Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	615,673	A→G	D506G (GAC→GGC)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	615,673	0	A	G	100.0%	133.7 / NA	37	D506G (GAC→GGC)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/22);  total (15/22)

ACAAGCGGCAAAAACACCGGATGCTCCGGCGCTGGCAGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGAT  >  NC_000913/615545‑615806                                                                                                                                 |                                                                                                                                      aCAAGCGGCAAAAACACCGGATGCTCCGGCGCTGGCAGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGTGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCgg                                                                                                                            <  1:96194/140‑1 (MQ=255) aCAAGCGGCAAAAACACCGGATGCTCCGGCGCTGGCAGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCgg                                                                                                                            >  1:320593/1‑140 (MQ=255)   aaGCGGCAAAAACACCGGATGCTCCGGCGCTGGCAGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTg                                                                                                                          >  2:83956/1‑140 (MQ=255)   aaGCGGCAAAAACACCGGATGCTCCGGCGCTGGCAGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTg                                                                                                                          >  2:66423/1‑140 (MQ=255)    aGCGGCAAAAACACCGGATGCTCCGGCGCTGGCAGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTgg                                                                                                                         <  1:603577/140‑1 (MQ=255)                   ggATGCTCCGGCGCTGGCAGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCgg                                                                                                          >  1:326441/1‑140 (MQ=255)                        cTCCGGCGCTGGCAGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCGGGGGGCAGCGTGGCGGTGACACTACCGCGCTCGGTCttt                                                                                                     <  2:532515/140‑1 (MQ=255)                              cgcTGGCAGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGAcc                                                                                               <  2:509178/140‑1 (MQ=255)                                     aGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGa                                                                                                 <  2:837078/131‑1 (MQ=255)                                     aGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGa                                                                                                 <  2:717062/131‑1 (MQ=255)                                     aGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGa                                                                                                 >  1:717062/1‑131 (MQ=255)                                     aGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGa                                                                                                 >  1:837078/1‑131 (MQ=255)                                             gTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCg                                                                                <  1:5770/140‑1 (MQ=255)                                             gTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCg                                                                                <  1:83956/140‑1 (MQ=255)                                              ttACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGa                                                                               <  1:940065/140‑1 (MQ=255)                                                aCCTGTTCAGCTATCGGGAAATGTGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATa                                                                             <  1:172427/140‑1 (MQ=255)                                                          cTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCa                                                                                    >  2:384218/1‑123 (MQ=255)                                                          cTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCa                                                                                    <  1:384218/123‑1 (MQ=255)                                                          cTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTg                                                                   >  1:589334/1‑140 (MQ=255)                                                           tATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTTGCACTCCATGCGGTAGTTTAAGCTgg                                                                  >  2:716332/1‑140 (MQ=255)                                                                 gAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGc                                                            <  1:535002/140‑1 (MQ=255)                                                                    aTGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTg                                                         >  1:1045509/1‑140 (MQ=255)                                                                    aTGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTg                                                         <  2:326441/140‑1 (MQ=255)                                                                       cgcgAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCt                                                      >  1:661339/1‑140 (MQ=255)                                                                        gcgAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTa                                                     <  2:589334/140‑1 (MQ=255)                                                                         cgAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTAc                                                    >  1:249719/1‑140 (MQ=255)                                                                           aGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCg                                                  <  1:716332/140‑1 (MQ=255)                                                                           aGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCg                                                  <  1:66423/140‑1 (MQ=255)                                                                                      gcgcTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCgg                                       >  1:783600/1‑140 (MQ=255)                                                                                      gcgcTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCgg                                       <  1:193323/140‑1 (MQ=255)                                                                                      gcgcTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCgg                                       >  2:668698/1‑140 (MQ=255)                                                                                      gcgcTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCgg                                       <  2:320593/140‑1 (MQ=255)                                                                                      gcgcTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCgg                                       <  1:827773/140‑1 (MQ=255)                                                                                                       gcGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCt                                     >  1:956984/1‑125 (MQ=255)                                                                                                       gcGTGAGCGCGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCt                                     <  2:956984/125‑1 (MQ=255)                                                                                                             gcgcGGCGTTAAACCAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGaaaa                <  2:661339/140‑1 (MQ=255)                                                                                                                           cAGGGGGCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGAt  <  2:1045509/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      ACAAGCGGCAAAAACACCGGATGCTCCGGCGCTGGCAGATGCGCGTTACCTGTTCAGCTATCGGGAAATGCGCGAGCAGGTGGTGGCGCTGGCGAATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGAT  >  NC_000913/615545‑615806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑