Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	603,108	A→G	M384V (ATG→GTG)	pheP →	phenylalanine:H(+) symporter PheP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	603,108	0	A	G	100.0%	166.8 / NA	46	M384V (ATG→GTG)	pheP	phenylalanine:H(+) symporter PheP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/24);  total (22/24)

GGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTG  >  NC_000913/602973‑603244                                                                                                                                        |                                                                                                                                         ggTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGAt                                                                                                                                      <  2:615783/140‑1 (MQ=255)         gATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTgg                                                                                                                              <  2:160983/140‑1 (MQ=255)               cTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGAGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATc                                                                                                                        >  1:75026/1‑140 (MQ=255)                 cGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTg                                                                                                                      <  2:265046/140‑1 (MQ=255)                   cTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCg                                                                                                                    <  2:396655/140‑1 (MQ=255)                           ttCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTg                                                                                                            <  2:449620/140‑1 (MQ=255)                            tCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGc                                                                                                           >  1:416016/1‑140 (MQ=255)                                  gCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGAt                                                                                                     >  2:538707/1‑140 (MQ=255)                                     aTCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCg                                                                                                  >  1:335196/1‑140 (MQ=255)                                      tCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGa                                                                                                 >  1:362494/1‑140 (MQ=255)                                            cGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAg                                                                                           <  2:765445/140‑1 (MQ=255)                                                    gtgtTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGAt                                                                                                     >  1:297172/1‑122 (MQ=255)                                                    gtgtTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGAt                                                                                                     <  2:297172/122‑1 (MQ=255)                                                    gtgtTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGa                                                                                   >  1:477069/1‑140 (MQ=255)                                                      gtTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGaaa                                                                                 >  2:318634/1‑140 (MQ=255)                                                       ttAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAac                                                                                <  2:579631/140‑1 (MQ=255)                                                            cAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGt                                                                           <  2:114684/140‑1 (MQ=255)                                                               cTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTa                                                                        >  1:640894/1‑140 (MQ=255)                                                                 aTCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAg                                                                      >  2:452977/1‑140 (MQ=255)                                                                  tCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAgg                                                                     <  1:857329/140‑1 (MQ=255)                                                                    tgctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   <  2:131844/140‑1 (MQ=255)                                                                    tgctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   >  2:133106/1‑140 (MQ=255)                                                                    tgctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   <  2:477069/140‑1 (MQ=255)                                                                    tgctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   <  2:818786/140‑1 (MQ=255)                                                                     gctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgc                                                                    <  2:1000784/138‑1 (MQ=255)                                                                     gctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgc                                                                    >  1:1000784/1‑138 (MQ=255)                                                                       tgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   <  1:878897/137‑1 (MQ=255)                                                                       tgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   >  2:878897/1‑137 (MQ=255)                                                                              aaaaGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATc                                                         <  1:452977/140‑1 (MQ=255)                                                                                    ctttGGTGTGCTGATGGCGCGGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGGCGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCg                                                   <  2:640894/139‑1 (MQ=255)                                                                                     tttGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCgg                                                  <  1:195180/140‑1 (MQ=255)                                                                                          tCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACt                                             >  1:1028469/1‑140 (MQ=255)                                                                                             gctgATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTAtc                                          >  2:1038900/1‑140 (MQ=255)                                                                                              ctgATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTAtct                                         <  1:32051/140‑1 (MQ=255)                                                                                                   ggCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCg                                                   >  1:528548/1‑125 (MQ=255)                                                                                                   ggCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTg                                      <  1:1010776/138‑1 (MQ=255)                                                                                                   ggCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTg                                      >  2:1010776/1‑138 (MQ=255)                                                                                                   ggCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCa                                    >  1:59370/1‑140 (MQ=255)                                                                                                   ggCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTCCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCg                                                   <  2:528548/125‑1 (MQ=255)                                                                                                      gcTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTg                                 <  1:287114/140‑1 (MQ=255)                                                                                                           tggtAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTc                            >  1:386068/1‑140 (MQ=255)                                                                                                                    cGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATg                   >  2:956378/1‑140 (MQ=255)                                                                                                                     gctgctGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGa                  >  2:105805/1‑140 (MQ=255)                                                                                                                      ctgctgTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGAt                 <  1:318634/140‑1 (MQ=255)                                                                                                                        gctgTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGAttt               <  1:824119/140‑1 (MQ=255)                                                                                                                                    gATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGAtgt   >  2:97994/1‑140 (MQ=255)                                                                                                                                     aTTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGAtgtg  <  2:940432/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTG  >  NC_000913/602973‑603244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑