Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,199,214	A→G	T174A (ACA→GCA)	lit →	e14 prophage; cell death peptidase Lit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,199,214	0	A	G	100.0%	250.0 / NA	66	T174A (ACA→GCA)	lit	e14 prophage; cell death peptidase Lit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/29);  total (37/29)

AATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGTGCTTTGTATACA  >  NC_000913/1199080‑1199347                                                                                                                                       |                                                                                                                                      aaTGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTgcagca                                                                                                                                  <  2:28919/140‑1 (MQ=255)      cccAAGCCAGAAGCATATTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATtctc                                                                                                                             <  2:823974/140‑1 (MQ=255)      cccAAGCCAGAAGCATATTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATtctc                                                                                                                             <  2:287278/140‑1 (MQ=255)               gAAGCATATTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAaga                                                                                                                    >  2:316240/1‑140 (MQ=255)                aaGCATATTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAagag                                                                                                                   <  1:316240/140‑1 (MQ=255)                     tatTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCg                                                                                                              <  2:147746/140‑1 (MQ=255)                     tatTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCg                                                                                                              >  1:194430/1‑140 (MQ=255)                     tatTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCg                                                                                                              >  1:147746/1‑140 (MQ=255)                     tatTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCg                                                                                                              >  2:259666/1‑140 (MQ=255)                      atTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAaga                                                                                                                    >  2:384203/1‑133 (MQ=255)                      atTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAaga                                                                                                                    <  1:384203/133‑1 (MQ=255)                      atTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGt                                                                                                             >  2:958812/1‑140 (MQ=255)                       tttACAAGGAAGCGAAGACTCACATGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTg                                                                                                            >  2:442587/1‑140 (MQ=255)                        ttACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGa                                                                                                           <  1:82010/140‑1 (MQ=255)                         tACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGaa                                                                                                          <  2:663661/140‑1 (MQ=255)                           cAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGc                                                                                                        >  1:304005/1‑140 (MQ=255)                              ggAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGa                                                                                                     >  2:299883/1‑140 (MQ=255)                              ggAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGa                                                                                                     >  2:968863/1‑140 (MQ=255)                                aaGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAg                                                                                                         >  1:342862/1‑134 (MQ=255)                                aaGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAg                                                                                                         <  2:342862/134‑1 (MQ=255)                                 aGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTc                                                                                                  >  2:214563/1‑140 (MQ=255)                                 aGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTc                                                                                                  >  2:153000/1‑140 (MQ=255)                                 aGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTc                                                                                                  >  2:208250/1‑140 (MQ=255)                                 aGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTc                                                                                                  >  1:298702/1‑140 (MQ=255)                                 aGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTc                                                                                                  >  1:308485/1‑140 (MQ=255)                                 aGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTc                                                                                                  >  1:364916/1‑140 (MQ=255)                                 aGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTc                                                                                                  >  1:733254/1‑140 (MQ=255)                                  gCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTca                                                                                                 <  2:1058436/140‑1 (MQ=255)                                    gAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTcaca                                                                                               <  2:733254/140‑1 (MQ=255)                                          tCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTAc                                                                                         >  2:85075/1‑140 (MQ=255)                                            acaAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACa                                                                                        <  1:878296/139‑1 (MQ=255)                                            acaAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACa                                                                                        >  2:878296/1‑139 (MQ=255)                                                    cTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGata                                                                               >  2:735303/1‑140 (MQ=255)                                                        cGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAg                                                                           <  2:837587/140‑1 (MQ=255)                                                          agaTATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGc                                                                         <  2:928653/140‑1 (MQ=255)                                                           gaTATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCa                                                                        <  1:70972/140‑1 (MQ=255)                                                             tatTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAAc                                                                      <  2:298702/140‑1 (MQ=255)                                                             tatTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAAc                                                                      <  1:214563/140‑1 (MQ=255)                                                                     tgtgCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGa                                                              >  1:493012/1‑140 (MQ=255)                                                                        gCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATc                                                           >  1:323914/1‑140 (MQ=255)                                                                             tGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTc                                                      <  2:493012/140‑1 (MQ=255)                                                                                 tGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGa                                                  >  1:677425/1‑140 (MQ=255)                                                                                        ttcATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAg                                           >  2:1025311/1‑140 (MQ=255)                                                                                         tcatcaTGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGa                                          >  2:550984/1‑140 (MQ=255)                                                                                          catcatGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGaa                                         >  2:727952/1‑140 (MQ=255)                                                                                             catGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACg                                      >  1:263014/1‑140 (MQ=255)                                                                                             catGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACg                                      >  2:425153/1‑140 (MQ=255)                                                                                               tGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTg                                    >  1:754156/1‑140 (MQ=255)                                                                                                       gTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGc                            <  1:299883/140‑1 (MQ=255)                                                                                                       gTCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGc                            >  1:688959/1‑140 (MQ=255)                                                                                                        tCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCa                           >  2:298006/1‑140 (MQ=255)                                                                                                        tCATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCa                           >  1:951624/1‑140 (MQ=255)                                                                                                         cATGTTGTTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCAt                          <  1:550984/140‑1 (MQ=255)                                                                                                           tgttgtTTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTg                        >  2:940452/1‑140 (MQ=255)                                                                                                              tgttTTACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTa                     <  1:735303/140‑1 (MQ=255)                                                                                                                  ttACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGc                 >  1:51870/1‑140 (MQ=255)                                                                                                                   tACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCa                <  1:229028/140‑1 (MQ=255)                                                                                                                   tACAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCa                <  2:951624/140‑1 (MQ=255)                                                                                                                     cAGCATCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGt              <  1:153000/140‑1 (MQ=255)                                                                                                                         aTCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGTGCtt          <  2:263014/140‑1 (MQ=255)                                                                                                                         aTCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGTGCtt          <  1:85075/140‑1 (MQ=255)                                                                                                                          tCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGTGCttt         >  2:669628/1‑140 (MQ=255)                                                                                                                          tCCATTGGTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGTGCttt         >  2:615735/1‑140 (MQ=255)                                                                                                                                 gTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGTGCTTTGTATACa  <  2:304005/140‑1 (MQ=255)                                                                                                                                 gTCACTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGTGCTTTGTATACa  <  1:958812/140‑1 (MQ=255)                                                                                                                                    aCTGCAGCATTCTCCACTCAAGGGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGTGCTTTGtata    <  2:621041/135‑1 (MQ=255)                                                                                                                                    aCTGCAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGTGCTTTGtata    <  2:574218/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      AATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACATGCTACAAAATGGATATTAGGCAACCTGTATGAATCCGCTCCTGAATTAAAGAAACGTGCACTTGGCATTGCTACGGCAGTGCTTTGTATACA  >  NC_000913/1199080‑1199347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑