Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,462,323	A→G	L113S (TTA→TCA)	nrdD ←	anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,462,323	0	A	G	100.0%	313.0 / NA	84	L113S (TTA→TCA)	nrdD	anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/45);  total (39/45)

ATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACAT  >  NC_000913/4462187‑4462453                                                                                                                                         |                                                                                                                                   atCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACg                                                                                                                                 <  2:337333/140‑1 (MQ=255) atCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACg                                                                                                                                 <  2:430471/140‑1 (MQ=255) atCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACg                                                                                                                                 <  1:834488/140‑1 (MQ=255)    gCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAgg                                                                                                                              <  2:402109/140‑1 (MQ=255)    gCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAgg                                                                                                                              >  2:532598/1‑140 (MQ=255)      cACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACg                                                                                                                                 <  1:378859/135‑1 (MQ=255)      cACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACg                                                                                                                                 >  2:378859/1‑135 (MQ=255)          cTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTgg                                                                                                                        <  1:273333/140‑1 (MQ=255)             aTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCt                                                                                                                     >  1:996471/1‑140 (MQ=255)              tgtgCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTg                                                                                                                    >  2:48827/1‑140 (MQ=255)              tgtgCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTg                                                                                                                    <  1:645333/140‑1 (MQ=255)               gtgCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCTCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGc                                                                                                                   <  2:724369/140‑1 (MQ=255)               gtgCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGc                                                                                                                   <  1:229817/140‑1 (MQ=255)                  ccTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCg                                                                                                                >  2:268916/1‑140 (MQ=255)                     gCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCgacc                                                                                                             >  2:919083/1‑140 (MQ=255)                      caccacGTCGCGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCgacca                                                                                                            <  1:803097/140‑1 (MQ=255)                         cacGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCgaccagac                                                                                                         <  2:167966/140‑1 (MQ=255)                          acGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCgaccagacc                                                                                                        >  2:957324/1‑140 (MQ=255)                           cGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCgaccagacca                                                                                                       >  2:39100/1‑140 (MQ=255)                                   ggCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGAtctc                                                                                               <  1:547868/140‑1 (MQ=255)                                    gcagcaGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAAGCGTTGGTCTGCTCGACCAGACCACGGATCTcc                                                                                              <  2:144864/140‑1 (MQ=255)                                             gCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAgg                                                                                     >  2:1020666/1‑140 (MQ=255)                                                 aCGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGAc                                                                                 >  1:766234/1‑140 (MQ=255)                                                      cATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGt                                                                            >  2:493816/1‑140 (MQ=255)                                                      cATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGt                                                                            >  2:530554/1‑140 (MQ=255)                                                      cATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGt                                                                            >  1:777418/1‑140 (MQ=255)                                                      cATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGt                                                                            >  2:96531/1‑140 (MQ=255)                                                           tgtTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTc                                                                       >  1:56449/1‑140 (MQ=255)                                                           tgtTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTc                                                                       <  1:211481/140‑1 (MQ=255)                                                           tgtTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTc                                                                       <  1:406601/140‑1 (MQ=255)                                                           tgtTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTc                                                                       <  2:777987/140‑1 (MQ=255)                                                             tttAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCAc                                                                     >  2:790382/1‑140 (MQ=255)                                                              ttAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACg                                                                    >  1:348776/1‑140 (MQ=255)                                                                aGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGtt                                                                  >  1:135436/1‑140 (MQ=255)                                                                aGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGtt                                                                  >  1:1045741/1‑140 (MQ=255)                                                                    aCGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAAt                                                              >  1:367801/1‑140 (MQ=255)                                                                     cGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATg                                                             >  2:495329/1‑140 (MQ=255)                                                                       aTCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTc                                                           <  1:532598/140‑1 (MQ=255)                                                                       aTCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTc                                                           >  1:228062/1‑140 (MQ=255)                                                                       aTCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTc                                                           >  2:839809/1‑140 (MQ=255)                                                                         cccGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTcgc                                                         <  2:1045741/140‑1 (MQ=255)                                                                         cccGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTcgc                                                         <  1:957324/140‑1 (MQ=255)                                                                             gCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATc                                                     <  1:1020666/140‑1 (MQ=255)                                                                              ccAGCAGGTCGCGCTGGGTTGGAATCACCTTGTTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCg                                                    >  1:194696/1‑140 (MQ=255)                                                                                agcagGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTg                                                  <  2:1020034/140‑1 (MQ=255)                                                                                 gcagGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGa                                                 <  2:626728/140‑1 (MQ=255)                                                                                  cagGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTcgc                                                         <  2:743492/131‑1 (MQ=255)                                                                                  cagGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTcgc                                                         >  1:743492/1‑131 (MQ=255)                                                                                  cagGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGa                                                 <  1:462925/139‑1 (MQ=255)                                                                                  cagGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGa                                                 >  2:462925/1‑139 (MQ=255)                                                                                  cagGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGAc                                                >  2:514272/1‑140 (MQ=255)                                                                                  cagGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGAc                                                >  2:622435/1‑140 (MQ=255)                                                                                  cagGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGAc                                                >  1:232280/1‑140 (MQ=255)                                                                                      tCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTa                                            <  2:348776/140‑1 (MQ=255)                                                                                      tCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTa                                            <  2:228062/140‑1 (MQ=255)                                                                                       cgcgCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTAc                                           >  1:183286/1‑140 (MQ=255)                                                                                        gcgcTGGGTTGGAATCACCTTGTTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACt                                          <  2:194696/140‑1 (MQ=255)                                                                                        gcgcTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACt                                          <  2:135436/140‑1 (MQ=255)                                                                                        gcgcTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACt                                          <  2:777418/140‑1 (MQ=255)                                                                                        gcgcTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACt                                          <  1:48827/140‑1 (MQ=255)                                                                                        gcgcTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACt                                          <  2:428104/140‑1 (MQ=255)                                                                                        gcgcTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACt                                          <  1:268916/140‑1 (MQ=255)                                                                                        gcgcTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACt                                          <  2:367801/140‑1 (MQ=255)                                                                                         cgcTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTc                                         <  1:919083/140‑1 (MQ=255)                                                                                            tGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGAt                                      <  1:790382/140‑1 (MQ=255)                                                                                             gggTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATg                                     <  1:493816/140‑1 (MQ=255)                                                                                               gTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACt                                          <  2:1008955/133‑1 (MQ=255)                                                                                               gTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACt                                          >  1:1008955/1‑133 (MQ=255)                                                                                                        aCCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCg                                        <  2:806862/126‑1 (MQ=255)                                                                                                        aCCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCg                                        >  1:806862/1‑126 (MQ=255)                                                                                                        aCCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGc                          >  1:328282/1‑140 (MQ=255)                                                                                                        aCCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGc                          >  1:949895/1‑140 (MQ=255)                                                                                                          cTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCa                        <  1:495329/140‑1 (MQ=255)                                                                                                              cTGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAgttg                    <  2:914987/140‑1 (MQ=255)                                                                                                               tGTCTTTGTTGGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAgttgt                   <  2:766234/140‑1 (MQ=255)                                                                                                                     tgttgGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTAt             <  2:328282/140‑1 (MQ=255)                                                                                                                        tgGCGTTTTCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGa          >  1:13524/1‑140 (MQ=255)                                                                                                                             ttttCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATg            <  2:1026406/133‑1 (MQ=255)                                                                                                                             ttttCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATg            >  1:1026406/1‑133 (MQ=255)                                                                                                                               ttCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTa              <  2:322489/129‑1 (MQ=255)                                                                                                                               ttCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTa              >  1:322489/1‑129 (MQ=255)                                                                                                                               ttCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACa   <  1:530554/140‑1 (MQ=255)                                                                                                                               ttCGTTGAGTGACGACGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACa   >  2:508878/1‑140 (MQ=255)                                                                                                                                tCGTTGAGTGACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACAt  >  2:290715/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   ATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACAT  >  NC_000913/4462187‑4462453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑