Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,075,403	A→G	C1160C (TGT→TGC)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,075,403	0	A	G	100.0%	194.8 / NA	52	C1160C (TGT→TGC)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/24);  total (28/24)

CGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGC  >  NC_000913/1075274‑1075532                                                                                                                                  |                                                                                                                                  cgcATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCt                                                                                                                         >  2:1050392/1‑140 (MQ=255)      ccTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAAt                                                                                                                    >  2:558402/1‑140 (MQ=255)       cTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAAtt                                                                                                                   >  1:74616/1‑140 (MQ=255)          cTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTg                                                                                                                >  1:857397/1‑140 (MQ=255)             atcatcGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGAc                                                                                                             <  2:754563/140‑1 (MQ=255)                atcGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGAt                                                                                                          >  1:550139/1‑140 (MQ=255)                  cGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGAttt                                                                                                        >  1:846056/1‑140 (MQ=255)                             aacaCGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTc                                                                                             >  2:992188/1‑140 (MQ=255)                             aacaCGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTc                                                                                             <  1:992188/140‑1 (MQ=255)                               cacGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTccc                                                                                           <  1:1050392/140‑1 (MQ=255)                               cacGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTccc                                                                                           >  2:429924/1‑140 (MQ=255)                               cacGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTccc                                                                                           <  1:801978/140‑1 (MQ=255)                               cacGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTccc                                                                                           >  1:534340/1‑140 (MQ=255)                                  gcgcTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCa                                                                                        >  2:136452/1‑140 (MQ=255)                                  gcgcTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCa                                                                                        <  1:846669/140‑1 (MQ=255)                                  gcgcTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCa                                                                                        >  1:362783/1‑140 (MQ=255)                                    gcTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGt                                                                                      >  2:36389/1‑140 (MQ=255)                                    gcTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGCTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGt                                                                                      >  1:368061/1‑140 (MQ=255)                                               agcagcGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCgg                                                                           <  2:534340/140‑1 (MQ=255)                                                 cagcGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCt                                                                         <  2:118146/140‑1 (MQ=255)                                                          ggTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATg                                                                <  2:857397/140‑1 (MQ=255)                                                                gCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCtt                                                          >  1:919454/1‑140 (MQ=255)                                                                 cGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCgg                                                                           >  1:807842/1‑122 (MQ=255)                                                                 cGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCgg                                                                           <  2:807842/122‑1 (MQ=255)                                                                     cACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAAc                                                     >  2:898694/1‑140 (MQ=255)                                                                     cACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAAc                                                     >  2:743521/1‑140 (MQ=255)                                                                      aCCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACt                                                    >  1:90262/1‑140 (MQ=255)                                                                          gTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTgcgc                                                <  2:919454/140‑1 (MQ=255)                                                                           tCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCa                                               >  2:551270/1‑140 (MQ=255)                                                                            cGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCAt                                              <  1:576264/140‑1 (MQ=255)                                                                            cGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCAt                                              <  1:898694/140‑1 (MQ=255)                                                                                  cGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCg                                        >  2:482214/1‑140 (MQ=255)                                                                                   ggCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCgg                                       <  2:90262/140‑1 (MQ=255)                                                                                     cagcaATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGAt                                     <  1:104558/140‑1 (MQ=255)                                                                                     cagcaATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGAt                                     <  2:362783/140‑1 (MQ=255)                                                                                     cagcaATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGAt                                     <  2:101852/140‑1 (MQ=255)                                                                                              ttGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCAt                                              <  1:29385/122‑1 (MQ=255)                                                                                              ttGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCAt                                              >  2:29385/1‑122 (MQ=255)                                                                                               tgtgTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACttt                                >  2:359031/1‑135 (MQ=255)                                                                                               tgtgTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACttt                                <  1:359031/135‑1 (MQ=255)                                                                                                   ttCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGa                       <  2:846056/140‑1 (MQ=255)                                                                                                      ctgcctgcGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGAcgc                    >  1:847518/1‑140 (MQ=255)                                                                                                          ctgcGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGa                <  2:368061/140‑1 (MQ=255)                                                                                                          ctgcGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGa                <  1:252023/140‑1 (MQ=255)                                                                                                          ctgcGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGa                <  2:74616/140‑1 (MQ=255)                                                                                                          ctgcGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGa                <  2:755175/140‑1 (MQ=255)                                                                                                             cgcCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCg             >  2:569303/1‑140 (MQ=255)                                                                                                             cgcCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCg             >  1:937201/1‑140 (MQ=255)                                                                                                               ccAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGGCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTc           >  2:627735/1‑140 (MQ=255)                                                                                                                cAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTg                        >  1:71431/1‑126 (MQ=255)                                                                                                                cAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTg                        <  2:71431/126‑1 (MQ=255)                                                                                                                        cATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAgc  >  1:212775/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                  CGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGC  >  NC_000913/1075274‑1075532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑