Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,348,715	A→G	S66S (AGT→AGC)	rnb ←	RNase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,348,715	0	A	G	100.0%	147.0 / NA	40	S66S (AGT→AGC)	rnb	RNase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/14);  total (26/14)

CAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCACTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAG  >  NC_000913/1348578‑1348848                                                                                                                                          |                                                                                                                                      cAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCt                                                                                                                                     >  1:264884/1‑140 (MQ=255)                     gagTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTc                                                                                                                 >  2:846524/1‑140 (MQ=255)                      agTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCg                                                                                                                <  2:264884/140‑1 (MQ=255)                      agTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCg                                                                                                                <  1:846524/140‑1 (MQ=255)                         ggATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTc                                                                                                             >  2:553848/1‑140 (MQ=255)                          gatgatCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCg                                                                                                            >  2:481331/1‑140 (MQ=255)                          gatgatCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCg                                                                                                            >  1:160607/1‑140 (MQ=255)                          gatgatCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCg                                                                                                            <  1:481331/140‑1 (MQ=255)                                 aGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCcatgca                                                                                                     <  1:1013583/140‑1 (MQ=255)                                        aTGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACttt                                                                                              >  1:897552/1‑140 (MQ=255)                                        aTGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACtt                                                                                               >  2:828114/1‑139 (MQ=255)                                        aTGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACtt                                                                                               <  1:828114/139‑1 (MQ=255)                                          ggCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACttttt                                                                                            >  1:568440/1‑140 (MQ=255)                                                       tttttGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTgcggcgg                                                                               <  1:553848/140‑1 (MQ=255)                                                          ttGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGcggcggcgg                                                                            >  1:154668/1‑140 (MQ=255)                                                                  aaCCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAAt                                                                    >  2:970558/1‑140 (MQ=255)                                                                              gAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTgcg                                                        >  2:305005/1‑140 (MQ=255)                                                                              gAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTgcg                                                        >  2:252334/1‑140 (MQ=255)                                                                                 aCGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGt                                                     <  2:160607/140‑1 (MQ=255)                                                                                  cGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTc                                                    <  2:314956/140‑1 (MQ=255)                                                                                               gTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAg                                       >  2:939029/1‑140 (MQ=255)                                                                                               gTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAg                                       >  1:843113/1‑140 (MQ=255)                                                                                                ttCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGTAATAACTTTTTTGCGCGTCGACTTCCAGGAAGc                                      >  1:140355/1‑140 (MQ=255)                                                                                                  cAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCa                                    <  1:305005/140‑1 (MQ=255)                                                                                                    aCCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCaaa                                  >  1:11497/1‑140 (MQ=255)                                                                                                         ttcttcTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCtt                             >  2:271669/1‑140 (MQ=255)                                                                                                         ttcttcTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTATTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCtt                             >  2:656187/1‑140 (MQ=255)                                                                                                                 gCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCtgtg                     >  1:316398/1‑140 (MQ=255)                                                                                                                   tctGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGc                   >  1:899425/1‑140 (MQ=255)                                                                                                                   tctGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGc                   >  1:775763/1‑140 (MQ=255)                                                                                                                    ctGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCt                  <  2:430102/140‑1 (MQ=255)                                                                                                                    ctGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCt                  >  2:900180/1‑140 (MQ=255)                                                                                                                     tGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAAAGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCtt                 >  2:849739/1‑140 (MQ=255)                                                                                                                       cGGATTCACGTTCTTTTTTGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCtttt               >  1:252901/1‑140 (MQ=255)                                                                                                                       cGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCtttt               >  1:405941/1‑140 (MQ=255)                                                                                                                       cGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCt                  >  2:21601/1‑137 (MQ=255)                                                                                                                       cGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCt                  <  1:21601/137‑1 (MQ=255)                                                                                                                             cACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTaccacc         <  2:405941/140‑1 (MQ=255)                                                                                                                                gTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCt      <  1:851461/140‑1 (MQ=255)                                                                                                                                    ttttttGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAg  <  2:252901/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      CAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCACTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAG  >  NC_000913/1348578‑1348848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑