Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,670	A→G	R284R (CGT→CGC)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,670	0	A	G	100.0%	300.0 / NA	80	R284R (CGT→CGC)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/33);  total (47/33)

CCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGG  >  NC_000913/73534‑73800                                                                                                                                         |                                                                                                                                   ccccccACAGCCTGGGTTGTGCCAGCGCTCCCGGCAACGGTATTTTCCCCCCACCGACTATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGa                                                                                                                                 <  2:173527/136‑1 (MQ=255) ccTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGa                                                                                                                                 >  1:547950/1‑140 (MQ=255)    gCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCg                                                                                                                              >  1:885370/1‑140 (MQ=255)    gCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCg                                                                                                                              >  1:685368/1‑140 (MQ=255)          ccccgggTTGTGCCAGCTTTCCGGGCATCTGGTGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCACAAATGCGGCTATGCAGGCGATCGTCCggg                                                                                                                        <  1:741061/135‑1 (MQ=255)              cGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTcgc                                                                                                                    >  1:27261/1‑140 (MQ=255)                gTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCgcgc                                                                                                                  >  1:187089/1‑140 (MQ=255)                gTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCgcgc                                                                                                                  >  1:747325/1‑140 (MQ=255)                gTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCgcgc                                                                                                                  >  1:540609/1‑140 (MQ=255)                 ttGTGCCAGCCCTCCCGGCAACTGGCGTTTTCCCCCACCGACGACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGcc                                                                                                                 <  2:481016/140‑1 (MQ=255)                      ccAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCt                                                                                                            >  1:832261/1‑140 (MQ=255)                        aGCACTCCCGGCACCTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTg                                                                                                          <  1:701558/140‑1 (MQ=255)                          cACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCTGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTgca                                                                                                        >  2:1002088/1‑140 (MQ=255)                          cACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTgca                                                                                                        >  1:240993/1‑140 (MQ=255)                                cGGCACCTGGCGATTCCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCg                                                                                                  <  2:160507/140‑1 (MQ=255)                                          cttttccccccATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCggg                                                                                        <  2:973761/133‑1 (MQ=255)                                                 ccccATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATg                                                                                 >  2:852037/1‑140 (MQ=255)                                                  cccATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATgg                                                                                <  1:852037/140‑1 (MQ=255)                                                       cgacgaTCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTa                                                                           >  1:84938/1‑140 (MQ=255)                                                       cgacgaTCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTa                                                                           >  1:585544/1‑140 (MQ=255)                                                                ccGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCaa                                                                                   <  1:943859/123‑1 (MQ=255)                                                                ccGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATGAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCaa                                                                                   >  2:943859/1‑123 (MQ=255)                                                                 cGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCt                                                                 <  2:17365/140‑1 (MQ=255)                                                                 cGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCt                                                                 >  1:206283/1‑140 (MQ=255)                                                                   ccAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGa                                                               <  2:153619/140‑1 (MQ=255)                                                                    cAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGAc                                                              <  2:1002270/140‑1 (MQ=255)                                                                       tAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCa                                                           >  2:123439/1‑140 (MQ=255)                                                                       tAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCa                                                           >  2:592666/1‑140 (MQ=255)                                                                       tAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCa                                                           >  2:761760/1‑140 (MQ=255)                                                                          cGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGCGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACa                                                        >  2:277597/1‑140 (MQ=255)                                                                           ggTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAg                                                       >  2:486120/1‑140 (MQ=255)                                                                            gTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGAc                                                              <  2:548135/132‑1 (MQ=255)                                                                            gTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGAc                                                              >  1:548135/1‑132 (MQ=255)                                                                              ggCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCAc                                                    <  1:367366/140‑1 (MQ=255)                                                                              ggCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCAc                                                    >  1:472246/1‑140 (MQ=255)                                                                               gcagcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCAcc                                                   >  2:31678/1‑140 (MQ=255)                                                                               gcagcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCAcc                                                   >  1:317209/1‑140 (MQ=255)                                                                                cagcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCa                                                  >  1:445906/1‑140 (MQ=255)                                                                                   caACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGcc                                               >  2:38828/1‑140 (MQ=255)                                                                                    aaCAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGccc                                              >  2:824929/1‑140 (MQ=255)                                                                                     aCAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCg                                             >  2:924199/1‑140 (MQ=255)                                                                                        gcagcagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAgg                                          <  1:123439/140‑1 (MQ=255)                                                                                         cagcagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAgg                                          <  1:776274/139‑1 (MQ=255)                                                                                         cagcagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAgg                                          >  2:776274/1‑139 (MQ=255)                                                                                            cagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGgcagc                                      <  1:761760/140‑1 (MQ=255)                                                                                            cagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGgcagc                                      <  1:34481/140‑1 (MQ=255)                                                                                            cagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGgcagc                                      <  1:326799/140‑1 (MQ=255)                                                                                            cagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGgcagc                                      <  1:592666/140‑1 (MQ=255)                                                                                              gcgcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcac                                    <  2:585544/140‑1 (MQ=255)                                                                                               cgcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcaca                                   <  2:187089/140‑1 (MQ=255)                                                                                               cgcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcaca                                   <  1:936625/140‑1 (MQ=255)                                                                                               cgcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcaca                                   <  1:550059/140‑1 (MQ=255)                                                                                               cgcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcaca                                   <  1:38828/140‑1 (MQ=255)                                                                                                 ccAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACAcc                                 >  1:924333/1‑140 (MQ=255)                                                                                                 ccAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACAcc                                 >  2:916025/1‑140 (MQ=255)                                                                                                    gCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATc                              >  1:513916/1‑140 (MQ=255)                                                                                                    gCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATc                              <  2:472246/140‑1 (MQ=255)                                                                                                         aTTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGag                         <  1:486120/140‑1 (MQ=255)                                                                                                         aTTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGag                         <  2:513916/140‑1 (MQ=255)                                                                                                         aTTAACACCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGag                         <  1:240524/140‑1 (MQ=255)                                                                                                               acCGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAgcgc                   >  1:873618/1‑140 (MQ=255)                                                                                                                ccGTGTCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGcc                  >  2:993673/1‑140 (MQ=255)                                                                                                                  gtgtCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAg                >  1:384445/1‑140 (MQ=255)                                                                                                                  gtgtCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAg                >  1:743634/1‑140 (MQ=255)                                                                                                                  gtgtCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAg                >  1:231544/1‑140 (MQ=255)                                                                                                                   tgtCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGc               >  1:997525/1‑140 (MQ=255)                                                                                                                   tgtCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGc               >  1:561939/1‑140 (MQ=255)                                                                                                                   tgtCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGc               >  2:1014566/1‑140 (MQ=255)                                                                                                                   tgtCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGc               >  2:161697/1‑140 (MQ=255)                                                                                                                    gtCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCa              >  2:121198/1‑140 (MQ=255)                                                                                                                    gtCGCAAATGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCa              >  2:303829/1‑140 (MQ=255)                                                                                                                           aTGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgc       >  1:385727/1‑140 (MQ=255)                                                                                                                           aTGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgc       >  1:853996/1‑140 (MQ=255)                                                                                                                           aTGCGGCTATGCAGGCAATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgc       >  2:757211/1‑140 (MQ=255)                                                                                                                            tGCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgcg      <  2:231544/140‑1 (MQ=255)                                                                                                                             gCGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAACCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgcgc     <  2:206283/140‑1 (MQ=255)                                                                                                                              cGGCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgcgcg    >  1:644257/1‑140 (MQ=255)                                                                                                                                gCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCggg  <  2:743634/140‑1 (MQ=255)                                                                                                                                gCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCggg  <  2:547950/140‑1 (MQ=255)                                                                                                                                gCTATGCAGGCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCggg  <  2:384445/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGG  >  NC_000913/73534‑73800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑