Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	363,003	G→A	F59F (TTC→TTT)	lacY ←	lactose permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	363,003	0	G	A	100.0%	297.7 / NA	82	F59F (TTC→TTT)	lacY	lactose permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/38);  total (44/38)

ATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGA  >  NC_000913/362872‑363136                                                                                                                                    |                                                                                                                                      aTGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAg                                                                                                                               >  2:735688/1‑140 (MQ=255) aTGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAg                                                                                                                               >  2:886120/1‑140 (MQ=255)     tgtattgtaACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCgag                                                                                                                           <  2:350617/140‑1 (MQ=255)     tgtattgtaACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCgag                                                                                                                           >  2:643378/1‑140 (MQ=255)     tgtattgtaACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCgag                                                                                                                           >  1:350617/1‑140 (MQ=255)      gtattgtaACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAaata                                                                                                                                   >  2:312121/1‑131 (MQ=255)      gtattgtaACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAaata                                                                                                                                   <  1:312121/131‑1 (MQ=255)       tattgtaACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGaa                                                                                                                         <  2:1063/140‑1 (MQ=255)              aCAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGaa                                                                                                                  >  1:561810/1‑140 (MQ=255)                 gTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATa                                                                                                               >  2:245684/1‑140 (MQ=255)                 gTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATa                                                                                                               >  1:812137/1‑140 (MQ=255)                 gTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATa                                                                                                               >  1:899182/1‑140 (MQ=255)                   ggCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGc                                                                                                             >  2:407327/1‑140 (MQ=255)                    gCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCgag                                                                                                                           <  1:800332/125‑1 (MQ=255)                    gCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCgag                                                                                                                           >  2:800332/1‑125 (MQ=255)                    gCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCg                                                                                                            >  1:478767/1‑140 (MQ=255)                    gCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCg                                                                                                            >  1:540843/1‑140 (MQ=255)                    gCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCg                                                                                                            >  1:937950/1‑140 (MQ=255)                    gCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCg                                                                                                            >  2:392768/1‑140 (MQ=255)                      ccGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGc                                                                                                          >  1:916635/1‑140 (MQ=255)                      ccGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGc                                                                                                          >  1:445258/1‑140 (MQ=255)                      ccGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGc                                                                                                          >  1:1042826/1‑140 (MQ=255)                      ccGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGc                                                                                                          <  2:899182/140‑1 (MQ=255)                                aaaTAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAataata                                                                                                <  1:7130/140‑1 (MQ=255)                                aaaTAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAataata                                                                                                >  2:572275/1‑140 (MQ=255)                                   tAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATAccc                                                                                             >  2:289599/1‑140 (MQ=255)                                     aaGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGCACAGAGAAATAGCGGCAAAAataata                                                                                                >  2:924271/1‑135 (MQ=255)                                     aaGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAataata                                                                                                <  1:924271/135‑1 (MQ=255)                                         aCGGCGCAAACATGACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCa                                                                                       <  1:478671/140‑1 (MQ=255)                                          cGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCAc                                                                                      <  2:109111/140‑1 (MQ=255)                                           ggCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACt                                                                                     >  1:1008717/1‑140 (MQ=255)                                              gcAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACtttt                                                                                  <  1:392768/140‑1 (MQ=255)                                               cAAACTTCACTAACATGCCGGTAATAATCCACAGCAGGTACTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTg                                                                                 <  2:937950/140‑1 (MQ=255)                                               cAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTg                                                                                 <  2:540843/140‑1 (MQ=255)                                                    aTCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGat                                                                            <  2:445258/140‑1 (MQ=255)                                                     tCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGata                                                                           <  2:812137/140‑1 (MQ=255)                                                     tCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGata                                                                           <  2:951457/140‑1 (MQ=255)                                                       aCTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATg                                                                         >  2:1045809/1‑140 (MQ=255)                                                         tAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGa                                                                             <  1:770693/134‑1 (MQ=255)                                                         tAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGa                                                                             >  2:770693/1‑134 (MQ=255)                                                         tAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGt                                                                       <  2:916635/140‑1 (MQ=255)                                                         tAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGt                                                                       <  1:245684/140‑1 (MQ=255)                                                         tAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGt                                                                       >  1:174419/1‑140 (MQ=255)                                                         tAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGt                                                                       <  2:1042826/140‑1 (MQ=255)                                                             aTGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGAt                                                                   <  2:252578/140‑1 (MQ=255)                                                             aTGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGAt                                                                   <  1:289599/140‑1 (MQ=255)                                                             aTGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGAt                                                                   <  1:572275/140‑1 (MQ=255)                                                              tGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGa                                                                             <  2:779382/129‑1 (MQ=255)                                                              tGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGa                                                                             >  1:779382/1‑129 (MQ=255)                                                              tGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATg                                                                  >  1:144986/1‑140 (MQ=255)                                                                ccGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGt                                                                       >  1:811160/1‑133 (MQ=255)                                                                ccGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGt                                                                       <  2:811160/133‑1 (MQ=255)                                                                ccGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTc                                                                <  1:1045809/140‑1 (MQ=255)                                                                  ggTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCAt                                                              >  1:1012964/1‑140 (MQ=255)                                                                   gTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATg                                                             <  2:174419/140‑1 (MQ=255)                                                                         atCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCa                                                       >  1:441425/1‑140 (MQ=255)                                                                          tCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCaa                                                      >  2:1009175/1‑140 (MQ=255)                                                                              cagcagGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCg                                                  >  1:695851/1‑140 (MQ=255)                                                                              cagcagGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCg                                                  <  2:1008717/140‑1 (MQ=255)                                                                               agcagGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCgg                                                 >  2:674138/1‑140 (MQ=255)                                                                                 cagGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGa                                               >  1:52550/1‑140 (MQ=255)                                                                                  agGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGaa                                              <  2:144986/140‑1 (MQ=255)                                                                                    gTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGaaaa                                            >  1:312129/1‑140 (MQ=255)                                                                                     tATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGaaaaa                                           <  1:735688/140‑1 (MQ=255)                                                                                     tATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGaa                                              >  2:900156/1‑137 (MQ=255)                                                                                     tATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGaa                                              <  1:900156/137‑1 (MQ=255)                                                                                           cgcAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGa                                      <  2:209264/139‑1 (MQ=255)                                                                                           cgcAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGa                                      >  1:209264/1‑139 (MQ=255)                                                                                            gcAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAg                                    <  1:643378/140‑1 (MQ=255)                                                                                               gCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAg                                 >  1:567220/1‑140 (MQ=255)                                                                                               gCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAg                                 >  2:345102/1‑140 (MQ=255)                                                                                                cccGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAgg                                >  2:693288/1‑140 (MQ=255)                                                                                                             gAAAGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATaaa                   <  1:345102/140‑1 (MQ=255)                                                                                                              aaaGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATaaaa                  >  2:1051935/1‑140 (MQ=255)                                                                                                              aaaGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATaaaa                  >  2:324353/1‑140 (MQ=255)                                                                                                               aaGCAGACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATaaaaa                 >  2:776203/1‑140 (MQ=255)                                                                                                                    gACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCa                          >  1:988993/1‑126 (MQ=255)                                                                                                                    gACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCa                          <  2:988993/126‑1 (MQ=255)                                                                                                                    gACCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTaa            >  1:651970/1‑140 (MQ=255)                                                                                                                     aCCAAACAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTaaa           <  2:478767/140‑1 (MQ=255)                                                                                                                          aCAGCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTaaaagaaa      <  2:1012964/140‑1 (MQ=255)                                                                                                                             gCGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTaaaagaaaaag   <  1:1051935/140‑1 (MQ=255)                                                                                                                              cGGTTGAAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTaaaagaaaaaga  <  2:567220/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      ATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGA  >  NC_000913/362872‑363136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑