Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	347,492	C→T	A318T (GCC→ACC)	prpR ←	DNA‑binding transcriptional dual regulator PrpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	347,492	0	C	T	100.0%	141.7 / NA	45	A318T (GCC→ACC)	prpR	DNA‑binding transcriptional dual regulator PrpR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/22);  total (23/22)

CTACCGGAACAGGCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGAC  >  NC_000913/347366‑347622                                                                                                                               |                                                                                                                                   cTACCGGAACAGGCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGc                                                                                                                       >  1:752908/1‑140 (MQ=255)  tACCGGAACAGGCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGcc                                                                                                                      <  2:478970/140‑1 (MQ=255)   aCCGGAACAGGCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGccc                                                                                                                     <  2:822708/140‑1 (MQ=255)   aCCGGAACAGGCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGccc                                                                                                                     >  1:167470/1‑140 (MQ=255)      ggAACAGGCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGgc                                                                                                                  >  2:90065/1‑140 (MQ=255)             gCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCt                                                                                                           >  2:1044812/1‑140 (MQ=255)             gCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCt                                                                                                           >  1:669382/1‑140 (MQ=255)                gATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCcg                                                                                                        <  1:420893/140‑1 (MQ=255)                       gccgACGCGGGTGACCTCTTTTTNNTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTcgcg                                                                                                 >  2:825199/1‑140 (MQ=255)                       gccgACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTcc                                                                                                         <  1:121281/132‑1 (MQ=255)                       gccgACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTcc                                                                                                         >  2:121281/1‑132 (MQ=255)                       gccgACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTcgcg                                                                                                 >  1:420732/1‑140 (MQ=255)                               gggTGACCTTTTTTTCTCCCAGCCCCCGCAGCGGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCACCCAGAAACAGCGTCCCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTa                                                                                         <  2:420732/140‑1 (MQ=255)                                  tGACCTTTTTTTTTTCCAGCACCCGCAGCGGCCGGGTCTGCAAAGGTGGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAAc                                                                                      <  2:1026778/140‑1 (MQ=255)                                       tctTTTTCTTCCAGCCCCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGcccc                                                                                 <  2:624797/140‑1 (MQ=255)                                           tttCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTcc                                                                             >  1:730889/1‑140 (MQ=255)                                            ttctccCACCCCCCGCAGCAGCCGGGTCTGCAAAGGTGGCGGCATTCCGCCAATCTCATCCAGAAACAGCGTCCCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCt                                                                            <  2:263370/140‑1 (MQ=255)                                            tccttcCAGCCCCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCACCCAAAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCt                                                                            <  2:182151/138‑1 (MQ=255)                                                tcCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATa                                                                        >  1:663833/1‑140 (MQ=255)                                                tcCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATa                                                                        >  1:343809/1‑140 (MQ=255)                                                   aGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGcc                                                                     >  2:368417/1‑140 (MQ=255)                                                   aGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGcc                                                                     <  1:128674/140‑1 (MQ=255)                                                      aCCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTCCCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCaaa                                                                  <  2:573459/140‑1 (MQ=255)                                                      aCCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCaaa                                                                  <  2:570254/140‑1 (MQ=255)                                                       cccGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAAc                                                                 >  2:73490/1‑140 (MQ=255)                                                                    ggTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCTAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGt                                                              <  2:196061/130‑1 (MQ=255)                                                                    ggTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCTAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGt                                                              >  1:196061/1‑130 (MQ=255)                                                                      tCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCcagc                                                  <  1:411586/140‑1 (MQ=255)                                                                        tGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCcagcag                                                <  2:669382/140‑1 (MQ=255)                                                                        tGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCcagcag                                                >  2:902940/1‑140 (MQ=255)                                                                              ggTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTc                                          <  2:752908/140‑1 (MQ=255)                                                                               gTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCg                                         >  1:984826/1‑140 (MQ=255)                                                                                         ttCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGccc                               >  1:82549/1‑140 (MQ=255)                                                                                           cGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCg                             <  2:1023281/140‑1 (MQ=255)                                                                                                     aTCCAGAAACAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACgg                   <  1:368417/140‑1 (MQ=255)                                                                                                           aaaCAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGcaacaa             >  1:215060/1‑140 (MQ=255)                                                                                                           aaaCAGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGcaacaa             >  2:631331/1‑140 (MQ=255)                                                                                                               aGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACaaa            <  2:1031891/137‑1 (MQ=255)                                                                                                               aGCGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACaaa            >  1:1031891/1‑137 (MQ=255)                                                                                                                 cGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGAt       >  1:158527/1‑140 (MQ=255)                                                                                                                 cGTACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTCCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGAt       >  2:218712/1‑140 (MQ=255)                                                                                                                   tACCGCCGTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGc     <  1:90065/140‑1 (MQ=255)                                                                                                                    cccgcggTGGGTAATTTCGAACAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCg    <  2:630352/139‑1 (MQ=255)                                                                                                                    cccgccgTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCg    <  2:675280/139‑1 (MQ=255)                                                                                                                      cgccgTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGAc  <  2:215060/140‑1 (MQ=255)                                                                                                                      cgccgTGGGTAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGAc  >  1:512832/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                   CTACCGGAACAGGCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGAC  >  NC_000913/347366‑347622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑