Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	55,489	A→G	F541L (TTC→CTC)	lptD ←	lipopolysaccharide assembly protein LptD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	55,489	0	A	G	100.0%	301.2 / NA	81	F541L (TTC→CTC)	lptD	lipopolysaccharide assembly protein LptD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/45);  total (36/45)

CGTGAAATAGTAGATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCA  >  NC_000913/55352‑55620                                                                                                                                          |                                                                                                                                    cGTGAAATAGTAGATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCa                                                                                                                                   >  1:448797/1‑140 (MQ=255) cGTGAAATAGTAGATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCa                                                                                                                                   >  1:637572/1‑140 (MQ=255) cGTGAAATAGTAGATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCa                                                                                                                                   >  1:761975/1‑140 (MQ=255)   tGAAATAGTAGATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAgg                                                                                                                                 <  1:105217/140‑1 (MQ=255)   tGAAATAGTAGATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAgg                                                                                                                                 >  2:86273/1‑140 (MQ=255)   tGAAATAGTAGATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAgg                                                                                                                                 <  1:789806/140‑1 (MQ=255)      aaTAGTAGATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCa                                                                                                                              >  2:277599/1‑140 (MQ=255)             gATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCCCCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGagtagt                                                                                                                       <  2:864508/140‑1 (MQ=255)             gATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGagtagt                                                                                                                       <  2:909386/140‑1 (MQ=255)             gATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGagtagt                                                                                                                       >  2:873196/1‑140 (MQ=255)               tttGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCa                                                                                                                     <  1:277599/140‑1 (MQ=255)               tttGACCAACGAAAATATAAAAACGTTCAACGGCAGCTTCATCATATAGGCGAGATGTGACGCCGGTCGTCACCTGTTTAGCGGAGCAAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCa                                                                                                                     <  2:761975/140‑1 (MQ=255)                ttGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAg                                                                                                                    >  1:451643/1‑140 (MQ=255)                  gACCAAGAGAAATATTAAAGTGTTCAACGGCGGCATCATCATATATGCGAGATGTGACACCGGTCGTCACTTGGGTGGGGGAGGCAATACGGTAAAGCCCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGAt                                                                                                                  <  2:404148/140‑1 (MQ=255)                  gACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAgg                                                                                                                                 >  1:979355/1‑125 (MQ=255)                  gACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAgg                                                                                                                                 <  2:979355/125‑1 (MQ=255)                  gACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGAt                                                                                                                  <  1:11623/140‑1 (MQ=255)                  gACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGAt                                                                                                                  >  1:576699/1‑140 (MQ=255)                        cGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTgcagc                                                                                                            >  1:344366/1‑140 (MQ=255)                             atatTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCCCCTGGTTAGCGGAGGCAATACGGTCAATACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAgaga                                                                                                       <  1:873940/140‑1 (MQ=255)                                  aaaaCGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCCCCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGt                                                                                                  <  1:391901/140‑1 (MQ=255)                                  aaaaCGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGt                                                                                                  <  1:14156/140‑1 (MQ=255)                                  aaaaCGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGt                                                                                                  <  1:172594/140‑1 (MQ=255)                                  aaaaCGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGt                                                                                                  <  2:448797/140‑1 (MQ=255)                                  aaaaCGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGt                                                                                                  <  1:622853/140‑1 (MQ=255)                                   aaaCGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTc                                                                                                 <  1:195698/140‑1 (MQ=255)                                     aCGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGt                                                                                               <  2:344366/140‑1 (MQ=255)                                        ttCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCTAGAGTAGTCAGATTGCAGCagag                                                                                                        <  1:373544/128‑1 (MQ=255)                                        ttCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCagag                                                                                                        >  2:373544/1‑128 (MQ=255)                                         tCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGtt                                                                                           >  1:523996/1‑140 (MQ=255)                                           aaCGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTtat                                                                                         <  1:519199/140‑1 (MQ=255)                                                  gcaTCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCg                                                                                                >  2:851327/1‑126 (MQ=255)                                                  gcaTCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCg                                                                                                <  1:851327/126‑1 (MQ=255)                                                     tcatcatATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGct                                                                               <  1:873196/140‑1 (MQ=255)                                                             tatGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATcgc                                                                       >  1:312611/1‑140 (MQ=255)                                                                gCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGAt                                                                    >  2:818481/1‑140 (MQ=255)                                                                gCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGAt                                                                    >  1:933717/1‑140 (MQ=255)                                                                gCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGAt                                                                    >  1:869058/1‑140 (MQ=255)                                                                gCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGAt                                                                    <  1:856764/140‑1 (MQ=255)                                                                   agaTGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACg                                                                 <  1:224223/140‑1 (MQ=255)                                                                   agaTGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACg                                                                 <  2:576699/140‑1 (MQ=255)                                                                   agaTGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACg                                                                 >  2:57038/1‑140 (MQ=255)                                                                    gaTGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACgg                                                                <  2:933717/140‑1 (MQ=255)                                                                    gaTGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACgg                                                                >  2:336243/1‑140 (MQ=255)                                                                    gaTGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAACACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACgg                                                                <  2:590498/140‑1 (MQ=255)                                                                     aTGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGc                                                               >  1:724236/1‑140 (MQ=255)                                                                      tgtgCCCCCGTTCGTCCCCTGGTTAGCGGAGGCAATACGTTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCa                                                              <  2:312611/140‑1 (MQ=255)                                                                      tgtgACCCGGGTCGTCACCTGGTTAGCGGAGGAAATACGGTAAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCa                                                              <  2:523996/140‑1 (MQ=255)                                                                      tgtgACCCCGGTCGTCACCTGTTTAGCGGAGGAAATACGGTAAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCa                                                              <  1:261028/140‑1 (MQ=255)                                                                      tgtgACACCGGTCGTCCCCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCa                                                              <  1:740283/140‑1 (MQ=255)                                                                      tgtgACACCGGTCGTCCCCTGGTTAGCGGAGCCAATGCGGTCAAGTCCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCa                                                              <  2:676430/140‑1 (MQ=255)                                                                      tgtgACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCa                                                              <  2:75081/140‑1 (MQ=255)                                                                      tgtgACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCa                                                              >  2:178559/1‑140 (MQ=255)                                                                      tgtgACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCa                                                              <  1:57038/140‑1 (MQ=255)                                                                      tgtgACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCa                                                              <  2:96418/140‑1 (MQ=255)                                                                        tgACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACg                                                            <  1:424103/140‑1 (MQ=255)                                                                         gACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGt                                                           >  1:732293/1‑140 (MQ=255)                                                                               ggTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAAt                                                     >  1:696564/1‑140 (MQ=255)                                                                                gtcgtcACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCTCGGAGCAGGCCAGTGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATa                                                    >  2:408245/1‑140 (MQ=255)                                                                                gtcgtcACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATa                                                    >  2:843423/1‑140 (MQ=255)                                                                                gtcgtcACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATa                                                    >  1:905990/1‑140 (MQ=255)                                                                                gtcgtcACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATa                                                    >  2:478392/1‑140 (MQ=255)                                                                                         tGGTTGGGGGGGCCATTACGGTCAATACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTgcgcgcg                                           <  2:266839/140‑1 (MQ=255)                                                                                         tGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTgcgcgcg                                           <  1:478392/140‑1 (MQ=255)                                                                                         tGGTTAGCGGAGGAACTGCGGTCAGGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTgcgcgcg                                           <  2:925839/140‑1 (MQ=255)                                                                                           gTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTAc                                                         <  1:833701/124‑1 (MQ=255)                                                                                           gTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTAc                                                         >  2:833701/1‑124 (MQ=255)                                                                                              aGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGtt                                      >  1:925839/1‑140 (MQ=255)                                                                                                cGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTcc                                    <  2:696564/140‑1 (MQ=255)                                                                                                     gCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGt                               >  1:549917/1‑140 (MQ=255)                                                                                                     gCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGt                               >  2:374507/1‑140 (MQ=255)                                                                                                      cAATCCGGTCAAGACCGCCGTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGGCTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGtt                              <  2:451643/140‑1 (MQ=255)                                                                                                                       ccgTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGc             >  2:89810/1‑140 (MQ=255)                                                                                                                       ccgTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGc             >  2:923330/1‑140 (MQ=255)                                                                                                                        cgTAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTACCAGCGTTTGGGTATAACCCGGAGcc            <  1:336243/140‑1 (MQ=255)                                                                                                                          tAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAg          <  2:869058/140‑1 (MQ=255)                                                                                                                          tAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAg          >  1:644614/1‑140 (MQ=255)                                                                                                                          tAAGTCCGGTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAg          <  1:86273/140‑1 (MQ=255)                                                                                                                                 ggTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTcc   >  1:32288/1‑140 (MQ=255)                                                                                                                                 ggTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTcc   >  2:161166/1‑140 (MQ=255)                                                                                                                                 ggTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTcc   <  2:32288/140‑1 (MQ=255)                                                                                                                                  gTCCCGGAGCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCa  >  2:820122/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    CGTGAAATAGTAGATTTGACCAACGGAAATATTAAAACGTTCAACGGCAGCATCATCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCA  >  NC_000913/55352‑55620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑