Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	951,980	A→G	C525C (TGT→TGC)	pflB ←	pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	951,980	0	A	G	100.0%	293.5 / NA	79	C525C (TGT→TGC)	pflB	pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/46);  total (33/46)

ATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGA  >  NC_000913/951845‑952112                                                                                                                                        |                                                                                                                                     aTTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACg                                                                                                                                  >  2:234131/1‑140 (MQ=255)  ttGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGc                                                                                                                                 <  2:1044005/140‑1 (MQ=255)   tGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGcc                                                                                                                                <  1:234131/140‑1 (MQ=255)      tACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATg                                                                                                                             >  1:645100/1‑140 (MQ=255)      tACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATg                                                                                                                             >  1:872568/1‑140 (MQ=255)       aCCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCa                                                                                                                               >  1:321589/1‑137 (MQ=255)       aCCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCa                                                                                                                               <  2:321589/137‑1 (MQ=255)        ccAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGt                                                                                                                           >  2:48033/1‑140 (MQ=255)        ccAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGt                                                                                                                           >  2:13341/1‑140 (MQ=255)        ccAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGt                                                                                                                           >  1:506107/1‑140 (MQ=255)         cAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTg                                                                                                                          <  1:983534/140‑1 (MQ=255)          aaaCTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACg                                                                                                                                  >  1:552006/1‑131 (MQ=255)          aaaCTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACg                                                                                                                                  <  2:552006/131‑1 (MQ=255)             cTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGa                                                                                                                      >  1:516538/1‑140 (MQ=255)              tGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGAt                                                                                                                     <  1:52428/140‑1 (MQ=255)              tGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGAt                                                                                                                     <  2:506107/140‑1 (MQ=255)              tGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGAt                                                                                                                     <  2:615563/140‑1 (MQ=255)                 gggTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATTTTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAAc                                                                                                                  >  1:428620/1‑140 (MQ=255)                     aTTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCa                                                                                                              <  2:233445/140‑1 (MQ=255)                        cGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACgg                                                                                                           >  1:978181/1‑140 (MQ=255)                            ttCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGt                                                                                                       <  2:789703/140‑1 (MQ=255)                              cGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGc                                                                                                     <  2:327311/140‑1 (MQ=255)                              cGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGc                                                                                                     >  1:772382/1‑140 (MQ=255)                                aTTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAg                                                                                                   >  1:266116/1‑140 (MQ=255)                                aTTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAg                                                                                                   <  2:403673/140‑1 (MQ=255)                                 tttCGAAGTCGATAGCCAGACCGTCCTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAgc                                                                                                  <  2:978181/140‑1 (MQ=255)                                         tCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCag                                                                                          >  2:52738/1‑140 (MQ=255)                                                 cAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                                                                  <  1:427033/140‑1 (MQ=255)                                                   gACCGTCTTCGTCACGAATCGGTTTAACTTGCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGGCAGACCAGCGATGCCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                                                                <  2:973799/140‑1 (MQ=255)                                                     ccGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTa                                                                              >  2:802802/1‑140 (MQ=255)                                                       gTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGc                                                                            <  2:655531/140‑1 (MQ=255)                                                        tCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCt                                                                           >  1:518399/1‑140 (MQ=255)                                                         cTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                                                                          >  1:749891/1‑140 (MQ=255)                                                          ttCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGt                                                                         >  2:357191/1‑140 (MQ=255)                                                           tCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                                                                        <  1:616412/140‑1 (MQ=255)                                                            cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTAc                                                                       <  2:266116/140‑1 (MQ=255)                                                                   aaTCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATGCCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt                                                                <  2:275472/140‑1 (MQ=255)                                                                   aaTCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt                                                                <  1:357191/140‑1 (MQ=255)                                                                   aaTCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt                                                                <  2:22414/140‑1 (MQ=255)                                                                      cGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCa                                                             <  1:1045917/140‑1 (MQ=255)                                                                        gTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATg                                                           >  2:408933/1‑140 (MQ=255)                                                                            aaCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGt                                                       <  1:13341/140‑1 (MQ=255)                                                                              cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTgg                                                     <  2:172259/140‑1 (MQ=255)                                                                               tttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGga                                                    <  1:923212/140‑1 (MQ=255)                                                                               tttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGga                                                    <  1:264393/140‑1 (MQ=255)                                                                                 tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatg                                                  <  1:537983/140‑1 (MQ=255)                                                                                  cGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatga                                                 >  1:608570/1‑140 (MQ=255)                                                                                  cGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatg                                                  >  1:867267/1‑139 (MQ=255)                                                                                  cGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatg                                                  <  2:867267/139‑1 (MQ=255)                                                                                   gCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatgat                                                >  1:436946/1‑140 (MQ=255)                                                                                   gCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatgat                                                >  2:416588/1‑140 (MQ=255)                                                                                      tatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGtt                                             <  2:772382/140‑1 (MQ=255)                                                                                      tatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGtt                                             <  2:518399/140‑1 (MQ=255)                                                                                       atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTc                                            >  2:478619/1‑140 (MQ=255)                                                                                        tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTca                                           <  2:608570/140‑1 (MQ=255)                                                                                        tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTca                                           <  1:48033/140‑1 (MQ=255)                                                                                           gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg                                        <  1:660430/140‑1 (MQ=255)                                                                                           gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg                                        <  1:403189/140‑1 (MQ=255)                                                                                              tGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcag                                     >  2:1059826/1‑140 (MQ=255)                                                                                                   aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATg                                <  2:872568/140‑1 (MQ=255)                                                                                                    cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGt                               <  1:802802/140‑1 (MQ=255)                                                                                                       ggAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACt                            >  1:200187/1‑140 (MQ=255)                                                                                                          gTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                         <  1:52738/140‑1 (MQ=255)                                                                                                          gTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                         >  2:275979/1‑140 (MQ=255)                                                                                                             agcagcAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcag                                     <  2:786614/125‑1 (MQ=255)                                                                                                             agcagcAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcag                                     >  1:786614/1‑125 (MQ=255)                                                                                                             agcagcAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTag                      >  1:100050/1‑140 (MQ=255)                                                                                                                 gcaACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccag                  <  2:200187/140‑1 (MQ=255)                                                                                                                   aaCGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagcc                <  1:1059826/140‑1 (MQ=255)                                                                                                                   aaCGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagcc                <  1:686528/140‑1 (MQ=255)                                                                                                                   aaCGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagcc                <  1:408933/140‑1 (MQ=255)                                                                                                                    aCGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAgccagcca               <  2:516538/140‑1 (MQ=255)                                                                                                                        aCAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTcc           <  2:749891/140‑1 (MQ=255)                                                                                                                        aCAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTcc           <  1:572763/140‑1 (MQ=255)                                                                                                                         cAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCa          <  2:804324/140‑1 (MQ=255)                                                                                                                            aCCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGa       >  2:871533/1‑140 (MQ=255)                                                                                                                            aCCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGa       >  1:225342/1‑140 (MQ=255)                                                                                                                                 cGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa  >  2:783337/1‑140 (MQ=255)                                                                                                                                 cGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa  >  2:969599/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     ATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGA  >  NC_000913/951845‑952112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑