Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,318,581	A→G	V403A (GTT→GCT)	trpC ←	fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,318,581	0	A	G	100.0%	168.5 / NA	45	V403A (GTT→GCT)	trpC	fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/20);  total (25/20)

AAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCGACGCTTAATGCTTT  >  NC_000913/1318450‑1318717                                                                                                                                    |                                                                                                                                         aaaCCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCa                                                                                                                                  >  1:299174/1‑140 (MQ=255)   aCCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAg                                                                                                                                >  2:803663/1‑140 (MQ=255)     cGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCg                                                                                                                              >  2:1043430/1‑140 (MQ=255)          ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGa                                                                                                                         <  2:924464/140‑1 (MQ=255)          ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGa                                                                                                                         <  2:286622/140‑1 (MQ=255)          ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGa                                                                                                                         <  2:131095/140‑1 (MQ=255)          ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGa                                                                                                                         <  1:46866/140‑1 (MQ=255)          ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGa                                                                                                                         <  1:803663/140‑1 (MQ=255)          ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGa                                                                                                                         <  1:707809/140‑1 (MQ=255)               aGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCAtt                                                                                                                    <  1:1043430/140‑1 (MQ=255)                   gTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCa                                                                                                                                  >  2:835560/1‑122 (MQ=255)                   gTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCa                                                                                                                                  <  1:835560/122‑1 (MQ=255)                   gTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAAt                                                                                                                >  2:255671/1‑140 (MQ=255)                   gTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAAt                                                                                                                >  1:789632/1‑140 (MQ=255)                      cGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGCTGCCAAGCGATTGACCATTTAATAGt                                                                                                             >  1:101939/1‑140 (MQ=255)                                 cccGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCaaa                                                                                                  >  1:948300/1‑140 (MQ=255)                                   cGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAAc                                                                                                >  2:770107/1‑140 (MQ=255)                                     gTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGt                                                                                              >  1:931101/1‑140 (MQ=255)                                      ttGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGtt                                                                                             >  2:6579/1‑140 (MQ=255)                                         cGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGCTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGcc                                                                                          <  2:101939/140‑1 (MQ=255)                                           aCTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCg                                                                                        >  1:901667/1‑140 (MQ=255)                                              ttACAGCAAAATTAAAATCAAGTCCGGCGCAGCCGTTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCtt                                                                                     <  2:931101/139‑1 (MQ=255)                                                       aaTTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTgg                                                                            >  1:567501/1‑140 (MQ=255)                                                            aaaTCAAGCCCGGCGCACCCGGTTTGTGCGCCTTCCACGCAGTTACCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAGCGTTGCCCGCTTCCACCCTGGCCGtt                                                                       <  2:819525/140‑1 (MQ=255)                                                                   gTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTaaa                                                                >  2:919132/1‑140 (MQ=255)                                                                     ccGGCGCAGCCGGTTTGTGCCGCTTCCCCGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAAc                                                              <  2:398086/140‑1 (MQ=255)                                                                          gcAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATAtt                                                         <  2:901667/140‑1 (MQ=255)                                                                               cGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCa                                                    <  1:965798/140‑1 (MQ=255)                                                                               cGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCa                                                    <  1:770107/140‑1 (MQ=255)                                                                                      tgCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCt                                             >  2:954826/1‑140 (MQ=255)                                                                                                      ttATCTGCGCCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGcgggcggg                             <  1:481323/140‑1 (MQ=255)                                                                                                            gcgcCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGt                       >  2:370773/1‑140 (MQ=255)                                                                                                              gcCTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGttt                     >  1:460887/1‑140 (MQ=255)                                                                                                                cTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCa                   >  1:417366/1‑140 (MQ=255)                                                                                                                cTAAGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCa                   >  1:980922/1‑140 (MQ=255)                                                                                                                   gcccccccGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCg                <  2:786535/138‑1 (MQ=255)                                                                                                                   aGCCCCCCGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCg                <  2:460887/140‑1 (MQ=255)                                                                                                                     ccccccGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAg                          >  2:195824/1‑128 (MQ=255)                                                                                                                     ccccccGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAg                          <  1:195824/128‑1 (MQ=255)                                                                                                                       ccccGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCGACGc            >  2:664731/1‑140 (MQ=255)                                                                                                                          cGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCTACGCTTa         <  2:567501/140‑1 (MQ=255)                                                                                                                          cGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCGACGCTTa         >  1:385657/1‑140 (MQ=255)                                                                                                                          cGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCGACGCTTa         >  2:519298/1‑140 (MQ=255)                                                                                                                          cGCCAGCAGAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCGACGCTTa         >  2:1028011/1‑140 (MQ=255)                                                                                                                                cagAGCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCGACGCTTAATGCtt   >  2:2234/1‑140 (MQ=255)                                                                                                                                 agagCGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCGACGCTTAATGCttt  <  1:519298/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         AAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCGACGCTTAATGCTTT  >  NC_000913/1318450‑1318717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑