <  1:110632/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTAC  >  NC_000913/3141063‑3141333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑