Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,596,427	A→G	E146E (GAA→GAG)	lgoD →	L‑galactonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,596,427	0	A	G	100.0%	169.4 / NA	46	E146E (GAA→GAG)	lgoD	L‑galactonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/18);  total (28/18)

TGCTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATGGCGCACAGGTGGTGGTG  >  NC_000913/4596302‑4596562                                                                                                                              |                                                                                                                                        tGCTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAg                                                                                                                           >  1:827454/1‑140 (MQ=255) tGCTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAg                                                                                                                           >  1:827415/1‑140 (MQ=255)   cTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAgcg                                                                                                                         <  2:313857/140‑1 (MQ=255)   cTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGGGAGTATTTGAGCGTGCCGGGGGGGAACATTTTTCCCCCAGACGGGATTGACCCCCAGGCGGCAGCCTTAATTGAACCTTTCGCTATTAacg                                                                                                                         >  2:268818/1‑140 (MQ=255)                     cATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTcgcg                                                                                                       <  2:696629/140‑1 (MQ=255)                      aTTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCgcgc                                                                                                      <  1:268818/140‑1 (MQ=255)                                        ggCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGa                                                                                    >  1:452180/1‑140 (MQ=255)                                         gCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAg                                                                                   <  2:597860/140‑1 (MQ=255)                                             ttttAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAgg                                                                               <  2:189864/140‑1 (MQ=255)                                                tAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGc                                                                            <  2:918740/140‑1 (MQ=255)                                                 aGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCt                                                                           <  1:706078/140‑1 (MQ=255)                                                  gTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCtg                                                                          <  1:181352/140‑1 (MQ=255)                                                       tATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTggtggt                                                                     >  1:567314/1‑140 (MQ=255)                                                       tATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTggtggt                                                                     >  1:860084/1‑140 (MQ=255)                                                          ttGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCa                                                                                 >  1:649873/1‑125 (MQ=255)                                                          ttGAGCGTGCCGGTGCCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCa                                                                                 <  2:649873/125‑1 (MQ=255)                                                            gAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcgggg                                                                <  1:291750/140‑1 (MQ=255)                                                                 tGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcggggcgggg                                                           >  2:124090/1‑140 (MQ=255)                                                                 tGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcggggcgggg                                                           >  1:648261/1‑140 (MQ=255)                                                                 tGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcagggcgggg                                                           >  2:766406/1‑140 (MQ=255)                                                                 tGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGCGGTcggggcgggg                                                           >  2:573539/1‑140 (MQ=255)                                                                   ccGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGcc                                                         >  1:930840/1‑140 (MQ=255)                                                                    cGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCa                                                        >  1:933763/1‑140 (MQ=255)                                                                       tGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcggggc                                                               >  1:382620/1‑130 (MQ=255)                                                                       tGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcggggc                                                               <  2:382620/130‑1 (MQ=255)                                                                          cGAACATTTTGCCCGCAGACGGTATTGACCCGCATGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGt                                                  <  1:278380/140‑1 (MQ=255)                                                                                 tttGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGgcg                                           >  2:103155/1‑140 (MQ=255)                                                                                         cAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCa                                   <  1:660175/140‑1 (MQ=255)                                                                                                      cccGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGGCTGGGCGCGGCGGCAATCGCTAAAGCCg                      >  1:673434/1‑140 (MQ=255)                                                                                                        cGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAAACGCTAAAGCCGAt                    >  1:833122/1‑140 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCaa                                  <  2:276245/124‑1 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCaa                                  >  1:276245/1‑124 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:184749/1‑140 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:375589/1‑140 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  1:495081/1‑140 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:698568/1‑140 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  1:245417/1‑140 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:601798/1‑140 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  1:293300/1‑140 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:652179/1‑140 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:695897/1‑140 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATg                   <  2:9796/139‑1 (MQ=255)                                                                                                          cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATg                   >  1:9796/1‑139 (MQ=255)                                                                                                             gcggcAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCAGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATGgcgc               <  1:766406/140‑1 (MQ=255)                                                                                                              cggcAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATGGCGca              <  1:901842/140‑1 (MQ=255)                                                                                                                  agcaTTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATGGCGCACAgg          <  1:184749/140‑1 (MQ=255)                                                                                                                          ttgaGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGGCTGGGCGCCGCGGCAAGCGCTAAAGCCCCCGGTGCACCGggggcggtg  >  2:899044/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                        TGCTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATGGCGCACAGGTGGTGGTG  >  NC_000913/4596302‑4596562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑