Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,074,478	A→G	A79A (GCA→GCG)	rutR →	DNA‑binding transcriptional dual regulator RutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,074,478	0	A	G	100.0%	172.2 / NA	46	A79A (GCA→GCG)	rutR	DNA‑binding transcriptional dual regulator RutR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/22);  total (24/22)

TTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCG  >  NC_000913/1074341‑1074613                                                                                                                                          |                                                                                                                                        ttCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGcc                                                                                                                                       >  1:936679/1‑140 (MQ=255)                               aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGcccc                                                                                                         >  1:282484/1‑140 (MQ=255)                               aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGcccc                                                                                                         >  1:690005/1‑140 (MQ=255)                               aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGcccc                                                                                                         >  1:417936/1‑140 (MQ=255)                               aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGcccc                                                                                                         >  2:564279/1‑140 (MQ=255)                               aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGc                                                                                                            >  2:499650/1‑137 (MQ=255)                               aTCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGc                                                                                                            <  1:499650/137‑1 (MQ=255)                                   cAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTg                                                                                                     >  1:438950/1‑140 (MQ=255)                                   cAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTg                                                                                                     >  1:975689/1‑140 (MQ=255)                                    agagTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTgg                                                                                                    >  2:44798/1‑140 (MQ=255)                                          ggCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGa                                                                                              >  1:379011/1‑140 (MQ=255)                                          ggCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGa                                                                                              >  1:352107/1‑140 (MQ=255)                                           gCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTggc                                                                                                   <  2:796846/134‑1 (MQ=255)                                           gCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTggc                                                                                                   >  1:796846/1‑134 (MQ=255)                                             gggTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCa                                                                                           <  1:550078/140‑1 (MQ=255)                                             gggTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCa                                                                                           <  2:417936/140‑1 (MQ=255)                                             gggTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCa                                                                                           <  2:282484/140‑1 (MQ=255)                                                tgtTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGAt                                                                                             >  1:27377/1‑135 (MQ=255)                                                tgtTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGAt                                                                                             <  2:27377/135‑1 (MQ=255)                                                  tttCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAagag                                                                                      >  1:591630/1‑140 (MQ=255)                                                      aaaaaCCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACa                                                                                  <  2:980358/140‑1 (MQ=255)                                                                    tgTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCtt                                                                    >  1:313624/1‑139 (MQ=255)                                                                       aTTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGaa                                                                 <  1:820279/140‑1 (MQ=255)                                                                                cGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCAcgc                                                        >  2:967723/1‑140 (MQ=255)                                                                                cGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCAcgc                                                        >  1:586694/1‑140 (MQ=255)                                                                                  tCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGa                                                      >  1:374213/1‑140 (MQ=255)                                                                                  tCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGa                                                      >  2:435010/1‑140 (MQ=255)                                                                                           gcgcTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCa                                             <  1:967723/140‑1 (MQ=255)                                                                                                gTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCtt                                        <  1:435010/140‑1 (MQ=255)                                                                                                     ttGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCgcgc                                   <  2:379011/140‑1 (MQ=255)                                                                                                     ttGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCgcgc                                   <  2:352107/140‑1 (MQ=255)                                                                                                     ttGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCgcgc                                   <  2:505367/140‑1 (MQ=255)                                                                                                       gCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCt                                 <  2:656161/140‑1 (MQ=255)                                                                                                          gTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGtt                              <  1:962455/140‑1 (MQ=255)                                                                                                          gTGCTGCGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGtt                              <  2:690005/140‑1 (MQ=255)                                                                                                           tgctgcGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGtt                              >  1:639423/1‑139 (MQ=255)                                                                                                           tgctgcGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGtt                              <  2:639423/139‑1 (MQ=255)                                                                                                           tgctgcGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTc                             >  1:950657/1‑140 (MQ=255)                                                                                                           tgctgcGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTc                             >  1:768785/1‑140 (MQ=255)                                                                                                            gctgcGGCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCt                            <  1:564279/140‑1 (MQ=255)                                                                                                                  gCAGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATgg                      >  2:145888/1‑140 (MQ=255)                                                                                                                    aGATTCTCGATATCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGgag                    <  2:591630/140‑1 (MQ=255)                                                                                                                                tCTGGCTGGCGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCGGgc        >  1:414537/1‑140 (MQ=255)                                                                                                                                      tggcGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGcgccg  <  2:594326/140‑1 (MQ=255)                                                                                                                                      tggcGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGcgccg  <  1:786718/140‑1 (MQ=255)                                                                                                                                      tggcGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGcgccg  <  2:950657/140‑1 (MQ=255)                                                                                                                                      tggcGCCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGcgccg  <  2:975689/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATATCTGGCTGGCACCGTTAAAAGCGTTTCGTGAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCG  >  NC_000913/1074341‑1074613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑