Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,274,477	A→G	T232A (ACA→GCA)	ychO →	putative invasin YchO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,274,477	0	A	G	100.0%	259.2 / NA	69	T232A (ACA→GCA)	ychO	putative invasin YchO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/39);  total (30/39)

GGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTA  >  NC_000913/1274353‑1274611                                                                                                                             |                                                                                                                                       gggCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCa                                                                                                                         >  2:690496/1‑140 (MQ=255)  ggCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCAt                                                                                                                        <  2:134446/140‑1 (MQ=255)   gCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATg                                                                                                                       <  2:473284/140‑1 (MQ=255)     tttGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGcc                                                                                                                     <  1:185556/140‑1 (MQ=255)      ttGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGTCGCGCGGGTACGCCCTGGCAGCTCGGATGCGCATGCCg                                                                                                                    <  1:777480/140‑1 (MQ=255)          tGCCGAAGCGT‑GGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTa                                                                                                               >  1:663846/1‑140 (MQ=255)             cGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATc                                                                                                             >  1:174153/1‑140 (MQ=255)             cGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATc                                                                                                             <  1:399059/140‑1 (MQ=255)               aaGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCaa                                                                                                           <  2:331899/140‑1 (MQ=255)               aaGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGCTCTATCaa                                                                                                           <  1:933509/140‑1 (MQ=255)               aaGCGT‑GGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAac                                                                                                          <  2:663846/140‑1 (MQ=255)                aGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCCGGCAGCTCGGATGCGCATGCCGTTCTATCAac                                                                                                          <  2:766480/140‑1 (MQ=255)                    tGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGcc                                                                                                                     <  2:146230/125‑1 (MQ=255)                    tGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGcc                                                                                                                     >  1:146230/1‑125 (MQ=255)                    tGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCt                                                                                                      >  2:1030878/1‑140 (MQ=255)                    tGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCt                                                                                                      >  1:243947/1‑140 (MQ=255)                            atatTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCt                                                                                                                <  2:12303/122‑1 (MQ=255)                            atatTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCt                                                                                                                >  1:12303/1‑122 (MQ=255)                                ttGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAgtgt                                                                                          <  2:410460/140‑1 (MQ=255)                                 tGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTc                                                                                         >  1:47108/1‑140 (MQ=255)                                  gCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCa                                                                                        >  2:585596/1‑140 (MQ=255)                                     aTTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGcc                                                                                     >  1:114148/1‑140 (MQ=255)                                      ttATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCt                                                                                    >  2:608775/1‑140 (MQ=255)                                       tATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAgtgt                                                                                          >  2:315834/1‑133 (MQ=255)                                       tATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAgtgt                                                                                          <  1:315834/133‑1 (MQ=255)                                       tATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTa                                                                                   >  1:903446/1‑140 (MQ=255)                                        aTCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCa                                                                                                    >  1:357080/1‑122 (MQ=255)                                        aTCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCa                                                                                                    <  2:357080/122‑1 (MQ=255)                                         tCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGa                                                                                 >  1:589731/1‑140 (MQ=255)                                          cGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGaa                                                                                <  1:414684/140‑1 (MQ=255)                                                aCTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAgtgt                                                                                          <  1:568237/124‑1 (MQ=255)                                                aCTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAgtgt                                                                                          >  2:568237/1‑124 (MQ=255)                                                aCTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTAt                                                                          <  2:669617/140‑1 (MQ=255)                                                  ttttATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTAttt                                                                        <  1:846284/140‑1 (MQ=255)                                                   tttATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTAtttt                                                                       <  1:676000/140‑1 (MQ=255)                                                       tCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTg                                                                   <  2:905472/140‑1 (MQ=255)                                                            cGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCgt                                                              >  2:847576/1‑140 (MQ=255)                                                              tttGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCGGCCTAGAACAGTATTTTGGTGATCgtgt                                                            >  1:789049/1‑140 (MQ=255)                                                                     catggcatgAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTg                                                     >  1:527687/1‑140 (MQ=255)                                                                        ggcatgAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCGGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGttt                                                  <  2:789049/140‑1 (MQ=255)                                                                        ggcatgAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGttt                                                  <  1:1030878/140‑1 (MQ=255)                                                                           atgAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAAc                                               <  1:422011/140‑1 (MQ=255)                                                                           atgAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAAc                                               <  2:1015489/140‑1 (MQ=255)                                                                            tgAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAAct                                              <  1:608775/140‑1 (MQ=255)                                                                               acagacagCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTg                                           >  1:883579/1‑140 (MQ=255)                                                                                  gacagCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTa                                        <  1:690496/140‑1 (MQ=255)                                                                                  gacagCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTACTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTa                                        <  1:245971/140‑1 (MQ=255)                                                                                  gacacCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTa                                        <  2:974980/140‑1 (MQ=255)                                                                                   acagCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTAc                                       >  1:890495/1‑140 (MQ=255)                                                                                    cagCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACg                                      <  1:847576/140‑1 (MQ=255)                                                                                       ccACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGt                                   >  2:496208/1‑140 (MQ=255)                                                                                       ccACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGt                                   <  1:585596/140‑1 (MQ=255)                                                                                        cACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTa                                        <  2:1016658/134‑1 (MQ=255)                                                                                        cACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTa                                        >  1:1016658/1‑134 (MQ=255)                                                                                        cACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGtt                                  >  1:407306/1‑140 (MQ=255)                                                                                          cGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAAct                                              <  1:208464/126‑1 (MQ=255)                                                                                          cGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAAct                                              >  2:208464/1‑126 (MQ=255)                                                                                          cGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAAc                                               >  2:589029/1‑125 (MQ=255)                                                                                          cGCAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAAc                                               <  1:589029/125‑1 (MQ=255)                                                                                            cAGGAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATca                              >  1:286084/1‑140 (MQ=255)                                                                                               gAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTATGGGTTATCACaa                           <  2:243947/140‑1 (MQ=255)                                                                                               gAACAACGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACaa                           <  2:952975/140‑1 (MQ=255)                                                                                                 acaacGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATc                         >  2:296411/1‑140 (MQ=255)                                                                                                     cGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGt                     <  1:496208/140‑1 (MQ=255)                                                                                                     cGGATGGCGCGCGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGt                     >  2:831543/1‑140 (MQ=255)                                                                                                           gcgcgcGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGtt               >  1:60054/1‑140 (MQ=255)                                                                                                           gcgcgcGGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGtt               <  2:573927/140‑1 (MQ=255)                                                                                                                cgGGTACGACCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTc          <  1:296411/140‑1 (MQ=255)                                                                                                                        aCCTGGCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTa  <  2:174153/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                       GGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTA  >  NC_000913/1274353‑1274611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑