Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,851,355	A→G	V477A (GTG→GCG)	ilvB ←	acetohydroxy acid synthase I subunit IlvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,851,355	0	A	G	100.0%	153.1 / NA	42	V477A (GTG→GCG)	ilvB	acetohydroxy acid synthase I subunit IlvB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/22);  total (20/22)

TGCGGATCGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGC  >  NC_000913/3851223‑3851491                                                                                                                                     |                                                                                                                                         tGCGGATCGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGccc                                                                                                                                   <  2:539175/140‑1 (MQ=255)   cGGATCGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCa                                                                                                                                 <  2:617564/140‑1 (MQ=255)      aTCGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGTTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAgcg                                                                                                                              <  1:695395/140‑1 (MQ=255)        cGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCt                                                                                                                            >  2:1033690/1‑140 (MQ=255)         ggCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCtt                                                                                                                           >  2:97809/1‑140 (MQ=255)         ggCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCtt                                                                                                                           <  2:308500/140‑1 (MQ=255)         ggCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCtt                                                                                                                           <  1:521304/140‑1 (MQ=255)              cGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCgttg                                                                                                                      <  2:130397/140‑1 (MQ=255)              cGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCgttg                                                                                                                      <  1:437923/140‑1 (MQ=255)                          cacaGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATg                                                                                                          >  2:875624/1‑140 (MQ=255)                             aGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGAtt                                                                                                       >  2:528583/1‑140 (MQ=255)                                  tCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGAc                                                                                                  >  1:502585/1‑140 (MQ=255)                                  tCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGAc                                                                                                  >  1:515263/1‑140 (MQ=255)                                  tCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGAc                                                                                                  >  1:17628/1‑140 (MQ=255)                                       gCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCa                                                                                             >  1:944369/1‑140 (MQ=255)                                        ccGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAg                                                                                            <  2:260966/140‑1 (MQ=255)                                         cGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGc                                                                                           >  1:433099/1‑140 (MQ=255)                                         cGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGc                                                                                           >  2:1045512/1‑140 (MQ=255)                                           aaTCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTg                                                                                         >  1:149353/1‑140 (MQ=255)                                           aaTCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTg                                                                                         >  1:708290/1‑140 (MQ=255)                                            aTCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGa                                                                                        <  1:191701/140‑1 (MQ=255)                                                       aTCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGc                                                                             >  1:198990/1‑140 (MQ=255)                                                         cTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCgg                                                                           <  1:97809/140‑1 (MQ=255)                                                         cTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCgg                                                                           <  2:708290/140‑1 (MQ=255)                                                              tAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGcc                                                                      <  1:1045512/140‑1 (MQ=255)                                                              tAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGcc                                                                      <  2:433099/140‑1 (MQ=255)                                                              tAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGcc                                                                      <  2:184460/140‑1 (MQ=255)                                                               aaaGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCa                                                                     >  1:184892/1‑140 (MQ=255)                                                                aaGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCAt                                                                    <  2:184892/140‑1 (MQ=255)                                                                aaGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCAt                                                                    <  2:449921/140‑1 (MQ=255)                                                                            cccGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATAtt                                                        >  2:953774/1‑140 (MQ=255)                                                                                          ggcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGc                                          >  1:446779/1‑140 (MQ=255)                                                                                          ggcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGc                                          <  2:515263/140‑1 (MQ=255)                                                                                           gcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGcc                                         <  2:808860/140‑1 (MQ=255)                                                                                           gcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGcc                                         >  2:811855/1‑140 (MQ=255)                                                                                                   gCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCgg                                 <  1:953774/140‑1 (MQ=255)                                                                                                     cTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGgag                               <  1:811855/140‑1 (MQ=255)                                                                                                                   aGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCCGAATATTTATCATCAGGCTGCCGTCGCCGGAGAAACACAAAACttt                 >  2:580641/1‑140 (MQ=255)                                                                                                                      ctctGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAAAACAACACTTTTCg              >  2:1027355/1‑140 (MQ=255)                                                                                                                          gTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCCTCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATcc          >  2:309507/1‑140 (MQ=255)                                                                                                                             gCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCggg       <  2:446779/140‑1 (MQ=255)                                                                                                                                  tGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGc  <  2:198990/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TGCGGATCGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGC  >  NC_000913/3851223‑3851491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑