Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	263,792	A→G	H72H (CAT→CAC)	yafW ←	antitoxin of the YkfI‑YafW toxin‑antitoxin pair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	263,792	0	A	G	100.0%	340.2 / NA	91	H72H (CAT→CAC)	yafW	antitoxin of the YkfI‑YafW toxin‑antitoxin pair
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (58/33);  total (58/33)

GGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATGCA  >  NC_000913/263656‑263928                                                                                                                                         |                                                                                                                                         ggTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGtgg                                                                                                                                       >  2:933892/1‑140 (MQ=255)       gTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTggggg                                                                                                                                 <  2:70442/140‑1 (MQ=255)         tttCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCt                                                                                                                               <  1:376581/140‑1 (MQ=255)                   gCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCg                                                                                                                     <  1:91262/140‑1 (MQ=255)                        cGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCggtggt                                                                                                                <  1:516913/140‑1 (MQ=255)                          tgtAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCa                                                                                                              >  2:33633/1‑140 (MQ=255)                             aGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCa                                                                                                           <  2:464640/140‑1 (MQ=255)                              ggTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCAt                                                                                                          <  1:656331/140‑1 (MQ=255)                               gTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCg                                                                                                                     >  2:596084/1‑128 (MQ=255)                               gTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCg                                                                                                                     <  1:596084/128‑1 (MQ=255)                               gTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATc                                                                                                         <  2:319891/140‑1 (MQ=255)                                   ttAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGAtt                                                                                                     <  2:1028753/140‑1 (MQ=255)                                   ttAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGAtt                                                                                                     >  1:490692/1‑140 (MQ=255)                                   ttAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGAtt                                                                                                     >  1:971014/1‑140 (MQ=255)                                   ttAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGAtt                                                                                                     >  1:1028753/1‑140 (MQ=255)                                         cTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCt                                                                                               >  1:933434/1‑140 (MQ=255)                                         cTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCt                                                                                               <  2:1049421/140‑1 (MQ=255)                                         cTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCt                                                                                               >  2:686110/1‑140 (MQ=255)                                         cTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCt                                                                                               >  1:372321/1‑140 (MQ=255)                                         cTGAGTGGGGCAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCt                                                                                               <  2:653114/140‑1 (MQ=255)                                           gAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTgg                                                                                             <  1:109458/140‑1 (MQ=255)                                            aGTGGGGCAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGCACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTgga                                                                                            >  1:439727/1‑140 (MQ=255)                                                   tAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATaaa                                                                                     >  1:713541/1‑140 (MQ=255)                                                      aTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTg                                                                                              <  1:297175/128‑1 (MQ=255)                                                      aTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTg                                                                                  >  2:715033/1‑140 (MQ=255)                                                      aTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTg                                                                                  >  1:382594/1‑140 (MQ=255)                                                      aTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTg                                                                                  >  2:661571/1‑140 (MQ=255)                                                      aTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTg                                                                                  >  1:159067/1‑140 (MQ=255)                                                      aTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTg                                                                                  >  2:292523/1‑140 (MQ=255)                                                      aTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTg                                                                                  >  2:162730/1‑140 (MQ=255)                                                      aTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGGACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTg                                                                                              >  2:297175/1‑128 (MQ=255)                                                           gATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGa                                                                             <  2:490692/140‑1 (MQ=255)                                                           gATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGa                                                                             >  2:758183/1‑140 (MQ=255)                                                             tGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAAt                                                                           >  1:646503/1‑140 (MQ=255)                                                               tataCGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGt                                                                         >  1:391586/1‑140 (MQ=255)                                                               tataCGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGt                                                                         >  2:74786/1‑140 (MQ=255)                                                                ataCGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGtt                                                                        <  1:661571/140‑1 (MQ=255)                                                                 taCGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGttt                                                                       >  2:48798/1‑140 (MQ=255)                                                                 taCGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGttt                                                                       >  1:923429/1‑140 (MQ=255)                                                                       gCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCa                                                                 >  2:994395/1‑140 (MQ=255)                                                                        ccGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAg                                                                >  2:679432/1‑140 (MQ=255)                                                                        ccGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAg                                                                >  2:876118/1‑140 (MQ=255)                                                                        ccGCAACTGCCAAGGGTGTCGGCTTCGCAGATTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAg                                                                >  1:902368/1‑140 (MQ=255)                                                                         cGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGa                                                                             <  1:826821/126‑1 (MQ=255)                                                                         cGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGa                                                                             >  2:826821/1‑126 (MQ=255)                                                                         cGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGc                                                               >  2:887120/1‑140 (MQ=255)                                                                         cGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGc                                                               >  1:147421/1‑140 (MQ=255)                                                                         cGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGc                                                               >  1:674399/1‑140 (MQ=255)                                                                           cAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCtt                                                             >  1:307938/1‑140 (MQ=255)                                                                            aaCTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTc                                                            >  1:288560/1‑140 (MQ=255)                                                                              cTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCg                                                          <  2:258043/140‑1 (MQ=255)                                                                               tGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCgg                                                         <  2:600087/140‑1 (MQ=255)                                                                                 ccAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCa                                                       >  2:737019/1‑140 (MQ=255)                                                                                      ggTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGa                                                  <  2:713541/140‑1 (MQ=255)                                                                                        tgtCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATg                                                >  2:489214/1‑140 (MQ=255)                                                                                          tCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTc                                              <  1:706487/140‑1 (MQ=255)                                                                                           cGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTTGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCa                                             >  1:325700/1‑140 (MQ=255)                                                                                              cTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTg                                          <  2:382594/140‑1 (MQ=255)                                                                                               ttCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGa                                         <  1:795784/140‑1 (MQ=255)                                                                                                tCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTGATCCAGCTTCCGGCATTCGATGTCACTGaa                                        >  2:74526/1‑140 (MQ=255)                                                                                                  gCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACt                                      >  2:760639/1‑140 (MQ=255)                                                                                                   cAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACtt                                     <  1:162730/140‑1 (MQ=255)                                                                                                        tAAATCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCgg                                <  2:646503/140‑1 (MQ=255)                                                                                                           aTCGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGa                             >  1:589014/1‑140 (MQ=255)                                                                                                             cGTTGTGGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATg                           <  2:923429/140‑1 (MQ=255)                                                                                                                tgtgGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTg                        >  1:518210/1‑140 (MQ=255)                                                                                                                tgtgGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTg                        >  1:474137/1‑140 (MQ=255)                                                                                                                tgtgGTACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTg                        >  1:575535/1‑140 (MQ=255)                                                                                                                     tACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCg                   <  2:674399/140‑1 (MQ=255)                                                                                                                     tACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCg                   <  2:364061/140‑1 (MQ=255)                                                                                                                     tACAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCg                   <  1:758183/140‑1 (MQ=255)                                                                                                                       cAGGGTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGt                 >  2:447252/1‑140 (MQ=255)                                                                                                                       cAGGATAACGCTGTGGGAGTGGTGGGAGCTGAGTTCACGGGTGGTCAGCATCGAATCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGATCTTGCCGGTGATGCTGGCCCGGt                 <  2:977843/140‑1 (MQ=255)                                                                                                                         gggTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCa               >  2:856218/1‑140 (MQ=255)                                                                                                                          ggTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAg              <  2:159067/140‑1 (MQ=255)                                                                                                                          ggTAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAg              >  2:372580/1‑140 (MQ=255)                                                                                                                            tAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGcc            >  2:54840/1‑140 (MQ=255)                                                                                                                            tAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGcc            >  1:624192/1‑140 (MQ=255)                                                                                                                            tAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGcc            <  2:372321/140‑1 (MQ=255)                                                                                                                            tAACGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCACCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGcc            >  1:887337/1‑140 (MQ=255)                                                                                                                               cGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGa         >  2:231619/1‑140 (MQ=255)                                                                                                                               cGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGa         >  1:970617/1‑140 (MQ=255)                                                                                                                               cGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGa         <  1:108317/140‑1 (MQ=255)                                                                                                                               cGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGa         >  1:530947/1‑140 (MQ=255)                                                                                                                               cGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGa         >  2:377388/1‑140 (MQ=255)                                                                                                                               cGCAGTGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGa         >  2:918342/1‑140 (MQ=255)                                                                                                                                    tGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATg    >  2:995063/1‑140 (MQ=255)                                                                                                                                    tGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATg    >  2:809175/1‑140 (MQ=255)                                                                                                                                    tGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATg    >  2:187800/1‑140 (MQ=255)                                                                                                                                    tGGGCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATg    >  1:837106/1‑140 (MQ=255)                                                                                                                                     gggCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATGc   <  1:1014136/140‑1 (MQ=255)                                                                                                                                      ggCGTGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATGCa  <  2:307938/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATGCA  >  NC_000913/263656‑263928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑