Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,538,415	T→C	V344A (GTG→GCG)	yhgF →	putative RNA‑binding protein YhgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,538,415	0	T	C	100.0%	186.8 / NA	51	V344A (GTG→GCG)	yhgF	putative RNA‑binding protein YhgF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

TGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACA  >  NC_000913/3538280‑3538551                                                                                                                                        |                                                                                                                                         tGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTc                                                                                                                                      >  1:199237/1‑140 (MQ=255) tGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTc                                                                                                                                      >  1:540768/1‑140 (MQ=255) tGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTc                                                                                                                                      >  2:87650/1‑140 (MQ=255) tGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTc                                                                                                                                      >  1:245156/1‑140 (MQ=255) tGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTc                                                                                                                                      >  1:467705/1‑140 (MQ=255) tGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTc                                                                                                                                      >  2:606894/1‑140 (MQ=255)     cgAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATg                                                                                                                                  >  1:1043733/1‑140 (MQ=255)      gAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGc                                                                                                                                 <  2:679871/140‑1 (MQ=255)         cGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCAc                                                                                                                              <  1:87650/140‑1 (MQ=255)            gCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGTTGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTgg                                                                                                                           <  1:380661/140‑1 (MQ=255)                  gATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGc                                                                                                                                 <  1:25204/128‑1 (MQ=255)                  gATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGc                                                                                                                                 >  2:25204/1‑128 (MQ=255)                    tGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACtgg                                                                                                                   >  1:981856/1‑140 (MQ=255)                       aGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACtggtgg                                                                                                                <  2:705294/140‑1 (MQ=255)                         cAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGcg                                                                                                              <  2:895546/140‑1 (MQ=255)                           aTCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGcgac                                                                                                            <  2:467705/140‑1 (MQ=255)                           aTCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGcgac                                                                                                            <  2:380886/140‑1 (MQ=255)                             cAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGcgac                                                                                                            >  1:928977/1‑138 (MQ=255)                             cAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAAATGGTGGcggccc                                                                                                          >  2:523441/1‑139 (MQ=255)                             cAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCATGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGcgac                                                                                                            <  2:928977/138‑1 (MQ=255)                              aaCGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGcgaccga                                                                                                         <  1:523441/140‑1 (MQ=255)                              aaCGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGcgaccga                                                                                                         <  1:231214/140‑1 (MQ=255)                                      tGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGaca                                                                                                       >  2:809213/1‑134 (MQ=255)                                      tGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGaca                                                                                                       <  1:809213/134‑1 (MQ=255)                                            tAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGc                                                                                           <  1:606894/140‑1 (MQ=255)                                                   cACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAAATGGTGGCGACCGACACCATTTACCCGCACACCgg                                                                                    >  1:605040/1‑140 (MQ=255)                                                        tCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAgg                                                                               >  1:561314/1‑140 (MQ=255)                                                        tCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCCGTCGCTGCCACCGGCGAACTGGTGGCGACCGACACCATTTACCCGCACACCGGGCAgg                                                                               >  2:329345/1‑140 (MQ=255)                                                          tgctgATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAg                                                                                >  2:315653/1‑137 (MQ=255)                                                          tgctgATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAg                                                                                <  1:315653/137‑1 (MQ=255)                                                                  gcggcCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAagc                                                                     >  1:281493/1‑140 (MQ=255)                                                                      ccccTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCg                                                                 >  1:91179/1‑140 (MQ=255)                                                                         cTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATg                                                              >  1:729240/1‑140 (MQ=255)                                                                           gCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGAc                                                            <  1:494865/140‑1 (MQ=255)                                                                           gCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGAc                                                            <  1:21248/140‑1 (MQ=255)                                                                                aCTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTtg                                                       >  2:354678/1‑140 (MQ=255)                                                                                      tGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTgcgc                                                 >  2:641137/1‑140 (MQ=255)                                                                                              tGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGaa                                         >  2:363170/1‑140 (MQ=255)                                                                                                ggCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGaaaa                                       >  2:913417/1‑140 (MQ=255)                                                                                                       aTCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAAc                                >  2:22831/1‑140 (MQ=255)                                                                                                       aTCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAAc                                <  2:199237/140‑1 (MQ=255)                                                                                                       aTCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAAc                                >  2:1059051/1‑140 (MQ=255)                                                                                                         ccGGGTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGt                              <  2:540768/140‑1 (MQ=255)                                                                                                            ggTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGa                           >  1:526323/1‑140 (MQ=255)                                                                                                            ggTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGa                           >  1:378160/1‑140 (MQ=255)                                                                                                             gTCTGCGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGaa                          <  1:913417/140‑1 (MQ=255)                                                                                                                  cGTACTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGt                     >  1:777067/1‑140 (MQ=255)                                                                                                                      cTGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCg                 <  1:329345/140‑1 (MQ=255)                                                                                                                       tGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCg                 <  1:167527/139‑1 (MQ=255)                                                                                                                       tGGGGTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCg                 >  2:167527/1‑139 (MQ=255)                                                                                                                         gggTAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATc              <  1:354678/140‑1 (MQ=255)                                                                                                                            tAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGt           <  2:290679/140‑1 (MQ=255)                                                                                                                            tAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGt           <  2:1043733/140‑1 (MQ=255)                                                                                                                            tAAAAGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGt           >  1:616207/1‑140 (MQ=255)                                                                                                                              aaaGTGGCGGCGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTaa         <  2:281493/140‑1 (MQ=255)                                                                                                                                   ggcggcGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATc              >  2:555693/1‑130 (MQ=255)                                                                                                                                   ggcggcGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATc              <  1:555693/130‑1 (MQ=255)                                                                                                                                     cggcggTCGATGCCACTGGCAAACTGGGGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCCATGACCGTTGCTGCGCTGTGTGAAAAACATAACGATGAACTGGTAGCGATCGGGAACGGTACa  >  2:126839/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         TGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACA  >  NC_000913/3538280‑3538551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑