Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,118,667	A→G	V127A (GTC→GCC)	yceJ ←	putative cytochrome b561

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,118,667	0	A	G	100.0%	237.8 / NA	63	V127A (GTC→GCC)	yceJ	putative cytochrome b561
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/31);  total (32/31)

CGTCTTTATCGATGAAATGATGCTTCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCG  >  NC_000913/1118530‑1118799                                                                                                                                          |                                                                                                                                     cgtcTTTATCGATGAAATGATGCTTCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCg                                                                                                                                    >  1:810082/1‑140 (MQ=255)   tcTTTATCGATGAAATGATGCTTCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTc                                                                                                                                  >  1:682768/1‑140 (MQ=255)      ttATCGATGAAATGATGCTTCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCaaa                                                                                                                               <  1:847765/140‑1 (MQ=255)             tGAAATGATGCTTCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCa                                                                                                                        >  1:472779/1‑140 (MQ=255)              gAAATGATGCTTCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCaa                                                                                                                       >  1:456050/1‑140 (MQ=255)                      gCTTCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTg                                                                                                               >  1:804393/1‑140 (MQ=255)                        ttCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGAt                                                                                                             <  2:472779/140‑1 (MQ=255)                        ttCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGAt                                                                                                             <  1:365977/140‑1 (MQ=255)                         tCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATc                                                                                                            >  1:256634/1‑140 (MQ=255)                            gggCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGt                                                                                                         <  1:474799/140‑1 (MQ=255)                                  tAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTAcga                                                                                                   >  2:719973/1‑138 (MQ=255)                                   aaaTCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCAt                                                                                                  <  1:719973/140‑1 (MQ=255)                                      tCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCgg                                                                                               <  2:810082/140‑1 (MQ=255)                                      tCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCgg                                                                                               <  1:392584/140‑1 (MQ=255)                                       ccGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGc                                                                                              >  2:159639/1‑140 (MQ=255)                                          tGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGATTTACCATCGGCAGt                                                                                           <  2:86172/140‑1 (MQ=255)                                              tAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTgag                                                                                       <  1:91919/140‑1 (MQ=255)                                               aaCGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTgaga                                                                                      >  1:454487/1‑140 (MQ=255)                                               aaCGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTgaga                                                                                      <  2:1011502/140‑1 (MQ=255)                                                        tactacGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGc                                                                             >  2:547823/1‑140 (MQ=255)                                                        tactacGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGc                                                                             <  1:230716/140‑1 (MQ=255)                                                           tacGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGc                                                                          >  1:73909/1‑140 (MQ=255)                                                            acgacgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCt                                                                         <  2:671791/140‑1 (MQ=255)                                                             cgacgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTg                                                                        >  2:878996/1‑140 (MQ=255)                                                                cgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATg                                                                     >  1:131306/1‑140 (MQ=255)                                                                  cTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATa                                                                               <  1:489019/128‑1 (MQ=255)                                                                  cTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATa                                                                               >  2:489019/1‑128 (MQ=255)                                                                  cTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGcc                                                                   >  1:37823/1‑140 (MQ=255)                                                                  cTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGcc                                                                   >  1:1047683/1‑140 (MQ=255)                                                                  cTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGcc                                                                   >  2:769143/1‑140 (MQ=255)                                                                  cTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGcc                                                                   >  2:570405/1‑140 (MQ=255)                                                                  cTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGcc                                                                   >  2:1074/1‑140 (MQ=255)                                                                  cTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGcc                                                                   >  1:732004/1‑140 (MQ=255)                                                                  cTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGcc                                                                   >  1:600928/1‑140 (MQ=255)                                                                  cTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGcc                                                                   >  1:795573/1‑140 (MQ=255)                                                                      aCGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATg                                                               <  2:256634/140‑1 (MQ=255)                                                                               aaaaaTGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGc                                                      <  2:600928/140‑1 (MQ=255)                                                                                 aaaTGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCaa                                                    <  1:547823/140‑1 (MQ=255)                                                                                  aaTGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAAt                                                   <  1:769143/140‑1 (MQ=255)                                                                                      cAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGTCGATGGCGAAAAGCAATAGGt                                               <  2:845657/140‑1 (MQ=255)                                                                                      cAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGt                                               <  1:947873/140‑1 (MQ=255)                                                                                      cAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGt                                               <  1:534399/140‑1 (MQ=255)                                                                                      cAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGt                                               <  2:131306/140‑1 (MQ=255)                                                                                      cAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGt                                               >  2:620238/1‑140 (MQ=255)                                                                                           cACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAAt                                          >  2:852267/1‑140 (MQ=255)                                                                                              cGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCg                                       <  2:1047683/140‑1 (MQ=255)                                                                                                gCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCgag                                     <  2:601652/140‑1 (MQ=255)                                                                                                    aGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGt                                 >  1:1038767/1‑140 (MQ=255)                                                                                                        tGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAg                             >  2:307708/1‑140 (MQ=255)                                                                                                          ccTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGct                           >  2:870999/1‑140 (MQ=255)                                                                                                           cTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGctc                          <  2:37823/140‑1 (MQ=255)                                                                                                               gcgcCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGc                      >  2:152802/1‑140 (MQ=255)                                                                                                               gcgcCAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGc                      >  1:175615/1‑140 (MQ=255)                                                                                                                  ccAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGc                   <  1:307708/140‑1 (MQ=255)                                                                                                                  ccAGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGc                   <  1:152802/140‑1 (MQ=255)                                                                                                                    aGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCaa                 >  1:437647/1‑140 (MQ=255)                                                                                                                    aGCGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCaa                 <  1:570405/140‑1 (MQ=255)                                                                                                                      cGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGa               >  2:881746/1‑140 (MQ=255)                                                                                                                      cGTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGa               <  1:427323/140‑1 (MQ=255)                                                                                                                       gTCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGAc              <  2:682768/140‑1 (MQ=255)                                                                                                                        tCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGc                   >  1:1043074/1‑134 (MQ=255)                                                                                                                        tCGGCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGc                   <  2:1043074/134‑1 (MQ=255)                                                                                                                           gCAAGGGTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGt          <  2:454487/140‑1 (MQ=255)                                                                                                                                ggTCGCGGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATgg     >  1:405249/1‑140 (MQ=255)                                                                                                                                   cgcgGGGGCGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGGTCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCg  >  2:977183/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     CGTCTTTATCGATGAAATGATGCTTCAGGGCCATAAATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAACGCTGATCGGTTTACCATCGGCAGTTGAGATCAGATAGCCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCG  >  NC_000913/1118530‑1118799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑